A5086306948- Epigenetics and DNA Methylation
- CRISPR and Genetic Engineering
- Genomics and Chromatin Dynamics
- Immune Cell Function and Interaction
- MicroRNA in disease regulation
- Protein Degradation and Inhibitors
- CAR-T cell therapy research
- Ubiquitin and proteasome pathways
- RNA Interference and Gene Delivery
- Acute Myeloid Leukemia Research
- Immunotherapy and Immune Responses
- Mitochondrial Function and Pathology
- Cancer, Hypoxia, and Metabolism
- T-cell and B-cell Immunology
- Enzyme Structure and Function
- Protein Structure and Dynamics
- Genetics, Bioinformatics, and Biomedical Research
- RNA modifications and cancer
- Transgenic Plants and Applications
- Adenosine and Purinergic Signaling
- Biochemical and Molecular Research
- Autophagy in Disease and Therapy
- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Cancer Immunotherapy and Biomarkers
University of Pennsylvania
2016-2024
Cancer Research Institute
2017-2021
UPMC Hillman Cancer Center
2017-2020
University of Utah
2020
Chinese Academy of Sciences
2018
University of Science and Technology of China
2018
Autophagy regulates stemness Embryonic stem cells can propagate indefinitely and differentiate when called to do so. Xu et al. now analyze how cellular metabolism affects the balance between pluripotency differentiation (see Perspective by Borsa Simon). For in pluripotent state, transcription factors Oct4 Sox2 suppress chaperone-mediated autophagy (CMA). When CMA is released with differentiation, isocitrate dehydrogenases IDH1 IDH2 are degraded, resulting less α-ketoglutarate, which needed...
Abstract Epigenetic programs are dysregulated in acute myeloid leukemia (AML) and help enforce an oncogenic state of differentiation arrest. To identify key epigenetic regulators AML cell fate, we performed a differentiation-focused CRISPR screen cells. This identified the histone acetyltransferase KAT6A as novel regulator that drives critical leukemogenic gene-expression programs. We show is initiator newly described transcriptional control module which KAT6A-catalyzed promoter H3K9ac bound...
MicroRNAs play an important role in T cell responses. However, how microRNAs regulate CD8 memory remains poorly defined. Here, we found that miR-150 negatively regulates vivo. Genetic deletion of disrupted the balance between precursor and terminal effector cells following acute viral infection. Moreover, miR-150-deficient were more protective upon rechallenge. A key circuit whereby repressed development through transcription factor c-Myb was identified. Without miR-150, upregulated...
Metastasizing cancer cells are able to withstand high levels of oxidative stress through mechanisms that poorly understood. Here, we show under various conditions, pancreatic markedly expand NADPH and NADP
RNA polymerase III (Pol III) is responsible for the production of small noncoding species, including tRNAs and 5S rRNA. Pol III-dependent transcription generally enhanced in transformed cells tumors, but underlying mechanisms remain not well-understood. It has been demonstrated that BRF1 subunit TFIIIB essential accurate initiation transcription. However, it known whether undergoes ubiquitin modification ubiquitination regulates Here, we show RNF12, a RING domain-containing E3 ligase,...
Frataxin is the protein that down-regulated in Friedreich ataxia (FRDA), an autosomal recessive genetic disease caused by intronic GAA repeat expansion intron-1 of FXN gene. The repeats result epigenetic silencing gene and reduced expression cytosolic full-length frataxin (1-210) protein. Full length translocates to mitochondria, leading formation mature (81-210) formed cleavage mitochondrial targeting sequence at K-80 There are currently no approved treatments for FRDA, although...
<h3>Background</h3> Chimeric Antigen Receptor (CAR) T cell therapies targeting hematologic malignancies and solid tumors are limited by exhaustion. Exhaustion is induced chronic stimulation through the CAR results in a progressive loss of antitumor function. Stable epigenetic features exhaustion major barrier to conventional reversal approaches like immune checkpoint blockade.<sup>1 2</sup> Thus, there critical unmet need better define epigenome exhausted human cells develop...
Abstract MicroRNAs play an important role in CD8+ T cell differentiation and anti-viral immune responses. However, how microRNAs regulate memory formation remains poorly defined. Here, by microRNA profiling of during acute or chronic LCMV infection, we identified that are specifically enriched cells comparing to naïve, effector exhausted cells. MiR-150, a has been shown be multiple biological processes, was as potential biased microRNA. Functional interrogation the miR-150 demonstrated...
<div>Abstract<p>Epigenetic programs are dysregulated in acute myeloid leukemia (AML) and help enforce an oncogenic state of differentiation arrest. To identify key epigenetic regulators AML cell fate, we performed a differentiation-focused CRISPR screen cells. This identified the histone acetyltransferase KAT6A as novel regulator that drives critical leukemogenic gene-expression programs. We show is initiator newly described transcriptional control module which KAT6A-catalyzed...
Supplementary Data from KAT6A and ENL Form an Epigenetic Transcriptional Control Module to Drive Critical Leukemogenic Gene-Expression Programs
Supplementary Data from KAT6A and ENL Form an Epigenetic Transcriptional Control Module to Drive Critical Leukemogenic Gene-Expression Programs
Supplementary Data from KAT6A and ENL Form an Epigenetic Transcriptional Control Module to Drive Critical Leukemogenic Gene-Expression Programs
Supplementary Data from KAT6A and ENL Form an Epigenetic Transcriptional Control Module to Drive Critical Leukemogenic Gene-Expression Programs
Supplementary Data from KAT6A and ENL Form an Epigenetic Transcriptional Control Module to Drive Critical Leukemogenic Gene-Expression Programs
Supplementary Data from KAT6A and ENL Form an Epigenetic Transcriptional Control Module to Drive Critical Leukemogenic Gene-Expression Programs
<div>Abstract<p>Epigenetic programs are dysregulated in acute myeloid leukemia (AML) and help enforce an oncogenic state of differentiation arrest. To identify key epigenetic regulators AML cell fate, we performed a differentiation-focused CRISPR screen cells. This identified the histone acetyltransferase KAT6A as novel regulator that drives critical leukemogenic gene-expression programs. We show is initiator newly described transcriptional control module which KAT6A-catalyzed...
Supplementary Data from KAT6A and ENL Form an Epigenetic Transcriptional Control Module to Drive Critical Leukemogenic Gene-Expression Programs
Supplementary Data from KAT6A and ENL Form an Epigenetic Transcriptional Control Module to Drive Critical Leukemogenic Gene-Expression Programs
Supplementary Data from KAT6A and ENL Form an Epigenetic Transcriptional Control Module to Drive Critical Leukemogenic Gene-Expression Programs
Supplementary Data from KAT6A and ENL Form an Epigenetic Transcriptional Control Module to Drive Critical Leukemogenic Gene-Expression Programs
Supplementary Data from KAT6A and ENL Form an Epigenetic Transcriptional Control Module to Drive Critical Leukemogenic Gene-Expression Programs
Supplementary Data from KAT6A and ENL Form an Epigenetic Transcriptional Control Module to Drive Critical Leukemogenic Gene-Expression Programs