A5043455577- Antibiotic Resistance in Bacteria
- Reproductive Biology and Fertility
- Enterobacteriaceae and Cronobacter Research
- Salmonella and Campylobacter epidemiology
- Assisted Reproductive Technology and Twin Pregnancy
- Reproductive Health and Technologies
- Mitochondrial Function and Pathology
- Sperm and Testicular Function
- Prenatal Screening and Diagnostics
- Infections and bacterial resistance
- Hereditary Neurological Disorders
- Metabolism and Genetic Disorders
- Migraine and Headache Studies
- Antibiotics Pharmacokinetics and Efficacy
- Plant Pathogenic Bacteria Studies
- Pneumonia and Respiratory Infections
- Ovarian function and disorders
- Bacterial biofilms and quorum sensing
- Pharmaceutical and Antibiotic Environmental Impacts
- Antibiotic Use and Resistance
- Escherichia coli research studies
- Tuberculosis Research and Epidemiology
- Neurological diseases and metabolism
- Vector-Borne Animal Diseases
- Congenital heart defects research
Istituto Superiore di Sanità
2012-2023
University of Verona
2023
Istituti di Ricovero e Cura a Carattere Scientifico
2001-2022
Agostino Gemelli University Polyclinic
2019-2022
University of Padua
2008
Fondazione FADOI
2001-2005
Sapienza University of Rome
1997-2003
Centro Medicina della Riproduzione
2003
University of Rome Tor Vergata
2002
Reproductive Medicine Institute
1996-2000
IncF plasmids are frequently encountered in clinical enterobacterial strains associated with the dissemination of relevant antimicrobial resistance and virulence genes. These usually heterogeneous size carry multiple replicons, technical difficulties can impair comparison detection related by restriction fragment length polymorphism analysis. We devised a rapid sequence-based typing scheme to categorize members this plasmid family into homogeneous groups. compared available replicon...
The whole-genome sequence of an epidemic, multidrug-resistant Acinetobacter baumannii strain (strain ACICU) belonging to the European clone II group and carrying plasmid-mediated bla(OXA)(-)(58) carbapenem resistance gene was determined. A. ACICU genome compared with genomes ATCC 17978 baylyi ADP1, aim identifying novel genes related virulence drug resistance. has a single chromosome 3,904,116 bp (which is predicted contain 3,758 genes) two plasmids, pACICU1 pACICU2, 28,279 64,366 bp,...
The aim of this study was to identify and characterize plasmids carrying qnrS1, qnrB2 qnrB19 genes identified in Salmonella strains from Netherlands. identification may help follow the dissemination these resistance different countries environments. Plasmids 33 qnr-positive were transferred Escherichia coli analysed by restriction, Southern blot hybridization, PCR sequencing determinants. They also assigned incompatibility groups PCR-based replicon typing, including three additional assays...
Plasmids belonging to incompatibility group I1 (IncI1) are widespread in Enterobacteriaceae and characterized by the presence of a cluster genes encoding type IV pili, contributing virulence Shiga-toxigenic Escherichia coli. Recently, IncI1 plasmids were identified E. coli Salmonella strains animal origin as responsible for dissemination β-lactamase genes. Plasmid multilocus sequence typing (pMLST) was developed discern naturally occurring homogeneous groups according their allele...
The global spread of Klebsiella pneumoniae producing carbapenemase (KPC) has been mainly associated with the dissemination high-risk clones. In last decade, hospital outbreaks involving KPC-producing K. have predominantly attributed to isolates belonging clonal group (CG) 258. However, results recent epidemiological analysis indicate that sequence type (ST) 307, is emerging in different parts world and a candidate become prevalent clone near future. Here we show ST307 genome encodes genetic...
ABSTRACT Full genome sequences were determined for five Klebsiella pneumoniae strains belonging to the sequence type 512 (ST512) clone, producing KPC-3. Three resistant tigecycline, one showed an intermediate phenotype, and was susceptible. Comparative analysis performed using of susceptible strain as a reference identified genetic differences possibly associated with resistance tigecycline. Results demonstrated that mutations in ramR gene occurred two three sequenced strains. Mutations RamR...
In a prospective randomized study, we analysed 125 patients at risk of ovarian hyperstimulation syndrome (OHSS), selected in the period between January 1996 and July 1997. All had blood oestradiol concentration ⩾1500 pg/ml on day human chorionic gonadotrophin (HCG) administration ⩾15 oocytes were collected. The matched two groups: group A, control (n = 67), fresh embryo transfers; B 58) cryopreservation all obtained pronucleate embryos. Pregnancy, live birth rates incidence OHSS compared...
Escherichia coli strains producing extended-spectrum beta-lactamases (ESBLs) are a major problem in many different hospitals worldwide, causing outbreaks as well sporadic infections. The prevalence of ESBL producers was analyzed surveillance study performed on the population attending Policlinico Umberto I, largest university hospital Rome, Italy. We also investigated genotypes, pathogenicity islands, and plasmids ESBL-positive E. isolates further markers that useful describing epidemiology...
ObjectivesThe animal reservoir of plasmid-mediated quinolone resistance (PMQR) and β-lactamases is still controversial little information available on the prevalence these determinants in developing countries. The aim this study was to identify characterize PMQR a collection commensal ampicillin-resistant Escherichia coli isolated from healthy chickens pigs at slaughter, collected November–December 2006, Ibadan, Nigeria.
The purpose of the present study was to evaluate effect cryopreservation on 55 embryos which had one blastomere biopsied for preimplantation genetic diagnosis aneuploidy before freezing. thawing outcome compared that obtained in 94 derived from our conventional freezing programme patients with comparable characteristics who were treated same period. Their morphologically similar but incidence 100% biopsy group and unknown controls. percentage survived intact after significantly lower...
The effect of the assisted zona hatching (AZH) procedure was investigated on 135 cycles with a poor prognosis pregnancy due to: (i) maternal age > or = 38 years (45 cycles); (ii) three more failed in-vitro fertilization (IVF) attempts (70 cycles), and (iii) patients possessing both inclusion criteria (20 cycles). control groups (113 cycles) included same characteristics (42, 53 18 respectively) who did not undergo AZH procedure. A total 505 embryos treated before transfer, resulting in: 14,...
The mechanisms at the origin of heterogeneous carbapenem resistance levels observed among Acinetobacter baumannii isolates collected in 2005 a large University Hospital Rome, Italy, were investigated. These related and possessed similar plasmids carrying carbapenem-hydrolyzing oxacillinase gene bla(OXA-58) but showed variable to carbapenems. Analysis sequences surrounding genetic variability, with presence several multiple copies gene; this extra copy number was likely an IS26-mediated...
Basilar migraine (BM), familial hemiplegic (FHM), and sporadic (SHM) are phenotypically similar subtypes of with aura, differentiated only by motor symptoms, which absent in BM. Mutations CACNA1A ATP1A2 have been found FHM. The authors detected a novel mutation the gene (R548H) members family BM, suggesting that BM FHM may be allelic disorders.
ObjectivesWe report the case of a kidney-transplant patient, suffering an intra-abdominal abscess at surgical site caused by carbapenem-resistant ST258 Klebsiella pneumoniae clone, producing KPC-3 carbapenemase. Under tigecycline treatment, patient developed sepsis carbapenem-susceptible K. strain. Complete DNA sequences plasmids carried resistant and susceptible strains from this were determined.
The entire plasmid content of a multidrug-resistant, CTX-M-15-producing Klebsiella pneumoniae ST416 clone was investigated. Two FII(K) plasmids, pKDO1 (127 kb) and pKPN-CZ (207 kb), were identified found to carry formidable set genes conferring resistance toxic compounds, metals, antimicrobial drugs exhibiting novel features putatively associated with adaptation fitness the bacterium in human host.
We describe the first isolation of an NDM-1-producing Pseudomonas aeruginosa in Italy. In May 2013, a patient with acute lymphoblastic leukaemia and history prior hospitalisation Belgrad, Serbia, underwent stem cell transplantation at tertiary care hospital Rome, After transplantion, sepsis by P. occurred, leading to septic shock fatal outcome.
Pandemic coronavirus disease 2019 (COVID-19) represents a challenge for healthcare structures. The molecular confirmation of samples from infected individuals is crucial and therefore guides public health decision making. Clusters possibly increased diffuse transmission could occur in the context next influenza season. For this reason, diagnostic test able to discriminate severe acute respiratory syndrome 2 (SARS-CoV-2) viruses urgently needed.
A collection of human-epidemiologically unrelated S. enterica strains collected over a 3-year period (2016 to 2018) in Italy by the national surveillance Enter-Net Italia was analysed.Antimicrobial susceptibility tests, including determination minimal inhibitory concentrations (MICs) for colistin, were performed. Colistin resistant analysed PCR detect mobile colistin resistance (mcr) genes. In mcr-negative serovar Enteritidis strains, chromosomal mutations potentially involved identified...