A5044275001- Cancer-related Molecular Pathways
- Ovarian cancer diagnosis and treatment
- Microtubule and mitosis dynamics
- Cancer, Lipids, and Metabolism
- Cervical Cancer and HPV Research
- Epigenetics and DNA Methylation
- Gestational Trophoblastic Disease Studies
- Prenatal Screening and Diagnostics
- Cancer Research and Treatments
- Estrogen and related hormone effects
- interferon and immune responses
- Cancer Mechanisms and Therapy
- Virus-based gene therapy research
- Hippo pathway signaling and YAP/TAZ
- Molecular Biology Techniques and Applications
- RNA modifications and cancer
- Cell death mechanisms and regulation
- Wnt/β-catenin signaling in development and cancer
- Cytokine Signaling Pathways and Interactions
- FOXO transcription factor regulation
- Hedgehog Signaling Pathway Studies
- PARP inhibition in cancer therapy
- MicroRNA in disease regulation
- Hepatitis B Virus Studies
- Metabolism, Diabetes, and Cancer
University of Hong Kong
2012-2024
Chinese University of Hong Kong
2008-2024
Queen Mary Hospital
2011-2019
University of Hong Kong - Shenzhen Hospital
2018
Universiti Sains Malaysia
2015
Tsan Yuk Hospital
2012
Prostate Cancer Research
2007-2012
University College London
2007-2012
Ludwig Cancer Research
2011
Imperial College London
2011
Ovarian cancer is a lethal gynecological malignancy, and to improve survival, it important identify novel prognostic therapeutic targets. In this study, we present role for p21-activated kinase 4 (Pak4) in ovarian progression. We show significant association between increased expression of Pak4 its activated form, phosphorylated (p)-Pak4 Ser 474 , with metastasis cancers, shorter overall disease-free advanced stage high-grade serous/clear cell histological subtypes, reduced chemosensitivity....
Aim Deregulation of FOXM1 has been documented in various cancers. The aim this study was to evaluate the role ovarian cancer tumorigenesis and paclitaxel resistance. Experimental Design Expression examined 119 clinical samples by immunohistochemistry correlated with clinicopathological parameters. Effects knockdown on cell migration, invasion mitotic catastrophe were also studied. qPCR ChIP-qPCR used establish KIF2C as a novel target gene implicated chemoresistance. Results High nuclear...
Recent evidence has suggested that AMPK activators may be applied as therapeutic drugs in suppressing cancer cell growth. However, the molecular mechanism of their suppressive function cells is still unclear. Here we show impair cervical growth through reduction DVL3, a positive regulator Wnt/β-catenin signaling and an oncogenic player tumorigenesis. By western blot immunohistochemical analyses, demonstrated DVL3 was frequently upregulated significantly associated with elevated β-catenin (P...
Semaphorins are a large class of secreted or membrane-associated proteins that act as chemotactic cues for cell movement via their transmembrane receptors, plexins. We hypothesized the function semaphorin signaling pathway in control migration could be harnessed by cancer cells during invasion and metastasis. now report 13 somatic missense mutations cytoplasmic domain Plexin-B1 gene. Mutations were found 89% (8 9) prostate bone metastases, 41% (7 17) lymph node 46% (41 89) primary cancers....
iASPP is a specific regulator of p53-mediated apoptosis. Herein, we provided the first report on expression profile in ovarian epithelial tumor and its effect paclitaxel chemosensitivity.Expression amplification status was examined 203 clinical samples 17 cell lines using immunohistochemistry, quantitative real-time PCR, immunoblotting, correlated with clinicopathologic parameters. Changes proliferation, mitotic catastrophe, apoptosis, underlying mechanism cancer cells different p53...
Abstract Gene expression profiling aimed at classifying and prognosing breast cancer has yielded signatures with little, if any, concordance. However, arrays used in these studies do not discriminate alternate RNA splice isoforms that vary widely may resolve this problem. In study, we profiled a panel of tamoxifen-sensitive -resistant cell lines, defining novel variant (BQ323636.1) the nuclear receptor corepressor 2 (NCOR2) was associated tamoxifen resistance. Overexpression line induced its...
Ovarian clear cell carcinoma (OCCC) is a type of epithelial ovarian cancer that strongly associated with endometriosis, resistance against conventional chemotherapy and thus poorer prognosis. The expression inhibitory member the ASPP family proteins (iASPP) Polo-like kinase (PLK)1 were significantly higher in OCCC compared to benign cystadenomas endometriosis. Both protein expressions found correlate chemoresistance patients while high iASPP alone was poor patient survival. growth lines,...
Gestational choriocarcinoma is a malignant tumor derived from placental trophoblast and the most aggressive member of gestational trophoblastic disease (GTD). Apoptosis-stimulating protein p53-2 (ASPP2) ASPP family that transactivates p53 thereby functions as suppressor. In this study, expression profile ASPP2 in was examined comparison with normal placentas hydatidiform moles, latter being type GTD carries potential. Downregulation messenger RNA demonstrated by quantitative PCR...
This paper aimed at investigating the expression and methylation profiles of SOX2 , a gene coding for stem cell‐related transcription factor in endometrial carcinomas. By methylation‐specific polymerase chain reaction (MS‐PCR), status promoter region 72 carcinomas 12 normal samples was examined. Methylated allele found 37.5% (27/72) but only 8.3% (1/12) endometrial, significantly more frequent cancers ( P = .0472). mRNA level reduced carcinoma compared with nonneoplastic endometrium .045). A...
Zhao F, Siu M K Y, Jiang L L, Tam Ngan H Y S, Le X-F, Wong O G W, E S Chan & Cheung A N (2011) Histopathology 59, 1163–1172 Overexpression of dedicator cytokinesis I (Dock180) in ovarian cancer correlated with aggressive phenotype and poor patient survival Aims: Dedicator is a novel guanine nucleotide exchange factor for Rho guanosine triphosphates (GTPases) important cell migration. The aim this study was to evaluate the role Dock180 carcinogenesis. Methods results: Using...
The kinesin protein Kif7 has been recognized as an integral component of hedgehog signalling. Aberrant activation signalling implicated in many human solid tumours. Gestational trophoblastic disease includes frankly malignant choriocarcinoma and potentially hydatidiform mole. Here we investigated the components expression profiles gestational disease. Downregulation Gli1, Gli2, Gli3 was demonstrated clinical samples moles well cell lines when compared with normal placentas. Ectopic two JAR...
Abstract This study tried to evaluate the application of a novel cancer gene therapy using recombinant adeno-associated virus (AAV) carrying kringle 1 domain human hepatocyte growth factor (AAV-HGFK1) in combination with adenovirus p53 (Ad-p53). BALB/c and nude mice models colon were established treated AAV-HGFK1 alone or Ad-p53. Combination Ad-p53 significantly prolonged survival also inhibited primary secondary tumor growth. Histochemical examination tumors revealed that AAV-HGFK1+Ad-p53...
High-risk human papillomavirus (HR-HPV) DNA detection in cervical cytology samples is useful for primary screening of cancer and triage patients with equivocal cytological findings. The GenoFlow HPV array test (GF assay; Diagcor Bioscience Inc., Hong Kong) was recently developed to detect 33 genotypes by a "flowthrough" hybridization technology. In this study, we assessed the analytical sensitivity reproducibility GF assay compared its genotyping results those Linear Array (LA) (Roche...
Abstract Background Semaphorins act as chemotactic cues for cell movement via their transmembrane receptors, plexins. Somatic missense mutations in the plexinB1 gene coupled with overexpression of protein frequently occur prostate tumours, indicating a role pathogenesis cancer. Results Two specific found cancer enhance RhoD binding and one other mutation results loss inhibition Rac-dependent Pak1 phosphorylation lamellipodia formation impairment trafficking to membrane. None three...
The Arias-Stella reaction (ASR) can mimic endometrial clear cell carcinoma (ECCC) in small biopsies, especially when drug or pregnancy history is unknown. A panel of immunohistochemical markers comprising napsin A, hepatocyte nuclear factor-1-beta (HNF-1β), estrogen and progesterone receptors (ER, PR) has been found useful confirming a diagnosis ECCC. However, the detailed characterization how expression this combination ECCC mimics ASR yet to be thoroughly evaluated.The frequency extent...
This study aimed (i) to compare the performance of BD Onclarity human papillomavirus (HPV) assay with Cobas HPV test in identifying cervical intraepithelial neoplasia 2/3 or above (CIN2/3+) an Asian screening population and (ii) explore improving cancer detection specificity by machine learning. We tested 605 stratified random archived samples liquid-based cytology both assays. All had biopsy diagnosis repeated negative follow-up. Association rule mining (ARM) was employed discover...