A5036963504- T-cell and B-cell Immunology
- Metabolism, Diabetes, and Cancer
- Immune Cell Function and Interaction
- Cancer Genomics and Diagnostics
- Single-cell and spatial transcriptomics
- Acute Myeloid Leukemia Research
- Adipose Tissue and Metabolism
- Sirtuins and Resveratrol in Medicine
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Ubiquitin and proteasome pathways
- CAR-T cell therapy research
- Cellular transport and secretion
- Erythrocyte Function and Pathophysiology
- Multiple Myeloma Research and Treatments
- Cancer Cells and Metastasis
- COVID-19 Clinical Research Studies
- Muscle metabolism and nutrition
- Platelet Disorders and Treatments
- Advanced Biosensing Techniques and Applications
- Chronic Lymphocytic Leukemia Research
- Electrolyte and hormonal disorders
- Molecular Biology Techniques and Applications
- Cancer-related gene regulation
- Pancreatic function and diabetes
- Ion Transport and Channel Regulation
University of Pennsylvania
2021-2025
Translational Therapeutics (United States)
2022-2023
Hospital of the University of Pennsylvania
2019
Yale University
2012-2015
Staten Island University Hospital
2015
Institute of Cell Biology
2015
Patients with COVID-19 present a wide variety of clinical manifestations. Thromboembolic events constitute significant cause morbidity and mortality in patients infected SARS-CoV-2. Severe has been associated hyperinflammation pre-existing cardiovascular disease. Platelets are important mediators sensors inflammation directly affected by stressors. In this report, we found that platelets from severely ill, hospitalized exhibited higher basal levels activation measured P-selectin surface...
The mechanisms by which FOXP3+ T follicular regulatory (Tfr) cells simultaneously steer antibody formation toward microbe or vaccine recognition and away from self-reactivity remain incompletely understood. To explore underappreciated heterogeneity in human Tfr cell development, function, localization, we used paired TCRVA/TCRVB sequencing to distinguish tonsillar that are clonally related natural (nTfr) those likely induced helper (Tfh) (iTfr). proteins iTfr nTfr differentially expressed...
Insulin causes the exocytic translocation of GLUT4 glucose transporters to stimulate uptake in fat and muscle. Previous results support a model which TUG traps intracellular, insulin-responsive vesicles termed storage (GSVs). triggers cleavage release GSVs; then recycles through endosomes during ongoing insulin exposure. The C terminus binds GSV anchoring site comprising Golgin-160 possibly other proteins. Here, we report that is acetylated. C-terminal peptide bound Golgin-160-associated...
Mutations found in AML such as DNMT3A, TET2 and ASXL1 can be the peripheral blood of healthy adults - a phenomenon termed clonal hematopoiesis (CH). These mutations are thought to represent earliest genetic events evolution AML. Genomic studies on samples acquired at diagnosis, remission, relapse have demonstrated significant stability CH following induction chemotherapy. Meanwhile, later genes NPM1 FLT3, been shown contract remission case FLT3 often absent relapse. We sought understand how...
To promote glucose uptake into fat and muscle cells, insulin causes the translocation of GLUT4 transporters from intracellular vesicles to cell surface. Previous data support a model in which TUG traps GLUT4-containing tethers them intracellularly unstimulated cells mobilizes this pool by releasing tether. Here we show that undergoes site-specific endoproteolytic cleavage, separates GLUT4-binding, N-terminal region C-terminal previously suggested bind an anchor. Cleavage is accelerated...
Abstract Rewiring exhausted CD8 T cells (T EX ) towards more functional states is a major goal of cancer immunotherapy but has proven challenging due to the epigenetic stability . Indeed, are epigenetically programmed by transcription factor Tox. However, changes continue occur as transition from progenitor prog ), intermediate int and terminal term subsets, suggesting potential developmental flexibility in mature subsets. By examining into cells, we discovered reciprocally antagonistic...
Hyperglycemia has been shown to influence fluorine-18 fluorodeoxyglucose ((18)F-FDG) uptake in tumor cells. Therefore, patients are instructed fast for 6 h, while maintaining serum glucose levels at an acceptable range. The study was performed evaluate the effect of fasting blood on biodistribution (18)F-FDG various tissues including liver, heart, bone marrow, skeletal muscle, and tumors.Fingerstick is routinely measured morning procedure. maximum standardized value (SUV(max)) right left...
Transformation of follicular lymphoma (FL) into B-lymphoblastic leukemia/lymphoma (B-ALL/LBL) is rare and results in greatly increased aggressiveness clinical course. Here we present extensive molecular analysis this unusual transformation, including immunoglobulin (Ig) gene rearrangement studies, cytogenetic analysis, whole-exome sequencing (WES) the patient's FL, B-ALL/LBL, normal cells. Although FL showed marked somatic hypermutation (SHM) Ig genes, SHM appeared to be even more B-ALL/LBL....
ABSTRACT Mutations commonly found in AML such as DNMT3A , TET2 and ASXL1 can be the peripheral blood of otherwise healthy adults – a phenomenon referred to clonal hematopoiesis (CH). These mutations are thought represent earliest genetic events evolution AML. Genomic studies on samples acquired at diagnosis, remission, relapse have demonstrated significant stability CH following induction chemotherapy. Meanwhile, later genes NPM1 FLT3 been shown contract remission case often absent relapse....
Abstract How FOXP3 + T follicular regulatory (Tfr) cells simultaneously steer antibody formation toward microbe/vaccine recognition and away from self-reactivity remains unsettled. To explore human Tfr cell provenance, function location heterogeneity, we used paired TCRVA / TCRVB sequencing to distinguish tonsillar clonally related natural Tregs (nTfr) those likely induced Tfh (iTfr). The proteins iTfr nTfr differentially expressed were utilized pinpoint their in situ locations via...
Abstract The tumor microenvironment (TME) plays important roles in progression, therapy response and patient survival. In this comprehensive study, we performed a detailed multiomic single cell analysis of breast cancers with inherited mutations different genes (BRCA1, BRCA2, PALB2, ATM, CHEK2), wild type mutation negative (WT) patients, stratified by hormone receptor status, to understand the relationship between immunogenicity. Using spatial tissue multiplexing (PhenoCycler), RNAseq (10X...
Abstract Breast cancer type 1 and 2 susceptibility proteins (BRCA1/BRCA2) are well known breast genes, mutations in which lead to defective homologous recombination repair (HRR). HR-based DNA deficiency (HRD) scores can be used indicate damage, genomic instability may predict response damaging agents BRCA1/2 mutated tumors. Tumors with a high HRD score caused by complete loss of BRCA1 or BRCA2 function locus-specific LOH sensitive damage including platinum-based chemotherapy poly...