A5039207046- Cellular transport and secretion
- Retinal Development and Disorders
- Cell Adhesion Molecules Research
- Microtubule and mitosis dynamics
- Ubiquitin and proteasome pathways
- Neuroscience and Neuropharmacology Research
- Cellular Mechanics and Interactions
- Genetic Neurodegenerative Diseases
- HIV/AIDS drug development and treatment
- Calcium signaling and nucleotide metabolism
- ATP Synthase and ATPases Research
- Glycosylation and Glycoproteins Research
- Endoplasmic Reticulum Stress and Disease
- Galectins and Cancer Biology
- Axon Guidance and Neuronal Signaling
- Erythrocyte Function and Pathophysiology
- Photoreceptor and optogenetics research
- Lipid Membrane Structure and Behavior
- Muscle Physiology and Disorders
- Mitochondrial Function and Pathology
- Genetic Associations and Epidemiology
- TGF-β signaling in diseases
- Nerve injury and regeneration
- Biochemical and Molecular Research
- Protein Tyrosine Phosphatases
Instituto de Investigación Médica Mercedes y Martín Ferreyra
2009-2022
Consejo Nacional de Investigaciones Científicas y Técnicas
2010-2022
Universidad Nacional de Córdoba
1994-2021
Marqués de Valdecilla University Hospital
2018
Institute for Medical Research
2007
Research Centre in Biological Chemistry of Córdoba
1997-2001
CIUDAD
1997
Hospital de São João
1997
In non-neuronal cells, inactivation of protein kinase D (PKD) blocks fission trans -Golgi network (TGN) transport carriers, inducing the appearance long tubules filled with cargo. We now report on function PKD1 in neuronal trafficking. cultured hippocampal pyramidal transferrin receptor (TfR) and low-density receptor-related (LRP) are predominantly transported to dendrites excluded from axons. Expression kinase-inactive or its depletion by RNA interference treatment dramatically selectively...
c-Fos, a transcription factor that constitutes DNA-binding AP-1 complexes, regulates gene expression promotes long-lasting cellular changes. We show that, in addition to its activity, c-Fos the metabolism of phospholipids cytoplasmically by an AP-1-independent activity. Two waves promote subsequent stimulation 32P-orthophosphate incorporation into are evidenced quiescent cultured fibroblasts induced re-enter cell cycle. The first wave peaks at 7.5 min and returns control levels 15 min....
RhoA and Rac play key opposite roles during neuronal polarization. We now show that Lfc, a guanosine nucleotide exchange factor (GEF), localizes to the Golgi apparatus growth cones of developing neurons negatively regulates neurite sprouting axon formation through Rho signaling pathway. Tctex-1, dynein light chain implicated in outgrowth by modulating actin dynamics activity, colocalizes physically interacts with thus inhibiting its GEF decreasing Rho-GTP levels, functionally antagonizing...
PTP1B is an endoplasmic reticulum (ER) anchored enzyme whose access to substrates partly dependent on the ER distribution and dynamics. One of these substrates, protein tyrosine kinase Src, has been found in cytosol, endosomes, plasma membrane. Here we analyzed where Src physically interact intact cells, by bimolecular fluorescence complementation (BiFC) combination with temporal high resolution microscopy. We also determined structural basis this interaction. that BiFC signal displayed as...
Background. The insertion/deletion (I/D) angiotensin-converting enzyme (ACE) polymorphism has been established as a cardiovascular risk factor in some populations, but the association with essential hypertension is controversial. Predominantly diastolic (PDH), or narrow pulse pressure hypertension, shown to have increased peripheral resistance. Because DD genotype associated higher plasma ACE levels and angiotensin II activity, we genotyped PDH patients for I/D polymorphism. Methods....
SARA (Smad Anchor for Receptor Activation) plays a crucial role in Rab5-mediated endocytosis cell lines localizing to early endosomes where it regulates morphology and function. Here, we analyzed the of during neuronal development tested whether functions as regulator endocytic trafficking selected axonal membrane proteins. Suppression perturbs appearance juxtanuclear recycling compartments neurons show long axons with large growth cones. Furthermore, surface distribution adhesion molecule...
Emerging evidence suggests that endocytic trafficking of adhesion proteins plays a critical role in neuronal migration during neocortical development. However, the molecular insights these processes remain elusive. Here we study an early endosomal protein Smad Anchor for Receptor Activation (SARA) developing mouse brain. SARA is enriched at apical endfeet radial glia neocortex. While silencing did not lead to detectable neurogenic phenotypes, SARA-suppressed neurons exhibit impaired...
The acquisition of neuronal polarity is a complex molecular process that depends on changes in cytoskeletal dynamics and directed membrane traffic, regulated by the Rho Rab families small GTPases, respectively. However, during axon specification, link couples these protein has yet to be identified. In this paper, we describe new positive feedback loop between Rab8a Cdc42, coupled Tuba, Cdc42-specific guanine nucleotide-exchange factor (GEF), ensures single generation rodent hippocampal...
Abstract Slow saccades, postural/intention tremor, peripheral neuropathy, and decreased deep‐tendon reflexes are valuable neurological signs for clinical suspicion of spinocerebellar ataxia type 2 (SCA2). We report the presence abnormally brisk in nonsymptomatic carriers mildly severely affected subjects a large Argentinean SCA2 pedigree. The identification this distinctive phenotype an entire pedigree reinforces current concept that algorithms limited value as indicators genetic testing...