Abstract Kaempferol is a natural flavonoid. Previous studies have reported that kaempferol has anti‐proliferation activities and induces apoptosis in many cancer cell lines. However, there are no reports on human osteosarcoma. In this study, we investigate the anti‐cancer effects molecular mechanisms of osteosarcoma cells. Our results demonstrate significantly reduces viabilities U‐2 OS, HOB 143B cells, especially OS cells dose‐dependent manner, but exerts low cytotoxicity fetal osteoblast...

Numerous studies have shown that rutin has anticancer effects. We previously reported induced cell cycle arrest and apoptosis in murine leukemia WEHI-3 cells vitro vivo. However, there are no data showing inhibits human HL-60 vivo a xenograft animal model. Human were implanted into mice treated with vehicle (1% DMSO), (120 mg/kg of body weight) or vinblastine μg/kg weight). Compounds agents injected once every four days intraperitoneally (i.p.) for 36 days. Treatment 120 resulted reduction...

Quercetin is one of the natural components from plant and it induces cell apoptosis in many human cancer lines. However, no available reports show that quercetin altered associated gene expressions gastric cells, thus, we investigated effect on apoptotic death expression AGS cells. Results indicated induced morphological changes reduced total viability via Furthermore, results flow cytometric assay increased reactive oxygen species (ROS) production, decreased levels mitochondrial membrane...

Abstract Antimicrobial peptides, such as bacteriocin, produced by probiotics have become a promising novel class of therapeutic agents for treating infectious diseases. Selected lactic acid bacteria (LAB) isolated from fermented foods with probiotic potential were evaluated various tests, including exopolysaccharide production, antibiotic susceptibility, and bile tolerance, antibacterial activity, cell adhesion cytotoxicity to gastric lines. Six selected LAB strains maintained their high...

Abstract Benzyl isothiocyanate (BITC) and phenethyl (PEITC), a member of the family, have been shown to exhibit antineoplastic ability against many human cancer cells. In this study, we found that exposure osteogenic sarcoma U‐2 OS cells BITC PEITC led induce morphological changes decrease percentage viable in time‐ dose‐dependent manner. induced cell cycle arrest at G2/M phase 48 h treatment inhibited levels regulatory proteins such as cyclin A B1 but promoted level Chk1 p53 arrest. marked...

Prostate cancer has its highest incidence and is becoming a major concern. Many studies have shown that traditional Chinese medicine exhibited antitumor responses. Quercetin, natural polyphenolic compound, been to induce apoptosis in many human cell lines. Although numerous evidences show multiple possible signaling pathways of quercetin apoptosis, there no report address the role endoplasmic reticulum (ER) stress quercetin‐induced PC‐3 cells. The purpose this study was investigate effects...

Chrysophanol (1,8‐dihydroxy‐3‐methylanthraquinone) is one of the anthraquinone compounds, and it has been shown to induce cell death in different types cancer cells. The effects chrysophanol on human lung have not well studied. purpose this study examine chrysophanol‐induced cytotoxic also investigate such influences that involved apoptosis or necrosis A549 cells vitro . Our results indicated decreased viable a dose‐ time‐dependent manner. promoted release reactive oxygen species (ROS) Ca 2+...

Stenotrophomonas maltophilia, an opportunistic pathogen, is ubiquitously distributed in the environment. In response to iron-depletion stress, S. maltophilia synthesizes sole catecholate-type siderophore, stenobactin, for ferric iron acquisition. FepAsm, a TonB-dependent transporter (TBDT), known outer membrane receptor responsible ferri-stenobactin uptake K279a. However, KJ and its isogenic fepA mutant displayed comparable ability utilize FeCl3 as source growth iron-depleted conditions,...

Abstract Casticin, a polymethoxyflavone, is one of the major active components obtained from Fructus viticis , which have been shown to anticancer activities including induce cell apoptosis in human cancer cells. The aim this study was investigate molecular mechanisms by casticin inhibits migration and invasion mouse melanoma B16F10 Cell viability examined MTT assay results indicated that decreased total percentages viable cells dose‐dependent manners. Casticin affected were wound healing...

ABSTRACT Sulforaphane (SFN), one of the isothiocyanates, is a biologically active compound extracted from cruciferous vegetables, and has been shown to induce cytotoxic effects on many human cancer cells including leukemia cells. However, exact molecular mechanism altered gene expression associated with apoptosis unclear. In this study, we investigated SFN‐induced whether or not they went through cell‐cycle arrest induction further examined in HL‐60 Cell viability, distribution, sub‐G1...

Abstract Ouabain, a cardiotonic steroid and specific Na + /K ‐ATPase inhibitor, has potential to induce cancer cell apoptosis but the mechanisms of induced by ouabain are not fully understand. The aim this study was investigate cytotoxic effects on human prostate DU 145 cells in vitro. Cell morphological changes were examined phase contrast microscopy. viability, cycle distribution, apoptosis, DNA damage, production ROS Ca 2+ , mitochondrial membrane (ΔΨ m ) measured flow cytometry assay....

Abstract Curcumin can decrease viable cells through the induction of apoptosis in human lung cancer NCI‐H460 vitro . However, there are no reports that curcumin inhibit vivo In this study, tumour were implanted directly into nude mice and divided randomly four groups to be treated with vehicle, (30 mg/kg body weight), (45 weight) doxorubicin (8 weight). Each agent was injected once every 4 days intraperitoneally (i.p.), treatment starting weeks after inoculation cells. Treatment 30 45 or 8...

Dietary fat has been suggested to be the cause of various health issues. Obesity, hypertension, cardiovascular disease, diabetes, dyslipidemia, and kidney disease are known associated with a high-fat diet (HFD). Obesity conditions, such as type 2 diabetes mellitus nonalcoholic fatty liver (NAFLD), currently worldwide problem. Few prospective pharmaceutical therapies that directly target NAFLD available at present. A Traditional Chinese Medicine, ginseng-plus-Bai-Hu-Tang (GBHT), is widely...

Sulforaphane (SFN) is an isothiocyanate, inducing cytotoxic effects in various human cancer cells, including leukemia cells through cell cycle arrest and apoptosis. However, the effect of SFN on immune responses a mouse model remains to be investigated. The present study investigated whether has WEHI‑3‑induced vivo. Normal BALB/c mice were injected with WEHI‑3 generate model, subsequently treated placebo or (0, 285, 570 1,140 mg/kg) for 3 weeks. Following treatment, all weighted blood...

Cantharidin (CTD) induces cytotoxic effects in different types of human cancer cell; however, to date, there have been no studies on the CTD gene expression lung cells and potential associated signaling pathways. Therefore, present study aimed investigate how affects key genes functional pathways H460 using complementary DNA microarray analysis. Human were cultured for 24 h presence or absence 10 µM CTD; was then examined The results indicated that 8 upregulated > 4‑fold, 29 >3‑4‑fold 156...

Although reports have shown that α-phellandrene (α-PA) is one of the monoterpenes and often used in food perfume industry, our previous studies indicated α-PA promoted immune responses normal mice vivo. However, there no available information to show induced cell apoptosis cancer cells, thus, we investigated effects on morphology, viability, cycle distribution, leukemia WEHI-3 cells vitro. Results morphological changes decreased G0/G1 arrest sub-G1 phase (apoptosis) cells. increased...

Abstract Human glioblastoma (GBM) is one of the common cancer death in adults worldwide, and its metastasis will lead to difficult treatment. Finding compounds for future develop treatment urgent. Bisdemethoxycurcumin (BDMC), a natural product, was isolated from rhizome turmeric ( Curcuma longa ), which has been shown against many human cells. In present study, we evaluated antimetastasis activity BDMC GBM Cell proliferation, cell viability, cellular uptake, wound healing, migration...

ABSTRACT Diallyl trisulfide (DATS), a chemopreventive dietary constituent and extracted from garlic, has been shown to against cultured many types of human cancer cell liens but the fate apoptosis in murine leukemia cells vitro immune responses leukemic mice remain elusive. Herein, we clarified actions DATS on growth inhibition WEHI‐3 used generate vivo , following investigate effects animal model. In study, induced through G0/G1 phase arrest induction caspase‐3 activation. study decreased...

The aim of the present study was to investigate effect chitosan (a naturally derived polymer) on immune responses and glutamic oxaloacetic transaminase (GOT), pyruvic (GPT) lactate dehydrogenase (LDH) levels in WEHI‑3 cell‑generated leukemia mice. Mice were divided into control, acetic acid (vehicle)‑treated, 5 20 mg/kg chitosan‑treated groups. subsequently weighed, blood collected, liver spleen samples isolated weighed. Blood measured for cell markers, underwent phagocytosis natural killer...

Abstract Ouabain, the specific Na + /K ‐ATPase blocker, has biological activity including anti‐proliferative and anti‐metastasis effects in cancer cell. There is no study to show ouabain inhibiting cell migration invasion human osteosarcoma U‐2 OS cells. Thus, we investigated effect of on Results indicated that significantly decreased percentage viable cells at 2.5‐5.0 μM, thus, selected 0.25‐1.0 μM for studies. Ouabain inhibited migration, enzymatic activities MMP‐2, also affected...

Hibiscus sabdariffa is commonly used in daily life and its extract applied widely food cosmetics. However, it has not been evaluated for anti-aging effects.Hibiscus calyx aqueous (HSCAE) shown potential collagenase activity suppression effects, together with tyrosinase inhibition, anti-oxidation as a free radical scavenger. The current investigation demonstrated that HSCAE was cytotoxic skin fibroblasts, significantly decreased ultraviolet B (UVB)-induced reactive oxygen species (ROS) on...

Manganese-dependent superoxide dismutase (MnSOD, SodA) and iron-dependent SOD (FeSOD, SodB) are critical cytosolic enzymes for alleviating stress. Distinct from the singular sodA gene in most bacteria, Stenotrophomonas maltophilia harbors two genes, sodA1 sodA2. The roles of SodA1, SodA2, SodB S. stress were investigated. expression sod genes was determined by promoter–xylE transcriptional fusion assay qRT–PCR. SodA2 sodB expressions proportional to bacterial logarithmic growth, but...