A5077676515- RNA Research and Splicing
- RNA modifications and cancer
- Circular RNAs in diseases
- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- RNA and protein synthesis mechanisms
- Planarian Biology and Electrostimulation
- Muscle Physiology and Disorders
- SARS-CoV-2 and COVID-19 Research
- interferon and immune responses
- bioluminescence and chemiluminescence research
- Cell Image Analysis Techniques
- Genomics and Chromatin Dynamics
- Cephalopods and Marine Biology
- RNA regulation and disease
- Single-cell and spatial transcriptomics
- Mitochondrial Function and Pathology
- RNA Interference and Gene Delivery
- Genetic Neurodegenerative Diseases
- Cancer Cells and Metastasis
- Heat shock proteins research
- Marine animal studies overview
- Medical Imaging Techniques and Applications
- Photoreceptor and optogenetics research
- Developmental Biology and Gene Regulation
Max Delbrück Center
2019-2025
Human Technopole
2023-2025
MSB Medical School Berlin
2023
Helmholtz Association of German Research Centres
2022
Sapienza University of Rome
2011-2020
Istituto Pasteur
2010
Circular RNAs (circRNAs) constitute a family of transcripts with unique structures and still largely unknown functions. Their biogenesis, which proceeds via back-splicing reaction, is fairly well characterized, whereas their role in the modulation physiologically relevant processes unclear. Here we performed expression profiling circRNAs during vitro differentiation murine human myoblasts, identified conserved species regulated myogenesis altered Duchenne muscular dystrophy. A high-content...
Abstract The RNA-binding protein FUS participates in several RNA biosynthetic processes and has been linked to the pathogenesis of amyotrophic lateral sclerosis (ALS) frontotemporal dementia. Here we report that controls back-splicing reactions leading circular (circRNA) production. We identified circRNAs expressed vitro -derived mouse motor neurons (MNs) determined production a considerable number these is regulated by FUS. Using RNAi overexpression wild-type ALS-associated mutants,...
Report21 March 2011Open Access miRNAs as serum biomarkers for Duchenne muscular dystrophy Davide Cacchiarelli Department of Biology and Biotechnology, Institute Pasteur Cenci-Bolognetti IBPM, Sapienza University Rome, Italy Search more papers by this author Ivano Legnini Julie Martone Valentina Cazzella Adele D'Amico Lab Molecular Medicine, Neuroscience, Bambino Gesù Children's Hospital, Enrico Bertini Irene Bozzoni Corresponding Author [email protected] Information Cacchiarelli1, Legnini1,...
Detailed knowledge of the molecular biology severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is crucial for understanding viral replication, host responses, and disease progression. Here, we report gene expression profiles three SARS-CoV- SARS-CoV-2-infected human cell lines. SARS-CoV-2 elicited an approximately two-fold higher stimulation innate immune response compared to SARS-CoV in epithelial line Calu-3, including induction miRNA-155. Single-cell RNA sequencing...
The muscle-specific long noncoding RNA linc-MD1 was shown to be expressed during early phases of muscle differentiation and trigger the switch later stages by acting as a sponge for miR-133 miR-135. Notably, is also host transcript miR-133b, their biogenesis mutually exclusive. Here, we describe that this alternative synthesis controlled HuR protein, which favors accumulation through its ability bind repress Drosha cleavage. We show under repressive control sponging activity consolidates...
Organoids derived from stem cells have become an increasingly important tool for studying human development and modeling disease. However, methods are still needed to control study spatiotemporal patterns of gene expression in organoids. Here we combined optogenetics perturbation technologies activate or knock-down RNA target genes programmable patterns. To illustrate the usefulness our approach, locally activated Sonic Hedgehog (SHH) signaling organoid model neurodevelopment. Spatial...
Circular RNAs (circRNAs) represent a class of covalently closed RNAs, derived from non-canonical splicing events, which are expressed in all eukaryotes and often conserved among different species. We previously showed that the circRNA originating ZNF609 locus (circ-ZNF609) acts as crucial regulator human primary myoblast growth: indeed, downregulation circRNA, not its linear counterpart, strongly reduced proliferation rate vitro cultured myoblasts. To deepen our knowledge about circ-ZNF609...
Abstract The coronavirus disease 2019 (COVID-19) pandemic, caused by the novel severe acute respiratory syndrome 2 (SARS-CoV-2), is an ongoing global health threat with more than two million infected people since its emergence in late 2019. Detailed knowledge of molecular biology infection indispensable for understanding viral replication, host responses, and progression. We provide gene expression profiles SARS-CoV SARS-CoV-2 infections three human cell lines (H1299, Caco-2 Calu-3 cells),...
Abstract Herpes simplex encephalitis is a life-threatening disease of the central nervous system caused by herpes viruses (HSVs). Following standard care with antiviral acyclovir treatment, most patients still experience various neurological sequelae. Here we characterize HSV-1 infection human brain organoids combining single-cell RNA sequencing, electrophysiology and immunostaining. We observed strong perturbations tissue integrity, neuronal function cellular transcriptomes. Under treatment...
The generation of distinct messenger RNA isoforms through alternative processing modulates the expression and function genes, often in a cell-type-specific manner. Here, we assess regulatory relationships between transcription initiation, splicing, 3′ end site selection. Applying long-read sequencing to accurately represent even longest transcripts from end, quantify mRNA Drosophila tissues, including transcriptionally complex nervous system. We find that heads, as well human cerebral...
// James M. Hughes 1 , Ivano Legnini Beatrice Salvatori 1, 6 Silvia Masciarelli 2 Marcella Marchioni 3 Francesco Fazi Mariangela Morlando Irene Bozzoni 3, 4, 5 Alessandro Fatica Department of Biology and Biotechnology "C. Darwin", Sapienza University Rome, Italy Anatomical, Histological, Forensic & Orthopaedic Sciences, Institute Biology, Molecular Medicine Nanobiotechnology, CNR, 4 Center for Life Nano Science@Sapienza, Istituto Italiano di Tecnologia, Pasteur Fondazione Cenci-Bolognetti,...
Soft-bodied cephalopods such as octopuses are exceptionally intelligent invertebrates with a highly complex nervous system that evolved independently from vertebrates. Because of elevated RNA editing in their tissues, we hypothesized regulation may play major role the cognitive success this group. We thus profiled messenger RNAs and small three cephalopod species including 18 tissues Octopus vulgaris. show innovation soft-bodied is an expansion microRNA (miRNA) gene repertoire. These...
// Pietro Laneve 1 , Agnese Po 2 Annarita Favia 3 Ivano Legnini 4 Vincenzo Alfano 1, Jessica Rea Valerio Di Carlo 4, 11 Valeria Bevilacqua 12 Evelina Miele 13 Angela Mastronuzzi 5 Andrea Carai 6 Franco Locatelli 5, 7 Irene Bozzoni 3, 8 Elisabetta Ferretti 9, 10 Elisa Caffarelli Center for Life NanoScience@Sapienza, Istituto Italiano di Tecnologia, 00161 Rome, Italy Department of Molecular Medicine, Sapienza University Institute Biology and Pathology, National Research Council, 00185...
Abstract Expansion of the glutamine tract (poly-Q) in protein huntingtin (HTT) causes neurodegenerative disorder Huntington’s disease (HD). Emerging evidence suggests that mutant HTT (mHTT) disrupts brain development. To gain mechanistic insights into neurodevelopmental impact human mHTT, we engineered male induced pluripotent stem cells to introduce a biallelic or monoallelic 70Q expansion remove poly-Q HTT. The introduction mutation caused aberrant development cerebral organoids with loss...
// Arianna Mangiavacchi 1, 5, * , Melissa Sorci Silvia Masciarelli 2 Simone Larivera 1 Ivano Legnini Ilaria Iosue Irene Bozzoni 3, 4 Francesco Fazi Alessandro Fatica Department of Biology and Biotechnology "C. Darwiny", Sapienza University Rome, 00185, Italy Anatomical, Histological, Forensic Orthopaedic Sciences, 3 Center for Life Nano Science@Sapienza, Istituto Italiano di Tecnologia, 00161, Institute Pasteur Fondazione Cenci-Bolognetti, 5 Present address: KAUST Environmental Epigenetics...
Circular RNAs (circRNAs) constitute a recently re-discovered class of non-coding functioning as sponges for miRNAs and proteins, affecting RNA splicing regulating transcription. CircRNAs are generated by “back-splicing”, which is the linking covalently 3′- 5′-ends exons. Thus, circRNA levels might be deregulated in conditions associated with altered RNA-splicing. Significantly, growing evidence indicates their role human diseases. Specifically, myotonic dystrophy type 1 (DM1) multisystemic...
ABSTRACT Cells live and interact in three-dimensional (3D) cellular neighborhoods. However, histology spatial omics methods mostly focus on 2D tissue sections. Here we present a 3D atlas of routine clinical sample, an aggressive human lung carcinoma, by combining situ quantification 960 cancer-related genes across ∼340,000 cells with measurements tissue-mechanical components. neighborhoods subdivided the tumor microenvironment into tumor, stromal, immune multicellular niches. Interestingly,...
One promising approach for the gene therapy of Duchenne muscular dystrophy (DMD) is exon skipping. When thinking possible intervention on human, it very crucial to identify most appropriate antisense sequences able provide highest skipping efficiency. In this article, we compared 51 activity 10 different molecules, raised against splice junctions and/or exonic splicing enhancers (ESEs), expressed as part U1 small nuclear RNA (snRNA). The effectiveness each construct was tested in human DMD...