A5083541113- Metabolomics and Mass Spectrometry Studies
- Organophosphorus compounds synthesis
- Metal complexes synthesis and properties
- Chemical Synthesis and Analysis
- Chemical Synthesis and Characterization
- DNA and Nucleic Acid Chemistry
- Gut microbiota and health
- Molecular Sensors and Ion Detection
- Photochromic and Fluorescence Chemistry
- Crystallization and Solubility Studies
- Diet and metabolism studies
- Advanced Chemical Sensor Technologies
- Advanced biosensing and bioanalysis techniques
- X-ray Diffraction in Crystallography
- Metabolism and Genetic Disorders
- Synthesis and Reactivity of Sulfur-Containing Compounds
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Protein Interaction Studies and Fluorescence Analysis
- Bone health and treatments
- RNA and protein synthesis mechanisms
- Liver Disease Diagnosis and Treatment
- Microbial Fuel Cells and Bioremediation
- Phosphorus compounds and reactions
- Supramolecular Self-Assembly in Materials
- Thyroid Cancer Diagnosis and Treatment
Wrocław University of Science and Technology
2016-2025
AGH University of Krakow
2016-2024
Wroclaw Medical University
2014-2023
Max Planck Institute of Biochemistry
2023
Ludwik Hirszfeld Institute of Immunology and Experimental Therapy
2023
Polish Academy of Sciences
2023
Faculty (United Kingdom)
2016-2018
University of Wrocław
2000-2006
University of Gdańsk
2004
Institute of Organic Chemistry
2004
AIM:To evaluate the utility of serum and urine metabolomic analysis in diagnosing monitoring inflammatory bowel diseases (IBD). METHODS:Serum samples were collected from 24 patients with ulcerative colitis (UC), 19 Crohn's disease (CD) 17 healthy controls.The activity UC was assessed Simple Clinical Colitis Activity Index, while CD determined using Harvey-Bradshaw Index.The performed proton nuclear magnetic resonance (NMR) spectroscopy.All spectra exported to Matlab for preprocessing which...
Metabolic profiles of amniotic fluid and maternal blood are sources valuable information about fetus development can be potentially useful in diagnosis pregnancy disorders. In this study, we applied 1H NMR-based metabolic profiling to track changes occurring (AF) plasma (PL) healthy mothers over the course pregnancy. AF PL samples were collected 2nd (T2) 3rd (T3) trimester, prolonged (PP) until time delivery (TD). A multivariate data analysis both biofluids reviled a switch-like transition...
Irritable bowel syndrome (IBS) is a chronic functional disorder and its development may be linked, directly indirectly, to intestinal dysbiosis. Here we investigated the interactions between IBS symptoms gut microbiome, including relation rifaximin (1200 mg daily; 11.2 g per treatment). We recruited 72 patients, 31 with IBS-D (diarrhea), 11 IBS-C (constipation), 30 IBS-M (mixed constipation diarrhea) healthy controls (HCs). Of them, 68%, 64%, 53% patients IBS-D, IBS-C, IBS-M, respectively,...
Thyroid cancer is the most common endocrine malignancy. However, more than 90% of thyroid nodules are benign. It remains unclear whether carcinoma arises from preexisting benign nodules. Metabolomics can provide valuable and comprehensive information about low molecular weight compounds present in living systems further our understanding biology regulating pathological processes. Herein, we applied 1H NMR-based metabolic profiling to identify metabolites aqueous tissue extracts healthy (H),...
Rheumatoid arthritis is a chronic autoimmune-based inflammatory disease that leads to progressive joint degeneration, disability, and an increased risk of cardiovascular complications, which the main cause mortality in this population patients. Although several biomarkers are routinely used management rheumatoid arthritis, there high demand for novel further improve early diagnosis stratification patients, prediction better response specific therapy. In study, metabolomics approach was...
Abstract Early detection of nodular thyroid diseases including cancer is still primarily based on invasive procedures such as fine-needle aspiration biopsy. Therefore, there a strong need for development new diagnostic methods that could provide clinically useful information regarding lesions in non-invasive way. In this study we investigated 1 H NMR metabolic profiles paired urine and blood serum samples, were obtained from healthy individuals patients with diseases. Estimation predictive...
Abstract The photochromic properties of azobenzene, involving conformational changes occurring upon interaction with light, provide an excellent tool to establish new ways selective regulation applied biosystems. We report here on the binding two water-soluble 4-(phenylazo)benzoic acid derivatives (Azo-2N and Azo-3N) double stranded DNA demonstrate that photoisomerization Azo-3N leads in structure. In particular, we show stabilization destabilization B-DNA secondary structure can be...
Burkitt lymphoma (BL) is a rapidly growing tumor, characterized by high anabolic requirements. The MYC oncogene plays central role in the pathogenesis of this malignancy, controlling genes involved apoptosis, proliferation, and cellular metabolism. Serine biosynthesis pathway (SBP) couples glycolysis to folate methionine cycles, supporting certain amino acids, nucleotides, glutathione, methyl group donor, S-adenosylmethionine (SAM). We report that BLs overexpress SBP enzymes,...
Glucose is a predominant fuel for the brain supporting its high energy demand associated with neuronal signaling and synaptic activity. Long-term potentiation (LTP) required learning memory formation by generating long lasting increase in strength signal transmission between two neurons. While electrophysiological bases of LTP are well established, much less known about metabolic demands neurons involved LTP. Common protocols used to examine activity rely on glucose concentrations which far...
Chronic obstructive pulmonary disease, COPD, affects the condition of entire human organism and causes multiple comorbidities. Pathological lung changes lead to quantitative in composition metabolites different body fluids. The sleep apnea syndrome, OSAS, occurs conjunction with chronic disease about 10-20 % individuals who have COPD. Both conditions share same comorbidities this makes differentiating them difficult. aim study was investigate whether it is possible diagnose a patient either...
Chronic periodontitis is an inflammatory disease of tooth-supporting tissues associated with Porphyromonas gingivalis. Expansion and invasion this bacterium into the periodontium changes in metabolome oral cavity. Materials Methods: Metabolomics analysis mouth washout tongue swab samples based on proton nuclear magnetic resonance (<sup>1</sup>H-NMR) method was employed to determine metabolic status cavity chronic periodontal disease. Results: Mouth extracts contained a total 23 metabolites...
Carrot seed oil is the source of carotane sesquiterpenes carotol, daucol and β-caryophyllene. These sesquiterpenic allelochemicals were evaluated against Alternaria alternata isolated from surface carrot seeds cultivar Perfekcja, a variety widely distributed in horticultural practise Poland. one most popular phytotoxic fungi infesting plant. The strongest antifungal activity was observed for main constituent oil, which inhibited radial growth by 65% at following concentration.
Seed coating containing fertilizer nutrients and plant growth biostimulants is an innovative technique for precision agriculture. Nutrient delivery can also be conducted through multilayer seed coating. For this purpose, sodium alginate with NPK, which was selected in a preliminary selection study, crosslinked divalent ions (Cu(II), Mn(II), Zn(II)) as source of micronutrients, used to produce The seeds were additionally coated solution amino acids derived from high-protein material. Amino...
Abstract Systemic metabolic changes after renal transplantation reflect the key processes that are related to graft accommodation. In order describe and better understand these changes, 1 HNMR based metabolomics approach was used. The of 47 metabolites in serum samples 19 individuals were interpreted over time with respect their levels prior transplantation. Considering specific repeated measures design experiments, data analysis mainly focused on multiple analyses variance (ANOVA) methods...
Non-alcoholic fatty liver disease (NAFLD) is associated with dysfunction of the intestinal microbiota and its metabolites. We aimed to assess whether replacing bread high-fiber buns beneficially changes metabolome in NAFLD patients. This study involved 27 adult patients validated by FibroScan® (CAP ≥ 234 dB/m). Patients were asked replace their existing for two meals buns. In this way, ate rolls every day 2 months. The following parameters analysed (at beginning after months): anthropometric...