A5014184670- Trace Elements in Health
- Iron Metabolism and Disorders
- Hemoglobinopathies and Related Disorders
- Heavy Metal Exposure and Toxicity
- Reproductive Biology and Fertility
- Heme Oxygenase-1 and Carbon Monoxide
- Sperm and Testicular Function
- Drug Transport and Resistance Mechanisms
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Erythrocyte Function and Pathophysiology
- Neonatal Health and Biochemistry
- Animal Genetics and Reproduction
- Genetic Mapping and Diversity in Plants and Animals
- RNA regulation and disease
- Ovarian function and disorders
- Renal and related cancers
- Genomics and Chromatin Dynamics
- Genetic and Kidney Cyst Diseases
- Biomedical Research and Pathophysiology
- Inflammasome and immune disorders
- Viral Infections and Immunology Research
- Electrolyte and hormonal disorders
- Neurological Complications and Syndromes
- Pharmacological Effects and Assays
- Testicular diseases and treatments
Jagiellonian University
2015-2024
Aging affects iron homeostasis, as evidenced by tissue loading and anemia in the elderly. Iron needs mammals are met primarily recycling from senescent red blood cells (RBCs), a task chiefly accomplished splenic pulp macrophages (RPMs) via erythrophagocytosis. Given that RPMs continuously process iron, their cellular functions might be susceptible to age-dependent decline, possibility has been unexplored date. Here, we found 10- 11-month-old female mice exhibit RPMs, largely attributable...
Heme is an efficient source of iron in the diet, and heme preparations are used to prevent cure deficiency anemia humans animals. However, molecular mechanisms responsible for absorption remain only partially characterized. Here, we employed young iron-deficient piglets as a convenient animal model determine efficacy oral supplementation investigate pathways absorption. The use bovine hemoglobin dietary was found efficiently counteract development piglets, although it did not fully rebalance...
Menkes Disease (MD) is a rare X-linked recessive fatal neurodegenerative disorder caused by mutations in the ATP7A gene, and most patients are males. Female carriers mosaics of wild-type mutant cells due to random X inactivation, they rarely affected. In largest cohort MD reported so far which consists 517 families we identified 9 neurologically affected with normal karyotypes.We investigated at-risk females for gene sequencing or multiplex ligation-dependent probe amplification (MLPA). We...
Several recent studies have indicated that androgen disruption induced by the administration of anti-androgen flutamide during critical developmental stages results in various reproductive abnormalities, mainly rodents. However, scarce data are available regarding alterations caused this toxicant on cell-cell adhesion molecules. Of note, there is no report expression and regulation tight adherens junction proteins boar. Therefore, purpose study was to analyse whether foetal neonatal exposure...
HO1 (haem oxygenase 1) and Fpn (ferroportin) are key proteins for iron recycling from senescent red blood cells therefore play a major role in controlling the bioavailability of erythropoiesis. Although important aspects metabolism HO1-deficient (Hmox1-/-) mice have already been revealed, little is known about regulation expression its deficiency. In present study, we characterize cellular systemic factors influencing Hmox1-/- bone marrow-derived macrophages liver kidney mice. macrophages,...
Abstract Background The critically low hepatic iron stores of newborn piglets are considered to be a major cause neonatal deficiency in modern breeds domestic pig ( Sus domestica ). main factor believed contribute this phenomenon is large litter size, which has been an objective selective breeding pigs for decades. As consequence, transferred from the pregnant sow distributed among greater number fetuses. Results Here, we investigated whether size influences red blood cell (RBC) indices and...
The demand for iron is high in pregnancy to meet the increased requirements erythropoiesis. Even pregnant females with initially iron-replete stores develop iron-deficiency anemia, due inadequate absorption. In anemic females, maternal supply dedicated maintaining metabolism fetus and placenta. Here, using a mouse model of deficiency pregnancy, we show that recycled from senescent erythrocytes becomes predominant source this microelement can be transferred placenta depleted stores....
In the present study, animals with a genetic defect in copper metabolism were used as model organism to study role of reproduction and determine whether disturbances zinc affect testicular tissue gamete quality males. Mice an X-linked mosaic mutation (Atp7amo-ms) exhibit pathological features characteristic affected metabolism. This usually leads lethality mutant males which generally expire on about day 16. Only 4% survive critical period, achieve maturity, become fertile. To improve...
Neutrophil extracellular traps (NETs) contribute to pathological disorders and their release was directly linked numerous diseases. With intravital microscopy (IVM) we showed previously that NETs also the pathology of systemic inflammation are strongly deposited in liver sinusoids. Over a decade since NET discovery still not much is known about metabolic or microenvironmental aspects formation. Copper vital trace element essential for many biological processes, albeit its excess potentially...
Copper and iron metabolism have been known to interact for many years. We previously shown, during pregnancy, that copper levels in the maternal liver rise as a consequence of deficiency, but fetal decrease. In this paper, we measure expression genes involved postnatal liver, test whether alterations can explain observation. Additionally, study extent which gene changes latter stages pregnancy perinatal period. Ctr1 dropped term, rising again thereafter. There was no difference between...
Among livestock, domestic pig (Sus scrofa) is a species, in which iron metabolism has been most intensively examined during last decade. The obvious reason for studying the regulation of homeostasis especially young pigs neonatal deficiency anemia commonly occurring these animals. Moreover, supplementation essentially all commercially reared piglets with entails need monitoring efficacy this routine practice followed swine industry several decades. Since discovery hepcidin many studies...
Amyotrophic lateral sclerosis (ALS) is a progressive neurodegenerative disease characterized by degeneration and loss of motor neurons in the spinal cord, brainstem cortex. Up to 10% ALS cases are inherited (familial, fALS) associated with mutations, frequently superoxide dismutase 1 (SOD1) gene. Rodent transgenic models often used elucidate complex pathogenesis this disease. Of importance, both patients animals carrying mutated human SOD1 gene show symptoms oxidative stress iron metabolism...
In all living organisms trace element metabolism and transport are closely regulated at the genetic level. Copper is one of essential microelements required for normal growth development. The main organ in mammals involved copper liver. It known that liver controlled by ATP7B, a P-type ATP-ase encoded Atp7b gene. However, little about expression function second important ATP-ase, ATP7A Atp7a this study we investigated gene during postnatal development mice. We analyzed livers from neonatal...
The maintenance of copper homeostasis is critical for all cells. As learned from mice with disturbed metabolism, this trace element also important spermatogenesis. experiments conducted in yeasts have demonstrated that appropriate level must be preserved to enable meiosis progression; however, increased toxic This study aims analyze the expression profile Atp7a and Atp7b other genes encoding copper-related proteins during spermatogenesis mice. Using transcripts protein detection techniques,...
Jackson toxic milk mutant mice (tx-J) carrying a missense mutation in the Atp7b gene are animal models of Wilson disease. In both patients and tx-J mice, mutations ATP7B/Atp7b lead to disturbances copper metabolism. The dysfunction leads reduction incorporation into apoceruloplasmin; this decreases ferroxidase activity ceruloplasmin necessary for efflux iron from cells reduces release hepatocytes bile; results massive hepatic accumulation. A decrease emphasises practicality model exploration...
In pigs, iron deficiency anemia (IDA) is the most prevalent disorder during early postnatal period frequently developing into a critical illness. Meanwhile, in humans, only low-birth-weight infants, including premature infants are especially susceptible to IDA. both human and pig neonates, initial cause of IDA low birth stores. piglets this shortage stored results mainly from genetic selection over past few decades for large litter size high weight. consequence, pregnant sows cannot provide...
Neonatal iron deficiency anemia is prevalent among domestic pigs but does not occur in the offspring of wild boar. The main causes this disorder piglets modern pig breeds are paucity hepatic stores, high birth weight, and rapid growth. Replenishment fetal stores a direct result transfer efficiency across placenta. In study, we attempted to investigate molecular potential placenta as possible cause differences between boar Polish Large White (PLW) offspring. Furthermore, by analyzing...