A5062480782- Extracellular vesicles in disease
- MicroRNA in disease regulation
- Ovarian cancer diagnosis and treatment
- Cardiovascular Disease and Adiposity
- RNA Interference and Gene Delivery
- Cytokine Signaling Pathways and Interactions
- Virus-based gene therapy research
- RNA Research and Splicing
- Cell Adhesion Molecules Research
- Animal Virus Infections Studies
- Circular RNAs in diseases
- Ultrasound and Hyperthermia Applications
- Caveolin-1 and cellular processes
- Cancer-related molecular mechanisms research
- Cancer, Lipids, and Metabolism
- Photoacoustic and Ultrasonic Imaging
- PI3K/AKT/mTOR signaling in cancer
- Adipokines, Inflammation, and Metabolic Diseases
- RNA modifications and cancer
- Lattice Boltzmann Simulation Studies
- Ferroptosis and cancer prognosis
- Galectins and Cancer Biology
- Ultrasound Imaging and Elastography
- Immune Cell Function and Interaction
- Reproductive System and Pregnancy
The Ohio State University Comprehensive Cancer Center – Arthur G. James Cancer Hospital and Richard J. Solove Research Institute
2024
The Ohio State University Wexner Medical Center
2016-2024
The Ohio State University
2016-2024
Kurume University
2024
U-M Rogel Cancer Center
2018
Comprehensive Blood & Cancer Center
2018
Ohio University
2017
Exosomes are nanoscale vesicles found in many bodily fluids which play a significant role cell-to-cell signaling and contain biomolecules indicative of their cells origin. Recently, microfluidic devices have provided the ability to efficiently capture exosomes based on specific membrane biomarkers, but releasing captured intact label-free for downstream characterization experimentation remains challenge. We present herringbone-grooved device is covalently functionalized with antibodies...
Abstract Because of limits on specificity and purity to allow for in-depth protein profiling, a standardized method exosome isolation has yet be established. In this study, we describe novel, in-house microfluidic-based device isolate exosomes from culture media patient samples. This technology overcomes contamination issues because sample separation is based the expression highly specific surface markers CD63 EpCAM. Mass spectrometry revealed over 25 proteins that are differentially...
STAT3 is over-expressed in endometrial cancer, and diabetes a risk factor for the development of type 1 cancer. We therefore investigated whether glucose concentrations influence expression such might be inhibited by metformin.In Ishikawa (grade 1) cancer cells subjected to media with low, normal, or high glucose, its target proteins was evaluated real-time quantitative PCR (qPCR). were treated metformin assessed cell proliferation, survival, migration, ubiquitin assays, as well Western blot...
Advanced ovarian clear cell carcinoma (OCCC) carries a very poor prognosis in large part secondary to the extremely high rate of resistance standard platinum and taxane chemotherapy. Signal transducer activator transcription 3(STAT3) expression activation has been shown regulate tumor progression various human cancers, though not well studied OCCC. Preliminary work our lab demonstrated constitutive STAT3 (pSTAT3Tyr705 or pSTAT3727) OCCC lines as tissue samples. Significantly, pSTAT3 is...
Abstract The interferon stimulated gene 15 (ISG15), a ubiquitin like protein and its conjugates have been implicated in various human malignancies. However, role ovarian cancer progression metastasis is largely unknown. In high grade serous (HGSOC), ascites the major contributor to peritoneal metastasis. this study, we identified significantly elevated ISG15 expression HGSOC patient ascites, derived primary cells (POCCs), POCC small extracellular vesicles (sEVs) as well metastatic tissue....
Abstract Introduction: High-grade serous ovarian cancer (HGSOC) accounts for over 75% of all epithelial and has a high mortality rate due to lack early detection methods. Many biomarkers have been proposed; however, none shown improve at an stage in this patient population. The purpose study is assess whether there are unique extracellular vesicle (EV) protein signatures early-stage HGSOC that could serve as biomarkers. Methods: 250 serum samples were obtained from multi-institutional...
Ovarian clear cell carcinoma (OCCC) accounts for approximately 8-10% of epithelial ovarian cancers in the United States. Although it is rare, OCCC usually presents with treatment challenges and overall prognosis far worse than high grade serous cancer HGSOC. The objective this study was to examine therapeutic relevance combining oncolytic virus cisplatin (OCCC).We identified that TMEM205, a recently discovered transmembrane protein, contributes chemoresistance cells via exosomal pathway....
Abstract The role of extracellular vesicles (EVs) in human health and disease has garnered considerable attention over the past two decades. However, while several types EVs are known to interact dynamically with matrix there is great potential value producing high-fidelity EV micropatterns, currently no label-free, high-resolution, tunable platform technologies this capability. We introduce Light-induced Extracellular Vesicle Adsorption (LEVA) as a powerful solution rapidly advance study...
Abstract Endometrial cancer (EC) is the leading gynecologic malignancy in United States with obesity implicated 57% of cases. This research delves into molecular complexities extracellular vesicle (EV) secretion as carriers oncogenic protein expression and their involvement obesity-mediated EC. An understanding these mechanisms pivotal for unraveling pathways relevant to obesity-associated EC, thereby guiding development innovative prevention treatment strategies. Our investigation revealed...
Abstract Introduction: Endometrial cancer (EC) is the leading gynecologic malignancy in U.S., with obesity implicated 57% of cases. This study explores molecular workings oncogenic protein expression (TMEM205, STAT5, and FAS) their role regulating exosome secretion. Understanding these mechanisms crucial for unraveling pathways involved obesity-related EC, informing development innovative strategies prevention treatment. Methods: The isolated exosomes were quantified using nanoparticle...
Abstract Endometrial cancer (EC) is the leading gynecologic malignancy in United States with obesity implicated 57% of cases. This research investigates molecular complexities extracellular vesicles (EV) secretion as carriers oncogenic protein and their involvement obesity-mediated EC. An understanding these mechanisms pivotal for unraveling pathways relevant to obesity-associated EC, thereby guiding development innovative prevention treatment strategies. Our exploration revealed a...
<div>Abstract<p>Because of limits on specificity and purity to allow for in-depth protein profiling, a standardized method exosome isolation has yet be established. In this study, we describe novel, in-house microfluidic-based device isolate exosomes from culture media patient samples. This technology overcomes contamination issues because sample separation is based the expression highly specific surface markers CD63 EpCAM. Mass spectrometry revealed over 25 proteins that are...
<p>Supplementory Materials and Methods for Microfluidic chip based exosome proteomic profiling</p>
<p>Supplementary Figures: S1 is a work flow for exosome isolation in cancer cells. S2-S5 protomic profiling cells Vs normal using different techniques. S6-S8 The top Canonical pathways identified the protein</p>
<p>Supplementary Figures: S1 is a work flow for exosome isolation in cancer cells. S2-S5 protomic profiling cells Vs normal using different techniques. S6-S8 The top Canonical pathways identified the protein</p>
<p>Sup. Tables: S1-S3 is Gene Ontology Term analysis by Panther db in cancer cells Vs normal cells. S4-S6 IPA core showing the top protein molecules identified to be upregulated S7 Proximity Extension Assay for select Immuno-oncology panel proteins that were significantly higher HGSOC</p>
<p>Supplementory Materials and Methods for Microfluidic chip based exosome proteomic profiling</p>