A5062556449- Congenital heart defects research
- Single-cell and spatial transcriptomics
- RNA modifications and cancer
- Mathematical Biology Tumor Growth
- Cardiac Fibrosis and Remodeling
- Hippo pathway signaling and YAP/TAZ
- Cardiomyopathy and Myosin Studies
- Genomics and Chromatin Dynamics
- Cancer, Hypoxia, and Metabolism
- Extracellular vesicles in disease
- Telomeres, Telomerase, and Senescence
- COVID-19 Clinical Research Studies
- PARP inhibition in cancer therapy
- Cancer-related molecular mechanisms research
- Cancer Genomics and Diagnostics
- RNA Research and Splicing
- Coronary Artery Anomalies
- SARS-CoV-2 and COVID-19 Research
- Kruppel-like factors research
- GDF15 and Related Biomarkers
- MicroRNA in disease regulation
- Hormonal Regulation and Hypertension
- Immune Cell Function and Interaction
- Circular RNAs in diseases
- Muscle Physiology and Disorders
Max Planck Institute for Heart and Lung Research
2017-2024
Universities of Giessen and Marburg Lung Center
2024
Justus-Liebig-Universität Gießen
2024
German Center for Lung Research
2024
Cardio-Pulmonary Institute
2024
Goethe University Frankfurt
2022
Abstract Formation and segregation of cell lineages forming the heart have been studied extensively but underlying gene regulatory networks epigenetic changes driving fate transitions during early cardiogenesis are still only partially understood. Here, we comprehensively characterize mouse cardiac progenitor cells (CPCs) marked by Nkx2-5 Isl1 expression from E7.5 to E9.5 using single-cell RNA sequencing transposase-accessible chromatin profiling (ATAC-seq). By leveraging on cell-to-cell...
Pathological cardiac hypertrophy is a leading cause of heart failure, but knowledge the full repertoire cells and their gene expression profiles in human hypertrophic missing. Here, by using large-scale single-nucleus transcriptomics, we present transcriptional response cardiomyocytes to pressure overload caused aortic valve stenosis describe major alterations cellular crosstalk. Hypertrophied had reduced input from endothelial fibroblasts. Genes encoding Eph receptor tyrosine kinases,...
Abstract Individual adult ventricular cardiomyocytes are either mono- or multi-nucleated and undergo morphological changes during cardiac hypertrophy. However, corresponding transcriptional signatures, reflecting potentially different functions the ability for cell-cycle entry, not known. The aim of this study was to determine profile by single-cell RNA-sequencing (scRNA-seq) investigate heterogeneity among under baseline conditions in pressure-induced We developed an array-based approach...
To assess the functional relevance and therapeutic potential of pro-angiogenic long non-coding RNA MANTIS in vascular disease development.RNA sequencing, CRISPR activation, overexpression, RNAi demonstrated that MANTIS, especially its Alu-element, limits endothelial ICAM-1 expression different types cells. Loss increased monocyte adhesion an ICAM-1-dependent manner. reduced binding SWI/SNF chromatin remodelling factor BRG1 at promoter. The was induced by laminar flow HMG-CoA-reductase...
In contrast to adult mammals, zebrafish can fully regenerate injured cardiac tissue, and this regeneration process requires an adequate tightly controlled immune response. However, which components of the response are required during is unclear. Here, we report positive roles for antigen presentation-adaptive immunity axis regeneration. We find that following initial innate response, activated endocardial cells (EdCs), as well cells, start expressing presentation genes. also observe T helper...
Risk stratification of COVID-19 patients is essential for pandemic management. Changes in the cell fitness marker, hFwe-Lose, can precede host immune response to infection, potentially making such a biomarker an earlier triage tool. Here, we evaluate whether hFwe-Lose gene expression outperform conventional methods predicting outcomes (e.g., death and hospitalization) patients. We performed post-mortem examination infected lung tissue deceased determine hFwe-Lose's biological role acute...
SUMMARY Formation and segregation of cell lineages building the vertebrate heart have been studied extensively by genetic tracing techniques analysis single marker gene expression but underlying regulatory networks driving fate transitions during early cardiogenesis are only partially understood. Here, we comprehensively characterized mouse cardiac progenitor cells (CPC) marked Nkx2-5 Isl1 from E7.5 to E9.5 using single-cell RNA sequencing. By leveraging on cell-to-cell heterogeneity,...
Aging is a major risk factor for cardiovascular diseases contributing to the progressive deterioration of heart function. Since vascular niche was shown maintain cardiac homeostasis, we explored epigenomic and transcriptional circuits driving endothelial cell (EC) impairment with aging. Analysis chromatin accessibility by snATAC-seq gene expression RNA-seq hearts from 18- 20-month-old mice revealed significant reduction in zinc finger transcription ZBTB16, which also confirmed aged human...