A5028288310- Ovarian cancer diagnosis and treatment
- Endometrial and Cervical Cancer Treatments
- CAR-T cell therapy research
- Cancer Research and Treatments
- BRCA gene mutations in cancer
- Cancer Genomics and Diagnostics
- Cancer-related molecular mechanisms research
- Virus-based gene therapy research
- Intraperitoneal and Appendiceal Malignancies
- Immunotherapy and Immune Responses
- Cancer Cells and Metastasis
- Cancer Immunotherapy and Biomarkers
- Radiomics and Machine Learning in Medical Imaging
- Monoclonal and Polyclonal Antibodies Research
- Economic and Financial Impacts of Cancer
- PARP inhibition in cancer therapy
- Statistical Methods and Inference
- Epigenetics and DNA Methylation
- Endometriosis Research and Treatment
- Global Cancer Incidence and Screening
- Cervical Cancer and HPV Research
- Prenatal Screening and Diagnostics
- Immune Cell Function and Interaction
- MicroRNA in disease regulation
- Advanced Proteomics Techniques and Applications
Fred Hutch Cancer Center
2016-2025
Seattle University
1994-2023
Swedish Medical Center
2008-2023
Cancer Research Center
1997-2022
Providence College
2022
St. Joseph Health System
2022
Oncology Specialists
2008-2019
Cape Town HVTN Immunology Laboratory / Hutchinson Centre Research Institute of South Africa
2004-2018
Swedish Medical Center
1998-2015
University of Washington
1997-2015
Improved approaches for the detection of common epithelial malignancies are urgently needed to reduce worldwide morbidity and mortality caused by cancer. MicroRNAs (miRNAs) small (≈22 nt) regulatory RNAs that frequently dysregulated in cancer have shown promise as tissue-based markers classification prognostication. We show here miRNAs present human plasma a remarkably stable form is protected from endogenous RNase activity. originating prostate xenografts enter circulation, readily measured...
Currently, screening for ovarian cancer is not recommended the general population. Targeting women with specific symptoms has been evaluated only recently, because it was believed that had limited specificity.A case-control study of 149 cancer, including 255 who were in a program and 233 referred pelvic/abdominal ultrasound, conducted by inviting to complete survey symptoms. Patients divided randomly into an exploratory group confirmatory group. Symptom types, frequency, severity, duration...
MicroRNAs (miRNAs) are small regulatory RNAs that implicated in cancer pathogenesis and have recently shown promise as blood-based biomarkers for detection. Epithelial ovarian is a deadly disease which improved outcomes could be achieved by successful early detection enhanced understanding of molecular leads to therapies. A critical step toward these goals establish comprehensive view miRNAs expressed epithelial tissues well normal surface cells.We used massively parallel pyrosequencing...
CA125, human epididymis protein 4 (HE4), mesothelin, B7-H4, decoy receptor 3 (DcR3), and spondin-2 have been identified as potential ovarian cancer biomarkers. Except for their behavior in the prediagnostic period has not evaluated.Immunoassays were used to determine concentrations of HE4, DcR3, proteins serum specimens (1-11 samples per participant) that contributed 0-18 years before diagnosis from 34 patients with (15 advanced-stage serous carcinoma) during a comparable time interval...
In our studies of ovarian cancer cells we have identified subpopulations that are in a transitory E/M hybrid stage, i.e. simultaneously express epithelial and mesenchymal markers. not homogenous but, vitro vivo, contain subsets can be distinguished based on number phenotypic features, including the subcellular localization E-cadherin, expression levels Tie2, CD133, CD44. A cellular subset (E/M-MP) (membrane E-cadherinlow/cytoplasmic E-cadherinhigh/CD133high, CD44high, Tie2low) is highly...
Abstract The cellular adaptive immune system mounts a response to many solid tumours mediated by tumour‐infiltrating T lymphocytes ( TILs ). Basic measurements of these , including total count, show promise as prognostic markers for variety cancers, ovarian and colorectal. In addition, recent therapeutic advances are thought exploit this effectively fight melanoma, with promising studies showing efficacy in additional cancers. However, the basic properties poorly understood, specificity,...
Purpose: Women at familial/genetic ovarian cancer risk often undergo screening despite unproven efficacy. Research suggests each woman has her own CA125 baseline; significant increases above this level may identify cancers earlier than standard 6- to 12-monthly > 35 U/mL.Experimental Design: Data from prospective Cancer Genetics Network and Gynecologic Oncology Group trials, which screened 3,692 women (13,080 woman-screening years) with a strong breast/ovarian family history or BRCA1/2...
Abstract Early detection of ovarian cancer remains an important unmet medical need. Effective screening could reduce mortality by 10%–30%. Used individually, neither serum CA125 nor transvaginal sonography (TVS) is sufficiently sensitive or specific. Two-stage strategies have proven more effective, where a significant rise above woman's baseline prompts TVS and abnormal sonogram surgery. Two major trials documented that this strategy has adequate specificity, but sensitivity for early-stage...
To improve the understanding of chemo-refractory high-grade serous ovarian cancers (HGSOCs), we characterized proteogenomic landscape 242 (refractory and sensitive) HGSOCs, representing one discovery two validation cohorts across biospecimen types (formalin-fixed paraffin-embedded frozen). We identified a 64-protein signature that predicts with high specificity subset HGSOCs refractory to initial platinum-based therapy is validated in independent patient cohorts. detected significant...
Abstract Purpose: Efforts to validate ovarian cancer early detection biomarkers with immunoassays are challenged by the limited specimen volumes available. We sought develop a specimen-efficient assay measure CA125 in serum, assess its reproducibility, validity, and performance, test potential for multiplexing combining human epididymis protein 4 (HE4), promising novel marker. Experimental Design: Four pairs of commercially available anti-CA125 antibodies one pair anti-HE4 were evaluated...
Presence of intratumoral T-cell infiltration has been linked to improved survival in ovarian cancer patients. We questioned whether antibody immunity specific for tumor antigens would predict disease outcome. evaluated humoral immune responses against p53, HER-2/neu, and topoisomerase IIalpha.Serum was collected from 104 women (median age, 59 years; range, 34 89 years) at the time their initial definitive surgery cancer. Serum analyzed by enzyme-linked immunosorbent assay antibodies IIalpha...
BackgroundElucidation of the repertoire secreted and cell surface proteins tumor cells is relevant to molecular diagnostics, imaging targeted therapies. We have characterized proteome released into extra-cellular milieu three ovarian cancer lines, CaOV3, OVCAR3 ES2 enriched from ascites fluid.Methodology FindingsTo differentiate media protein constituents utilized for culture, were grown in presence [13C]-labeled lysine. A biotinylation-based approach was used capture associated proteins....
Ovarian cancer is the second most common gynecologic malignancy in US and—because more than 70% of women affected are diagnosed with advanced-stage disease—the deadliest. Contrary to what might be expected, screening has not lessened mortality even high-risk populations. The idea targeting having specific symptoms screened relatively recent because general belief that have limited specificity. authors undertook a case-control study 149 ovarian cancer. An exploratory group served estimate...
Epithelial ovarian carcinoma is a significant cause of cancer mortality in women worldwide and the United States. comprises several histological subtypes, each with distinct clinical molecular characteristics. The natural history this heterogeneous disease, including cell types origin, poorly understood. This study applied recently developed methods for high-throughput DNA methylation profiling to characterize lines tumors, representatives three major histologies.We obtained profiles 1,505...
Abstract Objective: To evaluate the effect of ovarian cancer risk on performance serum biomarkers mesothelin, human epididymis protein 4 (HE4), and CA125. Methods: We measured HE4, CA125 levels from women with invasive (n = 143), benign gynecologic conditions 124), controls 344). Demographic, epidemiologic, reproductive, medical, family history data were collected using a standardized questionnaire. Pedigree BRCA 1/2 test results used to stratify into average high-risk groups. The diagnostic...