A5019632307- Nanoparticle-Based Drug Delivery
- Extracellular vesicles in disease
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Nanoplatforms for cancer theranostics
- Nanoparticles: synthesis and applications
- Graphene and Nanomaterials Applications
- Metal complexes synthesis and properties
- Chromatin Remodeling and Cancer
- Erythrocyte Function and Pathophysiology
- Viral-associated cancers and disorders
- Polymer Nanocomposite Synthesis and Irradiation
- Phytochemicals and Antioxidant Activities
- Reproductive System and Pregnancy
- Characterization and Applications of Magnetic Nanoparticles
- Trace Elements in Health
- Blood properties and coagulation
- Electrospun Nanofibers in Biomedical Applications
- Plant Micronutrient Interactions and Effects
- Microfluidic and Bio-sensing Technologies
- Horticultural and Viticultural Research
- Fermentation and Sensory Analysis
- Micro and Nano Robotics
- Advanced biosensing and bioanalysis techniques
- Infectious Aortic and Vascular Conditions
University of Kansas Medical Center
2023-2025
Kansas State University
2019-2022
Kathmandu University
2016-2018
Novel magnetic composite nanoparticles (MCPs) were successfully synthesized by ex situ conjugation of ZnO (ZnO NPs) and Fe3O4 NPs using trisodium citrate as linker with an aim to retain key properties both viz. inherent selectivity towards cancerous cell superparamagnetic nature, respectively, on a single system. Successful characterization was done XRD, TEM, FTIR, VSM analyses. analysis showed similar profile thus obtained MCPs that naked reduction in saturation magnetization 16.63 emu/g....
Nanoparticles (NPs) are receiving increasing interest in biomedical research owing to their comparable size with biomolecules, novel properties and easy surface engineering for targeted therapy, drug delivery selective treatment making them a better substituent against traditional therapeutic agents. ZnO NPs, despite other applications, also show anticancer property which makes it good option over metal oxide NPs. NPs were synthesized by chemical precipitation technique, then modified using...
The human fallopian tube epithelium (hFTE) is the site of fertilization, early embryo development, and origin most high-grade serous ovarian cancers (HGSOCs). Little known about content functions hFTE-derived small extracellular vesicles (sEVs) due to limitations biomaterials proper culture methods. We have established a microfluidic platform hFTE for EV collection with adequate yield mass spectrometry-based proteomic profiling, reported 295 common sEV proteins first time. These are...
Long interspersed element 1 (LINE-1) retrotransposons are repetitive sequences that can move within the genome by an autonomous mechanism. To limit their mutagenic potential, benign cells restrict LINE-1 expression through molecular mechanisms such as DNA methylation and histone modification, but these usually impaired in cancer. Clear cell ovarian carcinoma (CCOC) represents 5-10% of cancers is thought to arise from endometriosis. Women with advanced CCOC face poor prognoses, highlighting...
The fallopian tube (FT) plays a crucial role in fertility, gynecological health, and high-grade serous ovarian cancer (HGSOC) development. Despite its importance, the spatial transcriptome of FT's distinct anatomical regions (fimbria, infundibulum, ampulla, isthmus) remains underexplored. Using GeoMx Digital Spatial Profiler (DSP) targeted ~1800 gene panel, we analyze premenopausal FT epithelium, identifying region-specific expression patterns. Our analysis reveals upregulation mature...
Reconstruction of extracellular vesicles with imaging agents allows precise downstream analysis using clinical modalities, for example, MRI. This will further improve the biocompatibility thereby enhancing investigations.
Recent progress in bioimaging nanotechnology has a great impact on the diagnosis, treatment, and prevention of diseases by enabling early intervention. Among different types modalities, contrast-enhanced magnetic resonance imaging using paramagnetic gadolinium-based molecular contrast agents (GBCAs) are most commonly used clinic. However, GBCAs distribute rapidly between plasma interstitial spaces with short half-lives limiting its clinical impacts. To improve properties GBCAs, herein an...
Abstract High grade serous ovarian carcinoma (HGSOC) accounts for ~ 70% of cancer cases. Non-invasive, highly specific blood-based tests pre-symptomatic screening in women are crucial to reducing the mortality associated with this disease. Since most HGSOCs typically arise from fallopian tubes (FT), our biomarker search focused on proteins found surface extracellular vesicles (EVs) released by both FT and HGSOC tissue explants representative cell lines. Using mass spectrometry, 985 EV...
A simple and versatile nanoformulation strategy is presented by combining the synthetic lipids, biocompatible polymer, tumor penetrating peptide (LyP-1) into a composite nanosystem for targeted drug delivery imaging in single session.
Red blood cells (RBCs) make up the overwhelming majority of in vascular system, spending most their lives wandering vast network vessels that permeate every tissue our bodies. Therefore, delivery any class therapeutic agent must stay circulatory system may benefit from being carried by RBCs. Toward this direction, we have re-engineered a synthetic liposome with membranes RBCs and incorporated magnetic resonance imaging (MRI) contrast gadolinium along chemotherapeutic drug doxorubicin (DOX)...
Magnetic resonance imaging (MRI) is a routinely used technique in medical diagnostics. To enhance the quality of MR images, contrast agents (CAs) are used, which account for nearly 40% MRI exams clinic globally. The most CAs gadolinium-based (GBCAs) but use GBCAs has been linked with metal-deposition vital organs. Gadolinium deposition shown to be correlated nephrogenic systemic fibrosis, fibrosis skin and internal Therefore, there an unmet need new CA alternative T1-weighted Ce-MRI. Herein,...
Abstract We evaluated the quality of wines produced in Nepal terms phenolic, flavonoid, anthocyanin and tannin content, antioxidant capacity, color parameters using spectrophotometric methods. The total phenolic flavonoid activities Nepali ranged from 85.5 to 960.0 (mean = 360.5 ± 268.7) mg/L GAE , 40.9–551.3 188.9 161.5) QE 66.6–905.0 332.8 296.5) AAE respectively. These were significantly higher red compared white wines. content showed strong correlation with each other as well activities....
Supercritical carbon dioxide–assisted synthesis of poly(N-isopropylacrylamide)–ferrite nanocomposites was carried out by polymerization reaction N-isopropyl acrylamide monomer in the presence ferrite nanoparticles. They were characterized Fourier transform infrared, X-ray diffraction, transmission electron microscopy, atomic force and vibrating sample magnetometry analysis. Drug loading release profiles studied. Nanomaterials showed pH-dependent drug profile. Polymer comparison to...
Abstract FePO 4 NPs are of special interest in food fortification and biomedical imaging because their biocompatibility, high bioavailability, magnetic property, superior sensory performance that do not cause adverse organoleptic effects. These characteristics desirable drug delivery as well. Here, we explored the nanoparticles a vehicle for anticancer drug, doxorubicin, with an optimum loading 26.81% ± 1.0%. This further enforces formation Fe 3+ doxorubicin complex resulting -DOX...
The aim of this study was to investigate the distribution, tolerance, and anticancer antiviral activity Zn-based physiometacomposites (PMCs). Manganese, iron, nickel cobalt-doped ZnO, ZnS or ZnSe were synthesized. Cell uptake, distribution into 3D culture mice, biochemical chemotherapeutic studied by fluorescence/bioluminescence, confocal microscopy, flow cytometry, viability, antitumor virus titer assays. Luminescence inductively coupled plasma mass spectrometry analysis showed that...
Abstract Epithelial ovarian cancer (EOC) remains the leading cause of death from gynecological malignancy and fifth all cancer-related deaths among American women. The most common lethal subtype, high-grade serous (HGSOC) tends to originate fallopian tube. Precursor lesions HGSOC such as secretory cell expansion (SCE), loss PAX2, PTEN, p53 mutation tubal intraepithelial carcinoma (STIC) are often found at tube epithelium (FTE). However, mechanism how neoplastic disseminate in FTE eventually...
Extracellular vesicles (EVs) are cell-secreted lipid bilayer delimited particles that mediate cellular communication. These tiny sacs of information play an important role in cell communication and alter the physiological process under both normal pathological conditions. As such, tracking EVs can provide valuable regarding basic understanding communication, onset early malignancy, biomarker discovery. Most current EV-tracking strategies invasive, altering natural characteristics by...
Abstract Background: Liquid biopsy offers a non-invasive method for detection of non-Hodgkin lymphoma (NHL). Tissue biopsy, the standard NHL detection, causes patient discomfort and increases risk infection. Extracellular vesicles (EVs) are secreted by cells as particles with cargos nucleic acids proteins enclosed in lipid bilayer. The most common subtype is diffuse large B-cell (DLBCL). We analyzed plasma-derived EVs from DLBCL patients matching healthy controls microRNA (miRNA) protein to...