The lack of a suitable in vitro hepatitis B virus (HBV) infectivity model has limited examination the early stages virus-cell interaction. In this study, we used an immortalized cell line derived from human primary hepatocytes, HuS-E/2, to study mechanism HBV infection. infection efficiency was markedly increased after dimethyl sulfoxide (DMSO)-induced differentiation cells. Transmission electron microscopy demonstrated presence intact particles DMSO-treated HBV-infected HuS-E/2 cells, which...

Abstract Rotenone is an inhibitor of the mitochondrial electron transport chain complex I, resulting in generation reactive oxygen species (ROS). has been shown to display anticancer activity through induction apoptosis various cancer cells. However, underlying mechanism still not fully understood. Here, rotenone showed a strong growth inhibitory effect against human breast MCF‐7 DNA flow cytometric analysis, chromatin condensation, and poly (ADP‐ribose) polymerase (PARP) cleavage indicated...

Solanum nigrum Linn (SN) belongs to the Solanaceae family, is a plant growing widely in south Asia, and has been used traditional folk medicine. It believed have antipyretic, diuretic, anticancer, hepatoprotective effects. During summertime, this heavily supplement beverages quench thirst on hot days Taiwan several southern Asian countries. In study, polyphenols anthocyanidin various parts of SN were analyzed by HPLC. The leaves found be richer than stem fruit. contained highest...

Although green tea extract has been reported to suppress hyperlipidemia, it is unclear how extracts prepared from green, oolong, black and pu-erh teas modulate fatty acid synthase expression in rats fed on a high-fructose diet. In this animal study, we evaluated the hypolipidemic hypoleptinemia effect of these four different leaves male Wistar for 12 weeks. The results showed that fructose-rich diet significantly elevated serum triacylglycerols, cholesterol, insulin, leptin concentrations,...

The composition of Morinda citrifolia (M. citrifolia) was determined using high-performance liquid chromatography (HPLC), and the anticancer effects M. extract evaluated in HepG2, Huh7, MDA-MB-231 cancer cells. fruit extracts were obtained by five different organic solvents, including hexane (Hex), methanol (MeOH), ethyl acetate (EtOAc), chloroform (CHCl3), ethanol (EtOH). water-EtOAc from fruits found to have highest activity. HPLC data revealed predominance chrysin fruit. Furthermore,...

Osteosarcoma is the most common primary malignant bone tumor in children and adolescents has also been associated with a high degree of malignancy enhanced metastatic capacity. Curcumin (CUR) well known for its anti-osteosarcoma activity. However, both demethoxycurcumin (DMC), bisdemethoxycurcumin (BDMC) are natural curcumin analogues/congeners from turmeric whose role osteosarcoma development remains unknown.To evaluate growth inhibitory effects CUR, DMC BDMC on (HOS U2OS), breast...

To investigate the impact of both timing and dose rehabilitation delivery on functional recovery stroke patients.From chart review, we included 76 patients who were admitted to a regional hospital for first-ever stroke, had received multidisciplinary programs including physical therapy (PT) occupational (OT) at inpatient department, continuous outpatient department least 3 months. The collected data age, sex, type (hemorrhage/infarction), onset initial motor status by Brunnstrom's stages,...

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for disease 2019 (COVID-19). Since its emergence, the COVID-19 pandemic has not only distressed medical services but also caused economic upheavals, marking urgent need effective therapeutics. The experience of combating SARS-CoV and MERS-CoV shown that inhibiting 3-chymotrypsin-like protease (3CLpro) blocks replication virus. Given well-studied properties FDA-approved drugs, identification SARS-CoV-2 3CLpro...

Background and Purpose Non‐alcoholic fatty liver disease (NAFLD), including non‐alcoholic steatohepatitis, is closely related to metabolic diseases such as obesity diabetes. Despite an accumulating number of studies, no pharmacotherapy that targets NAFLD has received general approval for clinical use. Experimental Approach Inhibition the sodium‐glucose cotransporter 2 (SGLT2) a promising approach treat diabetes, obesity, associated disorders. In this study, we investigated effect novel SGLT2...

Avicennia marina is the most abundant and common mangrove species has been used as a traditional medicine for skin diseases, rheumatism, ulcers, smallpox. However, its anticancer activities polyphenol contents remain poorly characterized. Thus, here we investigated of secondary A. metabolites that were purified by sequential soxhlet extraction in water, ethanol, methanol, ethyl acetate (EtOAc). Experiments performed three human breast cancer cell lines (AU565, MDA-MB-231, BT483), two liver...

Breast cancer is the most universal in women, but medications for breast usually cause serious side effects and offer no effective treatment triple-negative cancer. Here, we investigated growth inhibitory of gallic acid (GA), (−)-epigallocatechin gallate (EGCG), or 1,2,3,4,6-penta-O-galloyl-β-d-glucose (5GG) combined with quercetin (Que) on cells. In this study, tested these compounds estrogen receptor (ER)/human epidermal factor 2 (Her2)-negative (MDA-MB-231), ER-positive/Her2-negative...

Chondrosarcoma is the second most common type of bone cancer. Surgical resection best choice for clinical treatment. High-grade chondrosarcoma destructive and more possible to metastasis, which difficult remove using surgery. Doxorubicin (Dox) commonly used chemotherapy drug in setting; however, resistance a major obstacle effective In present study, we compared Dox-resistant SW1353 cells their parental RNA sequencing (RNA-Seq). We found that apelin (APLN) pathway was highly activated...

Glioblastoma multiforme (GBM) is the most aggressive type of malignant brain tumor. Currently, predominant clinical treatment combination surgical resection with concurrent radiotherapy and chemotherapy, using temozolomide (TMZ) as primary chemotherapy drug. Lidocaine, a widely used amide‑based local anesthetic, has been found to have significant anticancer effect. It reported that aberrant hepatocyte growth factor (HGF)/mesenchymal‑epithelial transition (MET) signaling plays role in...

Nonalcoholic fatty liver disease (NAFLD) is common worldwide and closely associated with metabolic dysfunction. NAFLD leads to a higher risk of development severe diseases, such as nonalcoholic steatohepatitis (NASH), cirrhosis, hepatocellular carcinoma (HCC). To date, no pharmacotherapy targeting has received general approval. Adlay plant that been used traditional herbal medicine in Asia promising candidate solve this global issue. We have established mouse model by feeding high‐fat diet...

Obesity and associated conditions, such as type 2 diabetes mellitus (T2DM) nonalcoholic fatty liver disease (NAFLD), are currently a worldwide health problem. In Asian traditional medicine, Bai-Hu-Jia-Ren-Shen-Tang (BHJRST) is widely used in patients to reduce thirst. However, whether it has therapeutic effect on T2DM or NAFLD not known. The aim of this study was examine BHJRST had lipid-lowering using HuS-E/2 cell model induced by palmitate db/db mouse dyslipidemia. Incubation cells with...

Dietary fat has been suggested to be the cause of various health issues. Obesity, hypertension, cardiovascular disease, diabetes, dyslipidemia, and kidney disease are known associated with a high-fat diet (HFD). Obesity conditions, such as type 2 diabetes mellitus nonalcoholic fatty liver (NAFLD), currently worldwide problem. Few prospective pharmaceutical therapies that directly target NAFLD available at present. A Traditional Chinese Medicine, ginseng-plus-Bai-Hu-Tang (GBHT), is widely...

Persistent hepatitis B virus (HBV) infection causes liver cirrhosis and hepatocellular carcinoma constitutes a major worldwide health problem. Currently, anti-HBV drugs are limited to peginterferon nucleos(t)ide analogs, which costly have considerable side effects; the development of novel, effective agents is crucial.Catechins group compounds found in green tea extract epigallocatechin gallate (EGCG) has been shown antiviral properties, including inhibition cellular entry by HBV. FRG...

Obesity is a global health challenge that causes metabolic dysregulation and increases the risk of various chronic diseases. The gut microbiome crucial in modulating host energy metabolism, immunity, inflammation influenced by dietary factors. Gac fruit (Momordica cochinchinensis), widely consumed Southeast Asia, has been proven to have biological activities. However, composition effect crude gac aril polysaccharides (GAP) on obesity microbiota disturbed high-fat diet (HFD) remain be...

In this study, we aimed to develop a Stigmata Maydis (corn silk) fraction with dual bio-activities against oxidative stress and protein glycation protect β-cells from diabetes-induced failure.Corn silk fractions were prepared by partition chemically characterised thin-layer chromatography. Free radical scavenging assay, cell-based viability test (neutral red) employed decide the best fraction. Cell death analysis was executed annexin V/ Propidium iodide staining. proliferation measured...

Obesity and its associated health conditions, type 2 diabetes mellitus (T2DM) nonalcoholic fatty liver disease (NAFLD), are worldwide problems. It has been shown that insulin resistance is with increased hepatic lipid causes steatosis through a myriad of mechanisms, including inflammatory signaling.Helminthostachys zeylanica (HZ) used widely as common herbal medicine to relieve fever symptoms diseases in Asia. In the present study, we evaluated whether HZ therapeutic effects on obesity,...