A5102933331- Legionella and Acanthamoeba research
- Viral gastroenteritis research and epidemiology
- Electron and X-Ray Spectroscopy Techniques
- Blood groups and transfusion
- Viral Infections and Immunology Research
- Computational Drug Discovery Methods
- Semiconductor materials and interfaces
- Surface and Thin Film Phenomena
- Hemoglobinopathies and Related Disorders
- Bacterial biofilms and quorum sensing
- Blood donation and transfusion practices
- Respiratory viral infections research
- Vibrio bacteria research studies
- Animal Virus Infections Studies
- Semiconductor Quantum Structures and Devices
- Drug-Induced Adverse Reactions
- Virus-based gene therapy research
- Semiconductor materials and devices
- Erythrocyte Function and Pathophysiology
- Immune Cell Function and Interaction
- SARS-CoV-2 and COVID-19 Research
- Hepatitis C virus research
- Mass Spectrometry Techniques and Applications
- Advanced X-ray Imaging Techniques
- Blood disorders and treatments
Istituto Superiore di Sanità
2015-2024
Aptuit (Italy)
2011-2024
Swiss Red Cross
2015-2024
University of Lausanne
2019-2023
Elettra-Sincrotrone Trieste S.C.p.A.
1995-2022
Consorzio del Formaggio Parmigiano-Reggiano
2019
Università della Svizzera italiana
2018
Sapienza University of Rome
2016
University Health Network
2014
University of British Columbia
2014
Allopurinol (ALP) hypersensitivity is a major cause of severe cutaneous adverse reactions and strongly associated with the HLA-B*58:01 allele. However, it can occur in absence this allele identical clinical manifestations. The immune mechanism ALP-induced poorly understood, T cell-reactivity pattern patients or without not known. To understand interactions among drug, HLA, TCR, we generated cell lines that react to ALP its metabolite oxypurinol (OXP) from HLA-B*58:01(+) HLA-B*58:01(-) donors...
Background Allopurinol is a main cause of severe cutaneous adverse reactions (SCAR). How allopurinol induces hypersensitivity remains unknown. Pre-disposing factors are the presence HLA-B*58:01 allele, renal failure and possibly dose taken. Objective Using an in vitro model, we sought to decipher relationship among metabolism, phenotype drug concentrations stimulating drug-specific T cells. Methods Lymphocyte transformation test (LTT) results patients who had developed were analysed. We...
Drug-induced liver injury (DILI) is a main cause of drug withdrawal. A particularly interesting example flucloxacillin (FLUX)-DILI, which associated with the HLA-B*57:01 allele. At present, mechanism FLUX-DILI not understood, but HLA association suggests role for activated T cells in pathomechanism damage. To understand interaction among FLUX, molecules, and cells, we generated FLUX-reacting from FLUX-naive HLA-B*57:01(+) HLA-B*57:01(-) healthy donors investigated cell stimulation. We found...
To perform an international trial to derive alert and action levels for the use of quantitative PCR (qPCR) in monitoring Legionella determine effectiveness control measures against legionellae. Laboratories (7) participated from six countries. Legionellae were determined by culture qPCR methods with comparable detection limits. Systems monitored over ≥10 weeks. For cooling towers (232 samples), there was a significant difference between log mean (GU l(-1) ) (CFU pneumophila (0·71) spp....
The antiretroviral drug abacavir (abc) elicits severe hypersensitivity reactions in HLA ‐ B *5701 + individuals. To understand the abc‐specific activation of CD 8 T cells, we generated ‐cell clones (abc‐ TCC s). Abc reactivity could not be linked to metabolism and/or processing drug, since abc metabolizing enzymes were expressed immune cells and inhibition proteasome APC s did affect reactivity. Ca 2+ influx assays revealed different patterns abc‐ s. While all reacted presented on molecules,...
Abacavir hypersensitivity is a severe reaction which occurs exclusively in carriers of the HLA-B*57∶01 allele. In vitro culture PBMC with abacavir results outgrowth abacavir-reacting CD8+ T cells, release IFNγ and are cytotoxic. How this immune response induced what recognized by these cells still matter debate. We analyzed conditions required to develop an abacavir-dependent cell vitro. The reactivity was independent co-stimulatory signals, as neither DC maturation nor inflammatory...
Aim: Decreased DPD activity is a major cause of 5-fluorouracil (5-FU) toxicity, but known reduced-function variants in the gene (DPYD) explain only part DPD-related 5-FU toxicities. Here, we evaluated baseline (pretherapeutic) plasma 5,6-dihydrouracil:uracil (UH2:U) ratio as marker context DPYD genotypes. Materials & methods: were genotyped and U, UH2 concentrations determined by liquid chromatography-tandem mass spectrometry 320 healthy blood donors 28 cancer patients receiving 5-FU-based...
Intestinal barrier derangement allows intestinal bacteria and their products to translocate the systemic circulation. Pseudomonas aeruginosa (PA) superimposed infection in critically ill patients increases gut permeability leads gut-driven sepsis. PA infections are challenging due multi-drug resistance (MDR), biofilms, and/or antibiotic tolerance. Inhibition of quorum-sensing transcriptional regulator MvfR(PqsR) is a desirable anti-PA anti-virulence strategy as MvfR controls multiple acute...
Noroviruses have received increased attention in recent years because their role as etiologic agents acute gastroenteritis outbreaks is now clearly established. Our inability to grow them cell culture and the lack of an animal model hinder characterization these viruses. More recently, molecular approaches been used study genetic relationships that exist among them. In present study, environmental samples from seawater, estuarine water, effluents sewage treatment plants were analyzed order...
The aim of this study was to identify risk variants and haplotypes that impair dihydropyrimidine dehydrogenase (DPD) activity are, therefore, candidate for severe toxicity 5-fluorouracil (5-FU) chemotherapy.Plasma dihydrouracil/uracil (UH2 /U) ratios were measured as a population marker DPD in total 1382 subjects from 4 independent studies. Genotype haplotype correlations with UH2 /U assessed.Significantly lower (panova < 2 × 10-16 ) observed carriers the well-studied 5-FU mean differences...
The discovery of new highly potent and selective dopamine (DA) D(3) receptor antagonists has recently allowed the characterization DA in a range preclinical animal models drug addiction. A novel series 1,2,4-triazol-3-yl-azabicyclo[3.1.0]hexanes, members which showed high affinity selectivity for excellent pharmacokinetic profiles, is reported here. Members group derivatives from this good oral bioavailability brain penetration very vitro receptor, as well potency antagonism at receptor....
The discovery of new highly potent and selective triple reuptake inhibitors is reported. classes 1-(aryl)-6-[alkoxyalkyl]-3-azabicyclo[3.1.0]hexanes 6-(aryl)-6-[alkoxyalkyl]-3-azabicyclo[3.1.0]hexanes are described together with detailed SAR. Appropriate decoration the scaffolds was achieved help a inhibitor pharmacophore model here. Selected derivatives showed good oral bioavailability (>30%) brain penetration (B/B > 4) in rats associated high vitro potency selectivity at SERT, NET, DAT....
Pandemic coronavirus disease 2019 (COVID-19) represents a challenge for healthcare structures. The molecular confirmation of samples from infected individuals is crucial and therefore guides public health decision making. Clusters possibly increased diffuse transmission could occur in the context next influenza season. For this reason, diagnostic test able to discriminate severe acute respiratory syndrome 2 (SARS-CoV-2) viruses urgently needed.
In the course of 1998, Spectromicroscopy beamline at ELETTRA completed commissioning and succeeded in performing its first test experiments. The is designed to perform photoemission experiments with high spatial resolution, which obtained by focusing radiation a small spot on sample means multilayer-coated Schwarzschild Objective. Three objectives are currently available; these operate photon energies 74, 95, 110 eV. A review presented performances achieved together an outlook future...
We report a low energy electron microscopy study of the relation between self-organized Ge/Si(111)nanostructures and their local environment. By comparison with Monte Carlo simulations, three-dimensional islands are shown to display substantial tendency towards self-ordering. This may result from diffusive nature nucleation processes. The size individual nanostructures does not significantly correlate distance neighboring islands. Thus energetic factors thought govern competition among...
Summary Porcine enteroviruses (PEVs) and teschoviruses (PTVs) are described as causative agents of neurological disorders, fertility disorders dermal lesions swine. Difficulties in the serological detection these viruses may lead to a significant underestimation infections with clinical symptoms. With recent availability genome sequence data for all serotypes, molecular diagnosis is possibility. The present study describes new approach ‘serotyping’ PTVs PEV‐B viruses, involving amplification...
Bioisoteric replacement of the metabolically labile N-methyl amide group a series benzoxazinones with small heterocyclic rings has led to novel fused tricyclic benzoxazines which are potent 5-HT1A/B/D receptor antagonists and without concomitant human serotonin transporter (hSerT) activity. Optimizing against multiple parameters in parallel identified 6-{2-[4-(2-methyl-5-quinolinyl)-1-piperazinyl]ethyl}-4H-imidazo[5,1-c][1,4]benzoxazine-3-carboxamide (GSK588045) as antagonist high degree...