A5029015916- HIV Research and Treatment
- HIV-related health complications and treatments
- Cellular transport and secretion
- HIV/AIDS Research and Interventions
- Neuroscience and Neuropharmacology Research
- Plant Molecular Biology Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Photosynthetic Processes and Mechanisms
- RNA Interference and Gene Delivery
- Cholesterol and Lipid Metabolism
- Adenosine and Purinergic Signaling
- Autophagy in Disease and Therapy
- Pancreatic function and diabetes
- Digestive system and related health
- Amino Acid Enzymes and Metabolism
- Lysosomal Storage Disorders Research
- Caveolin-1 and cellular processes
- Nephrotoxicity and Medicinal Plants
- Drug-Induced Hepatotoxicity and Protection
- Lipid Membrane Structure and Behavior
- Retinal Development and Disorders
- Cannabis and Cannabinoid Research
- Plant Reproductive Biology
- Cytomegalovirus and herpesvirus research
- Drug Transport and Resistance Mechanisms
The University of Texas Medical Branch at Galveston
2020-2022
Albert Einstein College of Medicine
2020
Universidad Andrés Bello
2009
Pontificia Universidad Católica de Chile
2003-2008
Universidad de Extremadura
1996
The human immunodeficiency virus (HIV) enters the central nervous system (CNS) within a few days after primary infection, establishing viral reservoirs that persist even with combined antiretroviral therapy (cART). We show monocytes from people living HIV (PLWH) on suppressive cART harboring integrated HIV, mRNA, and/or proteins preferentially transmigrate across blood-brain barrier (BBB) to CCL2 and are significantly enriched post-transmigration, more highly posttransmigration than T cells...
In addition to hemostasis, human platelets have several immune functions and interact with infectious pathogens including HIV in vitro. Here, we report that from HIV-infected individuals on combined antiretroviral drug therapy (ART) low blood CD4+ T cell counts (<350 cells/μl) contained replication-competent despite viral suppression. vitro, harboring propagated the virus macrophages, a process could be prevented biologic abciximab, an anti-integrin αIIb/β3 Fab. Furthermore, our cohort, 88%...
The major barrier to cure HIV infection is the early generation and extended survival of reservoirs in circulation tissues. Currently, techniques used detect quantify are mostly based on blood-based assays; however, it has become evident that viral remain Our study describes a novel multi-component imaging method (HIV DNA, mRNA, proteins same assay) identify, quantify, characterize tissues blood products obtained from HIV-infected individuals even when systemic replication undetectable. In...
In non-neuronal cells, inactivation of protein kinase D (PKD) blocks fission trans -Golgi network (TGN) transport carriers, inducing the appearance long tubules filled with cargo. We now report on function PKD1 in neuronal trafficking. cultured hippocampal pyramidal transferrin receptor (TfR) and low-density receptor-related (LRP) are predominantly transported to dendrites excluded from axons. Expression kinase-inactive or its depletion by RNA interference treatment dramatically selectively...
Megalin and the low‐density lipoprotein (LDL) receptor‐related protein (LRP) are two large members of LDL receptor family that bind endocytose multiple ligands. The molecular cellular determinants dictate sorting behavior these receptors in polarized epithelial cells largely unknown. is found apically distributed, whereas limited information on LRP indicates its polarity. We show here Madin‐Darby canine kidney cells, both endogenous a minireceptor containing fourth ligand‐binding,...
Low-density lipoprotein receptor-related protein 1 (LRP1) is an endocytic recycling receptor with two cytoplasmic tyrosine-based basolateral sorting signals. Here we show that during biosynthetic trafficking LRP1 uses AP1B adaptor complex to move from a post-TGN endosome (RE) the membrane. Then it recycles basolaterally (BSE) involving recognition by nexin 17 (SNX17). In pathway, Y(29) but not N(26) proximal NPXY directs TGN. A N(26)A mutant revealed this motif recognized SNX17 required for...
Uridine 5'-diphosphate (UDP)-glucose is transported into the lumen of endoplasmic reticulum (ER), and Arabidopsis nucleotide sugar transporter AtUTr1 has been proposed to play a role in this process; however, different lines evidence suggest that another transporter(s) may also be involved. Here we show AtUTr3 involved transport UDP-glucose located at ER but Golgi. Insertional mutants showed no obvious phenotype. Biochemical analysis both indicates uptake mostly driven by these two...
Abstract HIV has become a chronic disease despite the effective use of antiretroviral therapy (ART). However, mechanisms tissue colonization, viral evolution, generation reservoirs, and compartmentalization are still matter debate due to challenges involved in examining early events infection at cellular molecular level. Thus, there is an urgent need explore these areas develop cure strategies. In this study, we describe colonization as well role tunneling nanotube–like structures during...
Abstract In healthy conditions, pannexin‐1 (Panx‐1) channels are in a close state, but several pathological including human immunodeficiency virus‐1 (HIV) and NeuroHIV, the channel becomes open. However, mechanism or contribution of Panx‐1 to HIV pathogenesis NeuroHIV is unknown. To determine we used well‐established model simian virus (SIV) infection macaques ( Macaca mulatta ) presence absence blocker later examine synaptic/axonal compromise induced for virus. Using Golgi's staining,...
Abstract Autophagy is an evolutionarily conserved, highly regulated catabolic process critical to neuronal homeostasis, function and survival throughout organismal lifespan. However, the external factors signals that control autophagy in neurons are still poorly understood. Here we report G protein-coupled metabotropic glutamate receptor 1 (mGlu1) contributes basal brain. upregulated brain of adult mGlu1 knockout mice genetic deletion or pharmacological inhibition native receptors enhances...
Background: The lysine salt of bendazac is a non-steroidal anti-inflammatory agent, and it marketed exclusively for the treatment cataracts. We report two cases possible hepatotoxicity due to use lysine. Methods: Laboratory tests, serologic abdominal sonography scan were performed study liver disease. Results: Reversible increases hepatic enzymes found in both cases. Anemia was also one (case 1). Conclusions: Abnormal test results could be related injury attributed