A5038761050- Cancer-related molecular mechanisms research
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- RNA modifications and cancer
- RNA Research and Splicing
- Viral gastroenteritis research and epidemiology
- RNA and protein synthesis mechanisms
- Plant and Fungal Interactions Research
- Adipokines, Inflammation, and Metabolic Diseases
- vaccines and immunoinformatics approaches
- Circular RNAs in diseases
- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Nuts composition and effects
- Antibiotic Resistance in Bacteria
- Immune responses and vaccinations
- Viral Infections and Vectors
- PARP inhibition in cancer therapy
- interferon and immune responses
- Horticultural and Viticultural Research
- Pneumonia and Respiratory Infections
- Inhalation and Respiratory Drug Delivery
- Viral Infections and Outbreaks Research
- Adipose Tissue and Metabolism
- Animal Virus Infections Studies
West Virginia University
2020-2024
Marshall University
2022
West Virginia Wesleyan College
2018-2019
SARS-CoV-2 is a viral respiratory pathogen responsible for the current global pandemic and disease that causes COVID-19. All WHO approved COVID-19 vaccines are administered through muscular route. We have developed prototype two-dose vaccine (BReC-CoV-2) by combining Receptor Binding Domain (RBD) antigen, via conjugation to Diphtheria toxoid (EcoCRM®). The adjuvanted with Bacterial Enzymatic Combinatorial Chemistry (BECC), BECC470. Intranasal (IN) administration of BreC-CoV-2 in K18-hACE2...
The COVID-19 pandemic has been fueled by SARS-CoV-2 novel variants of concern (VOC) that have increased transmissibility, receptor binding affinity, and other properties enhance disease. goal this study is to characterize unique pathogenesis the Delta VOC strain in K18-hACE2-mouse challenge model. Challenge studies suggested lethal dose was higher than Alpha or Beta strains. To differences strain's pathogenesis, a time-course experiment performed evaluate overall host response variant...
Numerous studies have implicated changes in the Y chromosome male cancers, yet few investigated biological importance of noncoding RNA. Here we identify a group chromosome-expressed long RNA (lncRNA) that are involved non-small cell lung cancer (NSCLC) radiation sensitivity. Radiosensitive NSCLC lines demonstrated dose-dependent induction linc-SPRY3-2/3/4 following irradiation, which was not observed radioresistant lines. Cytogenetics revealed loss (LOY) Gain- and loss-of-function...
SARS-CoV-2 variants of concern (VoCs) are impacting responses to the COVID-19 pandemic. Here we present a comparison USA-WA1/2020 (WA-1) strain with B.1.1.7 and B.1.351 VoCs identify significant differences in viral propagation vitro pathogenicity vivo using K18-hACE2 transgenic mice. Passive immunization plasma from an early pandemic patient resulted outcome VoC-infected WA-1-infected mice were protected by plasma, B.1.1.7-infected partially protected, B.1.351-infected not protected....
SARS-CoV-2 variants of concern (VoC) are impacting responses to the COVID-19 pandemic. Here, we utilized passive immunization using human convalescent plasma (HCP) obtained from a critically ill patient in early pandemic study efficacy polyclonal antibodies generated ancestral against Alpha, Beta, and Delta VoC K18 angiotensin converting enzyme 2 (hACE2) transgenic mouse model. HCP protected mice challenge with original WA-1 strain; however, only partially challenged Alpha (60% survival)...
Severe outcomes from SARS-CoV-2 infection are highly associated with preexisting comorbid conditions like hypertension, diabetes, and obesity. We utilized the diet-induced obesity (DIO) model of metabolic dysfunction in K18-hACE2 transgenic mice to as a COVID-19 comorbidity. Female DIO, but not male DIO challenged were observed have shortened time morbidity compared controls. Increased susceptibility female was increased viral RNA burden interferon production males. Transcriptomic analysis...
The ongoing COVID-19 pandemic has contributed largely to the global vaccine disparity. Development of protein subunit vaccines can help alleviate shortages delivered low-income countries. Here, we evaluated efficacy a three-dose virus-like particle (VLP) composed hepatitis B surface antigen (HBsAg) decorated with receptor binding domain (RBD) from Wuhan or Beta SARS-CoV-2 strain adjuvanted either aluminum hydroxide (alum) squalene in water emulsion (SWE). RBD HBsAg were compared standard two...
Abstract The COVID-19 pandemic has been fueled by novel variants of concern (VOC) that have increased transmissibility, receptor binding affinity, and other properties enhance disease. goal this study is to characterize unique pathogenesis the Delta VOC strain in K18-hACE2-mouse challenge model. Challenge studies suggested lethal dose was higher than Alpha or Beta strains. To differences strain’s pathogenesis, a time-course experiment performed evaluate overall host response variant...
Historically, the Y chromosome has presented challenges to classical methodology and philosophy of understanding differences between males females. A genetic unsolved puzzle, was last be fully sequenced. With advent Human Genome Project came a realization that human genome is more than just genes encoding proteins, an entire universe RNA discovered. This dark matter biology black box surrounding have collided over few years, as increasing numbers non-coding RNAs been identified across length...
Long noncoding RNAs (lncRNAs) are relatively novel with increasingly prominent roles in regulating genetic expression, mainly the nucleus but more recently regions such as mitochondrion. This study explores how lncRNAs interact polynucleotide phosphorylase (PNPase), a protein that regulates RNA import into Machine learning identified several structural features improved lncRNA binding to PNPase, which may be useful targeting therapeutics
Emergence of variants concern (VOC) during the COVID-19 pandemic has contributed to decreased efficacy therapeutic monoclonal antibody treatments for severe cases SARS-CoV-2 infection. In addition, cost creating these is high, making their implementation in low- middle-income countries devastated by very difficult. Here, we explored use polyclonal EpF(ab’) 2 antibodies generated through immunization horses with WA-1 RBD conjugated HBsAg nanoparticles as a low-cost treatment disease. We...
Risk stratification of COVID-19 patients is essential for pandemic management. Changes in the cell fitness marker, hFwe-Lose, can precede host immune response to infection, potentially making such a biomarker an earlier triage tool. Here, we evaluate whether hFwe-Lose gene expression outperform conventional methods predicting outcomes (e.g., death and hospitalization) patients. We performed post-mortem examination infected lung tissue deceased determine hFwe-Lose's biological role acute...
Abstract SARS-CoV-2 infection results in wide-ranging disease manifestation from asymptomatic to potentially lethal. Infection poses an increased threat of severity at-risk populations including those with hypertension, diabetes, and obesity. Type 2 Diabetes (T2DM), is characterized, part, by insulin insensitivity impaired glucose regulation. T2DM patients have poorer outcomes COVID-19. We utilized the diet-induced obesity (DIO) model SARS-CoV-2-susceptible K18-hACE2 transgenic mice better...
<p>Table S1 to S4; Figures S12</p>
<div>Abstract<p>Numerous studies have implicated changes in the Y chromosome male cancers, yet few investigated biological importance of noncoding RNA. Here we identify a group chromosome–expressed long RNA (lncRNA) that are involved non–small cell lung cancer (NSCLC) radiation sensitivity. Radiosensitive NSCLC lines demonstrated dose-dependent induction <i>linc-SPRY3-2/3/4</i> following irradiation, which was not observed radioresistant lines. Cytogenetics revealed...
<div>Abstract<p>Numerous studies have implicated changes in the Y chromosome male cancers, yet few investigated biological importance of noncoding RNA. Here we identify a group chromosome–expressed long RNA (lncRNA) that are involved non–small cell lung cancer (NSCLC) radiation sensitivity. Radiosensitive NSCLC lines demonstrated dose-dependent induction <i>linc-SPRY3-2/3/4</i> following irradiation, which was not observed radioresistant lines. Cytogenetics revealed...
<p>Table S1 to S4; Figures S12</p>
The Keystone Symposium 'Small Regulatory RNAs: From Bench to Bedside' was held in Santa Fe, New Mexico from May 1–4, 2022. symposium organized by Frank J. Slack, Jörg Vogel, Ivan Martinez and Karyn Schmidt, brought together scientists working noncoding RNA biology, therapeutics, technologies address mechanistic questions about small regulatory RNAs facilitate translation of these findings into clinical applications. conference addressed four specific aims: Aim 1. Focus on the exciting...
The composition of soil is determined by plants and their organic waste that has been broken down recycled throughout the ecosystem. This process controlled bugs within soil, called litter fauna. analysis fauna under American Chestnut tree species gives details on effects plant environment. chestnut functionally extinct since introduction Chinese blight to America in 1910’s. used dominate ecosystems drive industry related timber nut harvest. Hybridization different changed morphology over...
Lyme disease is the most wide-spread vector-borne in eastern United States. caused by a bacterium, Borrelia burgdorferi, that transmitted tick vector, commonly Ixodes scapularus. In this study, 400 ticks were collected from around Upshur county, West Virginia, and tested PCR for presence of B. burgdorferi DNA. Tick specimen belong to scapularus, Rhipicephalus sanguineus, or are unidentified. One unidentified sample, which positive has been tentatively identified as new invasive Haemaphysalis...
Abstract Background: The pathogenesis of type 2 diabetes mellitus is known to alter cardiac mitochondrial bioenergetics, but the role non-coding RNAs (ncRNAs) in regulating this process remains poorly understood, specifically within mitochondrion. Here we characterized localization nuclear-encoded lncRNAs heart an effort understand how import can modify function during mellitus. Methods: Human patient and FVB/NJ mouse tissues were collected from diabetics non-diabetics. Immunoblotting was...
Abstract Pseudomonas aeruginosa (Pa) is an opportunistic Gram-negative pathogen that can lead to life-threatening pulmonary infections. Several promising vaccine candidates against Pa evaluated in clinical trials. However, there still no commercial available this pathogen, part due a lack of clarity protective correlates vaccine–induced immunity. To elucidate protection Pa, we used intranasal whole–cell adjuvanted with curdlan WCV murine vaccination and challenge model. Immunization...