A5078538600- Bacteriophages and microbial interactions
- Microtubule and mitosis dynamics
- Vibrio bacteria research studies
- Plant Virus Research Studies
- SARS-CoV-2 and COVID-19 Research
- Poxvirus research and outbreaks
- Infections and bacterial resistance
- Cellular transport and secretion
- Plant Pathogenic Bacteria Studies
- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- Retinal Development and Disorders
- Microbial bioremediation and biosurfactants
- SARS-CoV-2 detection and testing
- Advanced biosensing and bioanalysis techniques
- Bacterial biofilms and quorum sensing
- CRISPR and Genetic Engineering
- Animal Virus Infections Studies
- Reproductive Biology and Fertility
- Ubiquitin and proteasome pathways
- Plant and Fungal Interactions Research
- Influenza Virus Research Studies
- Hedgehog Signaling Pathway Studies
- Genetic and Kidney Cyst Diseases
- Electrowetting and Microfluidic Technologies
National Yang Ming Chiao Tung University
2024-2025
University of Maryland, Baltimore
2020-2023
Columbus Center
2020-2022
Chia-Yi Christian Hospital
2020-2021
National Chung Hsing University
2012-2021
National Taiwan University
2020
University of Maryland Center for Environmental Science
2020
Taiwan Semiconductor Manufacturing Company (Taiwan)
2020
Johns Hopkins University
2012-2019
National Tsing Hua University
2017
Dynactin is an essential part of the cytoplasmic dynein motor that enhances processivity and serves as adaptor allows to bind cargoes. Much known about dynactin's interaction with microtubules, but how it associates its diverse complement subcellular binding partners remains mysterious. It has been suggested cargo specification involves a group subunits referred "pointed-end complex." We used chemical cross-linking, RNA interference, protein overexpression characterize interactions within...
Aberrant hedgehog (Hh) signaling contributes to the pathogenesis of multiple cancers. Available inhibitors target Smoothened (Smo), which can acquire mutations causing drug resistance. Thus, compounds that inhibit Hh downstream Smo are urgently needed. We identified dynarrestin, a novel inhibitor cytoplasmic dyneins 1 and 2. Dynarrestin acts reversibly dynein 1-dependent microtubule binding motility in vitro without affecting ATP hydrolysis. It rapidly inhibits endosome movement living cells...
Introduction The rpoE-chrR pair is a regulatory system used by photosynthetic microorganisms to overcome singlet oxygen stress. rpoE and chrR encode the sigma factor σ E anti-sigma ChrR, respectively. Stenotrophomonas maltophilia, an opportunistic pathogen, multidrug-resistant gram-negative bacterium. Although it not microorganism, homolog ( smlt2377-smlt2378 ) was found in S. maltophilia genome. In this study, we aimed assess significance of Ec -ChrR oxidative stress alleviation antibiotic...
Stenotrophomonas maltophilia, an opportunistic pathogen, is ubiquitously distributed in the environment. In response to iron-depletion stress, S. maltophilia synthesizes sole catecholate-type siderophore, stenobactin, for ferric iron acquisition. FepAsm, a TonB-dependent transporter (TBDT), known outer membrane receptor responsible ferri-stenobactin uptake K279a. However, KJ and its isogenic fepA mutant displayed comparable ability utilize FeCl3 as source growth iron-depleted conditions,...
Outer membrane protein OmpA is composed of two domains, an N-terminal β-barrel structure embedded in the outer and a C-terminal globular domain noncovalently associated with peptidoglycan layer periplasm. Stenotrophomonas maltophilia KJ clinical isolate. In our recent study, we disclosed that KJ∆OmpA299 - 356, deletion mutant, compromised menadione tolerance. Furthermore, involvement σE, σN, ompO ∆ompA299 356-mediated phenotype was proposed. hypothesized there unidentified σN-regulated...
Aim The policy enforcing visiting restriction during the COVID‐19 pandemic may cause feelings of social isolation among residents in long‐term care facilities. This study aimed to explore family members' concerns for their relatives lockdown period, assess level acceptance and determine associated factors. Methods From 156 members interviewed, demographic data, satisfaction with overall quality, worry relatives, arrangement if cluster infections occur facility were recorded. Results Among 83...
COVID-19 vaccination resistance has become a major challenge to prevent global SARS-CoV-2 transmission. Here we report that the coverage rate is inversely correlated mutation frequency of full genome (R2=0.878) and spike gene (R2=0.829) delta variants in 16 countries, suggesting against COVID-19, with other mitigation strategies, critical suppress emergent mutations. Neutrality analysis DH Zeng E tests suggested directional selection was driving force variant evolution. To eliminate...
Post‐Golgi to apical surface delivery in polarized epithelial cells requires the cytoplasmic dynein motor complex. However, nature of dynein–cargo interactions and their underlying regulation are largely unknown. Previous studies have shown that targeting rhodopsin light chain, Tctex‐1, which binds directly both intermediate chain (IC) rhodopsin. In this report, we show S82E mutant mimics Tctex‐1 phosphorylated at serine 82, has a reduced affinity for IC but not Velocity sedimentation...
Satellite RNAs associated with Bamboo mosaic virus (satBaMVs) depend on BaMV for replication and encapsidation. Certain satBaMVs isolated from natural fields significantly interfere replication. The 5′ apical hairpin stem loop (AHSL) of satBaMV is the major determinant in interference In this study, by vivo competition assay, we revealed that sequence structure AHSL, along specific nucleotides (C 60 C 83 ) required replication, are also involved among variants. Moreover, all ends isolates...
To examine transmission and evolution of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in shipboard quarantine the Diamond Princess cruise ship.
We demonstrate two heterogeneous solutions to improve power delivery High-Performance Computing (HPC) processors. The scalable HPC vehicle integrates 7nm CMOS processor chiplets, each with four ARM¯ Cortex¯-A72 cores, that are mounted on a Chip-on-Wafer-on-Substrate (CoWoS¯) silicon interposer [1]. In the first solution, Integrated Passive Device (IPD) capacitors placed directly beneath provide more accessible and effective supply noise decoupling. result is 3.9% higher maximum clock...
ABSTRACT Many integrative filamentous phages not only lack Ff coliphage homologues essential for assembly but also have distinct packaging signals (PS). Their encapsidation remains completely uncharacterized to date. Here we report the first evidence of a PS-dependent checkpoint phage assembly. Suppressor screening PS-deficient identified an unknown protein, PSB15 ( PS - b inding 15 kDa), crucial encapsidation. The WAGFXF motif N-terminus directly binds DNA with conformational change, while...
Cytoplasmic dynein is a motor protein complex involved in microtubule-based cargo movement. Previous biochemical evidence suggests that light chain subunits also exist outside the complex. Here we show rp3 present both cytoplasm and nucleus. Nuclear binds to assembles with transcription factor SATB1 at nuclear matrix-associated structures. Dynein intermediate was detected nucleus, but it dispensable for rp3-SATB1 interaction. facilitates localization of rp3, whereas activity are not...
Introduction The hemin acquisition system is composed of an outer membrane TonB-dependent transporter that internalizes into the periplasm, periplasmic hemin-binding proteins to shuttle hemin, inner transports cytoplasm, and cytoplasmic heme oxygenase release iron. Fur HemP are two known regulators involved in regulation acquisition. Stenotrophomonas maltophilia poorly understood, with exception HemA as a for uptake. Methods Putative candidates responsible were selected via homolog search...
Abstract Seasonal flu and pandemics, which account for millions of infections hundreds thousands deaths, require rapid reliable detection mechanisms to implement preventive therapeutic measures. Current methods viral have limitations in speed, accuracy, accessibility, usability. This project presents a novel, widely applicable diagnostic test that uses modified version rolling circle amplification (RCA) be sensitive, specific, direct RNA targeted, colorimetric operable at room temperature....
ABSTRACT Unlike Ff, the packaging signal (PS) and mechanism of integrative filamentous phage assembly remain largely unknown. Here we revived two Inoviridae prophage sequences, ϕLf2 ϕLf-UK, as infectious virions lysogenize black rot pathogen Xanthomonas campestris pv. . ϕLf-UK genomes consist 6,363 6,062 nucleotides share 85.8% 98.7% identity with ϕLf, respectively. To explore their assembly, first identified 20-26– nucleotide long PS sequences 10 phages. These a DNA hairpin consensus...