A5057132906- Hemoglobin structure and function
- Erythrocyte Function and Pathophysiology
- Heme Oxygenase-1 and Carbon Monoxide
- Hemoglobinopathies and Related Disorders
- Neonatal Health and Biochemistry
- Pancreatic function and diabetes
- Neuroscience of respiration and sleep
- Nitric Oxide and Endothelin Effects
- Cardiac Arrest and Resuscitation
- Blood properties and coagulation
- Extracellular vesicles in disease
- Iron Metabolism and Disorders
- Blood transfusion and management
- Cancer, Hypoxia, and Metabolism
- Organ Transplantation Techniques and Outcomes
- High Altitude and Hypoxia
- Traumatic Brain Injury and Neurovascular Disturbances
- Mechanical Circulatory Support Devices
- Polymer Surface Interaction Studies
- Microfluidic and Bio-sensing Technologies
- Nanoplatforms for cancer theranostics
- Transplantation: Methods and Outcomes
- Cellular Mechanics and Interactions
- Protein Structure and Dynamics
- Trauma, Hemostasis, Coagulopathy, Resuscitation
The Ohio State University
2016-2025
University of California, San Diego
1999-2021
The Ohio State University Comprehensive Cancer Center – Arthur G. James Cancer Hospital and Richard J. Solove Research Institute
2015
Veterinary Medical Center
2015
University Hospital of Zurich
2015
Emory University
2013
Bloodworks Northwest
2013
Institute for Transfusion Medicine
2013
University of Pittsburgh
2013
National Heart Lung and Blood Institute
2008-2011
Filamentous actin (F-actin), one of the constituents cytoskeleton, is believed to be most important participant in motion and mechanical integrity eukaryotic cells. Traditionally, viscoelastic moduli F-actin networks have been measured by imposing a small strain quantifying resulting stress. The magnitude moduli, their concentration dependence dependence, as well nature (solid-like or liquid-like) uncross-linked F-actin, subjects debate. Although this paper helps resolve debate establishes...
Bovine hemoglobin (Hb) was encapsulated inside polymer vesicles (polymersomes) to form polymersome Hb (PEH) dispersions. PEH particles are 100% surface PEGylated with longer PEG chains and possess thicker hydrophobic membranes as compared conventional liposomes. Polymersomes were self-assembled from poly(butadiene)-poly(ethylene glycol) (PBD-PEO) amphiphilic diblock copolymers PBD-PEO molecular weights of 22−12.6, 5−2.3, 2.5−1.3, 1.8−0.9 kDa. The first two possessed linear PBD blocks, while...
This work describes the development of polymersome-encapsulated hemoglobin (PEH) self-assembled from biodegradable and biocompatible amphiphilic diblock copolymers composed poly(ethylene oxide) (PEO), poly(caprolactone) (PCL), poly(lactide) (PLA). In diblock, PEO functions as hydrophilic block, while either PCL or PLA can function hydrophobic block. PEO, PCL, are polymers, last two polymers biodegradable. PEH dispersions were prepared by extrusion through 100 nm pore radii polycarbonate...
Neuroinflammation is a well-recognized consequence of subarachnoid hemorrhage (SAH), and may be responsible for important complications SAH. Signaling by Toll-like receptor 4 (TLR4)-mediated nuclear factor κB (NFκB) in microglia plays critical role neuronal damage after Three molecules derived from erythrocyte breakdown have been postulated to endogenous TLR4 ligands: methemoglobin (metHgb), heme hemin. However, poor water solubility hemin, lipopolysaccharide (LPS) contamination confounded...
Rationale: Hemolysis occurs not only in conditions such as sickle cell disease and malaria but also during transfusion of stored blood, extracorporeal circulation, sepsis. Cell-free Hb depletes nitric oxide (NO) the vasculature, causing vasoconstriction eventually cardiovascular complications. We hypothesize that Hb-binding proteins may preserve vascular NO signaling hemolysis.Objectives: Characterization an archetypical function by which scavenger could hemolysis.Methods: investigated...
Exosome size distributions and numbers of exosomes released per cell are measured by asymmetric flow-field flow fractionation/multi-angle light scattering (A4F/MALS) for three thyroid cancer lines as a function treatment that inhibits MAPK signaling pathways in the cells. We show these release with well-defined morphological features reflect common biological process their formation into extracellular environment. find those constitutive activation pathway display MEK-dependent exosome...
Delayed ischemic neurological deficit (DIND) is a major driver of adverse outcomes in patients with aneurysmal subarachnoid hemorrhage (aSAH), defining an unmet need for therapeutic development. Cell-free hemoglobin that released from erythrocytes into the cerebrospinal fluid (CSF) suggested to cause vasoconstriction and neuronal toxicity, correlates occurrence DIND. CSF aSAH disrupted dilatory NO signaling ex vivo cerebral arteries, which shifted vascular tone balance dilation constriction....
Abstract Extracellular vesicles (EVs) derived from tumor cells have the potential to provide a much-needed source of non-invasive molecular biomarkers for liquid biopsies. However, current methods EV isolation limited specificity towards tumor-derived EVs that limit their clinical use. Here, we present an approach called immunomagnetic sequential ultrafiltration (iSUF) consists stages purification and enrichment in approximately 2 h. In iSUF, different volumes biofluids (0.5–100 mL) can be...
Abstract Conventional PD‐L1 immunohistochemical tissue biopsies only predict 20%–40% of non‐small cell lung cancer (NSCLC) patients that will respond positively to anti‐PD‐1/PD‐L1 immunotherapy. Herein, we present an immunogold biochip quantify single extracellular vesicular RNA and protein ( Au SERP) as a non‐invasive alternative. With 20 μl purified serum, PD‐1/PD‐L1 proteins on the surface vesicles (EVs) EV messenger (mRNA) cargo were detected at single‐vesicle resolution exceeded...
The molecular heterogeneity of extracellular vesicles (EVs) and the co-isolation physically similar particles, such as lipoproteins (LPs), confounds limits sensitivity EV bulk biomarker characterization. Herein, we present a single-EV particle (siEVP) protein RNA assay (
We report a systematic study of the linear rheology solutions model semiflexible polymers, actin filaments (F-actin), using mechanical rheometry, diffusing wave spectroscopy (DWS), and video-based single-particle tracking microrheology. For pure at c = 24 μM after full polymerization, elastic loss moduli still increase with time as G'(t) ∝ t0.25±0.02 G''(t) t0.15±0.03, when measured 1 rad/s, during network formation reach plateau 12 h. At equilibrium, small-frequency modulus has small...
In this work, self-assembled poly(butadiene)-b-poly(ethylene oxide) (PB-PEO) polymersomes (polymer vesicles) and worm micelles were evaluated as paclitaxel carriers. Paclitaxel was successfully incorporated into PB-PEO micelles. The loading capacity of inside colloids ranged from 6.7% to 13.7% w/w, depending on the morphology copolymer molecular weight diblock copolymer. loaded OB4 (PB219-PEO121) polymersome formulations colloidally stable for 4 months at degrees C exhibited slow steady...
Abstract Bovine and human hemoglobin (bHb hHb, respectively) was purified from bovine red blood cells via tangential flow filtration (TFF) in four successive stages. TFF is a fast simple method to purify Hb RBCs using through hollow fiber (HF) membranes. Most of the retained stage III (100 kDa HF membrane) displayed methemoglobin levels less than 1%, yielding final concentrations 318 300 mg/mL for bHb respectively. Purified exhibited much lower endotoxin their respective RBCs. The purity...
The storage of red blood cells (RBCs) results in numerous changes, which over time result decreased recovery transfused RBCs. In addition (at least animal models), stored RBCs can be more immunogenic and also stimulate the systemic release inflammatory cytokines transfusion recipients. One component RBC lesion is accumulation oxidative damage. We tested hypothesis that adding a chemical antioxidant (ascorbic acid) to would improve quality RBCs.RBCs were harvested from FVB.HOD mice express an...
In this study, we investigated the size distribution, encapsulation efficiency, and oxygen affinity of liposome-encapsulated tetrameric hemoglobin (LEHb) dispersions correlated data with variation in extruder membrane pore size, ionic strength extrusion buffer, (Hb) concentration. Asymmetric flow field-flow fractionation (AFFF) series multi-angle static light scattering (MASLS) was used to study LEHb distribution. We also introduced a novel method measure efficiency using differential...