A5025092128- Lipid Membrane Structure and Behavior
- Glycosylation and Glycoproteins Research
- Protein Interaction Studies and Fluorescence Analysis
- Protein Structure and Dynamics
- Antimicrobial Peptides and Activities
- Surfactants and Colloidal Systems
- Venomous Animal Envenomation and Studies
- Alzheimer's disease research and treatments
- Supramolecular Self-Assembly in Materials
- Healthcare and Venom Research
- Cellular transport and secretion
- Proteins in Food Systems
- Sphingolipid Metabolism and Signaling
- Monoclonal and Polyclonal Antibodies Research
- Spectroscopy and Quantum Chemical Studies
- thermodynamics and calorimetric analyses
- Biochemical and Structural Characterization
- Enzyme Structure and Function
- Protein Kinase Regulation and GTPase Signaling
- Biotin and Related Studies
- Protein purification and stability
- Molecular Junctions and Nanostructures
- Ion channel regulation and function
- RNA and protein synthesis mechanisms
- Lipid metabolism and biosynthesis
Research Centre in Biological Chemistry of Córdoba
2016-2025
Universidad Nacional de Córdoba
2016-2025
Consejo Nacional de Investigaciones Científicas y Técnicas
2015-2025
National University of Rosario
2001
University of the Basque Country
1996-2000
University of Illinois Urbana-Champaign
1999
Fluorescence Innovations (United States)
1999
UC Irvine Health
1999
CIUDAD
1997
Rede de Química e Tecnologia
1995
Parkinson's disease is an age-related movement disorder characterized by the presence in mid-brain of amyloid deposits 140-amino-acid protein AS (α-synuclein). fibrillation follows a nucleation polymerization pathway involving diverse transient prefibrillar species varying size and morphology. Similar to other neurodegenerative diseases, cytotoxicity currently attributed these rather than insoluble aggregates. Nevertheless, underlying molecular mechanisms responsible for remain elusive...
The effect of biotin binding on streptavidin (STV) structure and stability was studied using differential scanning calorimetry, Fourier transform infrared spectroscopy (FT-IR), fluorescence spectroscopy. Biotin increases the midpoint temperature Tm, thermally induced denaturation STV from 75 degrees C in unliganded protein to 112 at full ligand saturation. cooperativity unfolding changes substantially presence biotin. Unliganded monomer has least one domain that unfolds independently. dimer...
The interaction between ligands and proteins usually induces changes in protein thermal stability with modifications the midpoint denaturation temperature, enthalpy of unfolding, heat capacity. These are due to coupling unfolding binding equilibrium. Furthermore, they can be attained by structure conformational flexibility induced ligand interaction. To study these effects we have used bovine serum albumin (BSA) interacting three different anilinonaphthalene sulfonate derivatives (ANS). on...
Abstract Although 6‐lauroyl‐2‐( N , ‐dimethylamino)naphthalene (LAURDAN) is now widely used as a probe for lipid systems, most studies focus on the effect of environment its emission properties but not excitation properties. The present study intended to investigate LAURDAN in diverse environments. To this end, fluorescence were studied synthetic ester and ether phosphatidylcholines sphingomyelin vesicles below, at above corresponding main phase‐transition temperature. spectra these...
A model for the interaction of 6-lauroyl-2-(N,N-dimethylamino)
The catalytic behaviour of alpha-CT (alpha-chymotrypsin) is affected by cationic micelles CTABr (hexadecyltrimethylammonium bromide). enzyme-micelle interaction leads to an increase in both the V(max) and affinity for substrate p -nitrophenyl acetate, indicating higher efficiency bound alpha-CT. bell-shaped profile activity with increasing concentrations suggests that micelle-bound enzyme reacts free substrate. Although more active micelles, stability essentially same as observed buffer...
1. The interactions of four proteins (albumin, myelin basic protein, melittin and glycophorin) with eight neutral or acidic glycosphingolipids, including sulphatides gangliosides, five zwitterionic anionic phospholipids some their mixtures, were studied in lipid monolayers at the air/145 mM-NaCl interface. 2. In lipid-free interfaces, surface pressure potential reached by either soluble integral membrane did not reveal marked differences. 3. All showed each lipids but maximal found for...
The effect of neutral (galactocerebroside and asialo-ganglioside GM1) or anionic (sulphatide gangliosides GM1, GD1a GT1b) glycosphingolipids on the activity phospholipase A2 from pig pancreas was studied in mixed monolayers dilauroyl phosphatidylcholine with different molar fractions at various constant surface pressures. enzyme depends proportion type glycosphingolipid interface. Sulphatide activates all proportions, whereas galactocerebroside shows inhibition activation depending its film....
In this work; we investigated the differential interaction of amphiphilic antimicrobial peptides with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) lipid structures by means extensive molecular dynamics simulations. By using a coarse-grained (CG) model within MARTINI force field; simulated peptide-lipid system from three different initial configurations: (a) in water presence pre-equilibrated bilayer; (b) inside hydrophobic core membrane; and (c) random configurations that allow...