Derek A. Oldridge
A5052479584- Neuroblastoma Research and Treatments
- Cancer Genomics and Diagnostics
- COVID-19 Clinical Research Studies
- Cancer, Hypoxia, and Metabolism
- SARS-CoV-2 and COVID-19 Research
- Glioma Diagnosis and Treatment
- CAR-T cell therapy research
- Immune Cell Function and Interaction
- Single-cell and spatial transcriptomics
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Cancer-related molecular mechanisms research
- Peroxisome Proliferator-Activated Receptors
- Immune cells in cancer
- Receptor Mechanisms and Signaling
- Radiomics and Machine Learning in Medical Imaging
- Genomic variations and chromosomal abnormalities
- Cardiac electrophysiology and arrhythmias
- Genomics and Rare Diseases
- Hormonal and reproductive studies
- RNA Interference and Gene Delivery
- Epigenetics and DNA Methylation
- Kawasaki Disease and Coronary Complications
- Lung Cancer Research Studies
- Protein Degradation and Inhibitors
University of Pennsylvania
2016-2025
Children's Hospital of Philadelphia
2016-2025
Cornell University
2010-2023
Philadelphia University
2021-2022
Translational Therapeutics (United States)
2020-2022
University of California, San Francisco
2019
University of Naples Federico II
2014
Abramson Cancer Center
2014
Ceinge Biotecnologie Avanzate (Italy)
2014
Sapienza University of Rome
2014
Coronavirus disease 2019 (COVID-19) is currently a global pandemic, but human immune responses to the virus remain poorly understood. We used high-dimensional cytometry analyze 125 COVID-19 patients and compare them with recovered healthy individuals. Integrated analysis of ~200 ~50 clinical features revealed activation T cell B subsets in proportion patients. A subgroup had characteristic acute viral infection plasmablast reaching >30% circulating cells. However, another lymphocyte...
The CD19 antigen, expressed on most B-cell acute lymphoblastic leukemias (B-ALL), can be targeted with chimeric antigen receptor-armed T cells (CART-19), but relapses epitope loss occur in 10% to 20% of pediatric responders. We detected hemizygous deletions spanning the locus and de novo frameshift missense mutations exon 2 some relapse samples. However, we also discovered alternatively spliced mRNA species, including one lacking 2. Pull-down/siRNA experiments identified SRSF3 as a splicing...
Immune memory after vaccination Vaccination against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has proven highly effective at preventing COVID-19. However, the evolution of viral variants, and waning antibody levels over time, raise questions regarding longevity vaccine-induced immune protection. Goel et al . examined B T lymphocyte responses in individuals who received SARS-CoV-2 messenger RNA vaccines. They performed a 6-month longitudinal study never had infection...
Novel mRNA vaccines for SARS-CoV-2 have been authorized emergency use. Despite their efficacy in clinical trials, data on vaccine-induced immune responses are mostly limited to serological analyses. Here, we interrogated antibody and antigen-specific memory B cells over time 33 naïve 11 recovered subjects. individuals required both vaccine doses optimal increases antibodies, particularly neutralizing titers against the B.1.351 variant. Memory specific full-length spike protein receptor...
Patients with cancer have high mortality from coronavirus disease 2019 (COVID-19), and the immune parameters that dictate clinical outcomes remain unknown. In a cohort of 100 patients who were hospitalized for COVID-19, hematologic had higher relative to solid cancer. two additional cohorts, flow cytometric serologic analyses demonstrated without similar phenotype during acute whereas impairment B cells severe respiratory syndrome 2 (SARS-CoV-2)-specific antibody responses. Despite impaired...
Previous studies have shown that MR imaging features can be used to predict survival and molecular profile of glioblastoma. However, no study a similar type has been performed on lower-grade gliomas (LGGs).Presurgical MRIs 165 patients with diffuse low- intermediate-grade (histological grades II III) were scored according the Visually Accessible Rembrandt Images (VASARI) annotations. Radiomic models using automated texture analysis VASARI built isocitrate dehydrogenase 1 (IDH1) mutation,...
Pediatric COVID-19 following SARS-CoV-2 infection is associated with fewer hospitalizations and often milder disease than in adults. A subset of children, however, present Multisystem Inflammatory Syndrome Children (MIS-C) that can lead to vascular complications shock, but rarely death. The immune features MIS-C compared pediatric or adult remain poorly understood. We analyzed peripheral blood responses hospitalized infected patients (pediatric COVID-19) MIS-C. had patterns T cell-biased...
Tisagenlecleucel, a chimeric antigen receptor (CAR) T-cell product targeting CD19 is approved for relapsed/refractory B-cell acute lymphoblastic leukemia (B-ALL). However, the impact of pretreatment variables, such as expression level, on leukemic blasts, presence CD19- subpopulations, and especially prior CD19-targeted therapy, response to CAR therapy has not been determined. We analyzed 166 patients treated with at our institution. Eleven did achieve minimal residual disease (MRD)- deep...
Viral infection of the respiratory tract can be associated with propagating effects on airway microbiome, and microbiome dysbiosis may influence viral disease. Here, we investigated in coronavirus disease 2019 (COVID-19) its relationship to severity, systemic immunologic features, outcomes. We examined 507 oropharyngeal, nasopharyngeal, endotracheal samples from 83 hospitalized COVID-19 patients as well non-COVID healthy controls. Bacterial communities were interrogated using 16S rRNA gene...
Non-hematopoietic cells are essential contributors to hematopoiesis. However, heterogeneity and spatial organization of these in human bone marrow remain largely uncharacterized. We used single-cell RNA sequencing (scRNA-seq) profile 29,325 non-hematopoietic discovered nine transcriptionally distinct subtypes. simultaneously profiled 53,417 hematopoietic predicted their interactions with subsets. employed co-detection by indexing (CODEX) spatially over 1.2 million cells. integrated scRNA-seq...
Abstract Chromosome 6p22 was identified recently as a neuroblastoma susceptibility locus, but its mechanistic contributions to tumorigenesis are yet undefined. Here we report that the most highly significant single-nucleotide polymorphism (SNP) associations reside within CASC15, long noncoding RNA define tumor suppressor at 6p22. Low-level expression of short CASC15 isoform (CASC15-S) associated with advanced and poor patient survival. In human cells, attenuating CASC15-S increased cellular...
Abstract High-risk neuroblastomas show a paucity of recurrent somatic mutations at diagnosis. As result, the molecular basis for this aggressive phenotype remains elusive. Recent progress in regulatory network analysis helped us elucidate disease-driving mechanisms downstream genomic alterations, including chromosomal alterations. Our identified three subtypes high-risk neuroblastomas, consistent with and subtype-specific master regulator proteins that were conserved across independent...
High-throughput sequencing of DNA coding regions has become a common way assaying genomic variation in the study human diseases. Copy number (CNV) is an important type variation, but detecting and characterizing CNV from exome challenging due to high level biases artifacts. We propose CODEX, normalization calling procedure for whole data. The Poisson latent factor model CODEX includes terms that specifically remove GC content, exon capture amplification efficiency, systemic also...
TP53 is the most frequently mutated gene in human malignancies; however, de novo somatic mutations childhood embryonal cancers such as neuroblastoma are rare. We report on analysis of three independent case-control cohorts comprising 10290 individuals and demonstrate that rs78378222 rs35850753, rare germline variants linkage disequilibrium map to 3' untranslated region (UTR) 5' UTR Δ133 isoform TP53, respectively, robustly associated with (rs35850753: odds ratio [OR] = 2.7, 95% confidence...
Abstract COVID-19 has become a global pandemic. Immune dysregulation been implicated, but immune responses remain poorly understood. We analyzed 71 patients compared to recovered and healthy subjects using high dimensional cytometry. Integrated analysis of ∼200 >30 clinical features revealed activation T cell B subsets, only in some patients. A subgroup had characteristic acute viral infection plasmablast could reach >30% circulating cells. However, another lymphocyte comparable...