A5083861715- Lipid Membrane Structure and Behavior
- Antimicrobial Peptides and Activities
- Cellular transport and secretion
- HIV Research and Treatment
- RNA Interference and Gene Delivery
- Layered Double Hydroxides Synthesis and Applications
- Alzheimer's disease research and treatments
- Supramolecular Self-Assembly in Materials
- Biochemical and Structural Characterization
- Erythrocyte Function and Pathophysiology
- Sphingolipid Metabolism and Signaling
- Spectroscopy and Quantum Chemical Studies
- Photoreceptor and optogenetics research
- Protein Structure and Dynamics
- Polydiacetylene-based materials and applications
- Mesoporous Materials and Catalysis
- Polymer Surface Interaction Studies
- Surfactants and Colloidal Systems
- Glycosylation and Glycoproteins Research
- Mosquito-borne diseases and control
- RNA Research and Splicing
- Healthcare and Venom Research
- Pickering emulsions and particle stabilization
- Biofield Effects and Biophysics
- Protein purification and stability
Research Centre in Biological Chemistry of Córdoba
2015-2024
Consejo Nacional de Investigaciones Científicas y Técnicas
2007-2024
Universidad Nacional de Córdoba
2015-2024
University of Liège
2023
Centre National de la Recherche Scientifique
2008-2009
Institut Curie
2008-2009
Biologie cellulaire et Cancer
2008
Cytoplasmic coat proteins are required for cargo selection and budding of tubulovesicular transport intermediates that shuttle between intracellular compartments. To better understand the physical parameters governing assembly coat-induced membrane deformation, we have reconstituted Arf1-dependent COPI on giant unilamellar vesicles by using fluorescently labeled Arf1 coatomer. Membrane recruitment Arf1-GTP occurs exclusively disordered lipid domains does not induce optically visible...
Dengue virus (DENV) and Zika (ZIKV) capsid proteins efficiently recruit surround the viral RNA at endoplasmic reticulum (ER) membrane to yield nascent particles. However, little is known either about molecular mechanisms by which multiple copies of assemble into nucleocapsids (NCs) or how NC recruited wrapped ER during particle morphogenesis. Here, we measured relevant interactions concerning this process using purified DENV ZIKV proteins, membranes mimicking lipid composition, nucleic acids...
The impact of lipid diversity on the lateral organization biological membranes remains a topic debate. While existence domains in lamellar is well-established, nonlamellar phases occurring systems are less explored due to technical constraints. Here, we present measurement excitation–emission matrices (EEM) LAURDAN several structures. fluorescence probe widely used for characterizing assemblies. EEMs were analyzed by multidimensional spectral phasors (MdSP), an approach that seizes...
In this work; we investigated the differential interaction of amphiphilic antimicrobial peptides with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) lipid structures by means extensive molecular dynamics simulations. By using a coarse-grained (CG) model within MARTINI force field; simulated peptide-lipid system from three different initial configurations: (a) in water presence pre-equilibrated bilayer; (b) inside hydrophobic core membrane; and (c) random configurations that allow...
Arfaptin2 contains a Bin/Amphiphysin/Rvs (BAR) domain and directly interacts with proteins of the Arf/Arl family in their active GTP-bound state. It has been proposed that BAR domains are able to sense membrane curvature induce tubulation. We report here Arf1 is required for recruitment artificial liposomes mimicking Golgi apparatus lipid composition. The Arf1-dependent increases curvature, while itself not sensitive curvature. At high protein concentrations, binding induces Finally,...
The amyloid aggregation of the presynaptic protein α-synuclein (AS) is pathognomonic Parkinson's disease and other neurodegenerative disorders. Physiologically, AS contributes to synaptic homeostasis by participating in vesicle maintenance, trafficking, release. Its avidity for highly curved acidic membranes has been related distinct chemistry N-terminal amphipathic helix adopted upon binding appropriated lipid interfaces. Pathologically, populate a myriad toxic aggregates ranging from...
Abstract Lipid packing defects favor the binding of proteins to cellular membranes by creating spaces between lipid head groups that allow insertion amphipathic helices or modifications. The density in a membrane is well known increase with curvature and presence conical-shaped lipids. In contrast, role tension formation has been poorly investigated. Here we use combination numerical simulations experiments measure effect on defects. We first monitor ALPS (amphipathic sensor) giant...
Membrane fusion is considered relevant in countless scientific areas and biotechnological processes, ranging from vital life events to biomedicine, pharmaceuticals, materials engineering, among others. In this study, we employed hydrophobic oleic acid (OA)-coated magnetite (Fe3O4) nanoparticles (MNP-OA) as a platform induce the of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphatidylcholine liposomes [large unilamellar vesicles (LUVs)] colloidal dispersion. This was monitored through dynamic light...
The use of phasors to analyze fluorescence data was first introduced for time-resolved studies a simpler mathematical analysis the fluorescence-decay curves. Recently, this approach extended steady-state experiments with introduction spectral (SP), derived from Fourier transform emission spectrum. In work, we revise key aspects that lead an interpretation SP as characteristic function probability distribution. This formalism allows us introduce new tool, called multi-dimensional phasor...