A5064634385- Mitochondrial Function and Pathology
- Cancer, Hypoxia, and Metabolism
- Wnt/β-catenin signaling in development and cancer
- Neuroscience and Neuropharmacology Research
- Metabolism, Diabetes, and Cancer
- Endoplasmic Reticulum Stress and Disease
- PI3K/AKT/mTOR signaling in cancer
- Genetics and Neurodevelopmental Disorders
- Advanced Proteomics Techniques and Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Cancer Cells and Metastasis
- Hedgehog Signaling Pathway Studies
- Alzheimer's disease research and treatments
- Cellular transport and secretion
- Metabolomics and Mass Spectrometry Studies
- Cancer-related Molecular Pathways
- Cancer-related gene regulation
- Autophagy in Disease and Therapy
- Nuclear Receptors and Signaling
- RNA modifications and cancer
- Ubiquitin and proteasome pathways
- MicroRNA in disease regulation
- RNA Research and Splicing
- 14-3-3 protein interactions
- Advances in Cucurbitaceae Research
University of Wrocław
2014-2025
Wrocław Zoo
2005
Abstract Lactate derived from astrocytic glycogen has been shown to support memory formation in hippocampi of young animals, inhibiting it old animals. Here we show, using quantitative mass spectrometry‐based proteomics, immunofluorescence, and qPCR that aging is associated with an increase metabolism enzymes concentration shift their localization astrocytes neurons. These changes are accompanied reorganization hippocampal energy which manifested by elevated capacity neurons oxidize glucose...
Glucose is a predominant fuel for the brain supporting its high energy demand associated with neuronal signaling and synaptic activity. Long-term potentiation (LTP) required learning memory formation by generating long lasting increase in strength signal transmission between two neurons. While electrophysiological bases of LTP are well established, much less known about metabolic demands neurons involved LTP. Common protocols used to examine activity rely on glucose concentrations which far...
Fructose-1,6-bisphosphatase (FBPase) catalyzes the hydrolysis of fructose 1,6-bisphosphate to 6-phosphate and is a key enzyme gluconeogenesis glyconeogenesis and, more generally, control energy metabolism glucose homeostasis. Vertebrates, notably Homo sapiens, express two FBPase isoforms. The liver isozyme expressed mainly in gluconeogenic organs, where it functions as regulator synthesis. muscle isoform all cells, recent studies have demonstrated that its role goes far beyond enzymatic...
Recent studies have revealed a resemblance of HIF-regulated heart and brain glycolytic profiles prompting the hypothesis that classical cell-to-cell lactate shuttle observed between astrocytes neurons operates also in -between cardiac fibroblasts cardiomyocytes.Here, we demonstrate coculturing cardiomyocytes with leads to orchestrated changes expression and/or localization pattern glucose metabolism enzymes transport proteins both cell types.These are regulated by paracrine signaling using...
Glycogen synthase kinase-3 (GSK-3) is a regulator of signaling pathways. KRas frequently mutated in pancreatic cancers. The growth certain cancers KRas-dependent and can be suppressed by GSK-3 inhibitors, documenting link between GSK-3. To further elucidate the roles GSK-3β drug-resistance, we transfected MIA-PaCa-2 cells with wild-type (WT) kinase-dead (KD) forms GSK-3β. Transfection WT-GSK-3β increased their resistance to various chemotherapeutic drugs small molecule inhibitors. KD-GSK-3β...
FBPase physically interacts with ATP synthase subunit β, α, ADP/ATP translocase 1, 2 and VDAC2 by cross‐linking study (View interaction)
Fibroblasts are important contributors to cancer development. They create a tumor microenvironment and modulate our metabolism treatment resistance. In the present paper, we demonstrate that healthy fibroblasts induce metabolic coupling with non-small cell lung cells by down-regulating expression of glycolytic enzymes in increasing fibroblasts' ability release lactate thus support energy-rich glucose-derived metabolites, such as pyruvate-a process known reverse Warburg effect. We these...
The TP53 tumor suppressor is mutated in ~75% of pancreatic cancers. mutant protein ductal adenocarcinomas (PDAC) promotes growth and metastasis. Attempts have been made to develop molecules that restore at least some the properties wild-type (WT) TP53. APR-246 one such molecule, it referred as a reactivator. To understand potential sensitize PDAC cells chemotherapy, we introduced vector encoding WT-TP53 into two cell lines, lacking expression (PANC-28) with gain-of-function (GOF)...
It has been shown that neurons alter the expression of astrocytic metabolic enzymes by secretion until now unknown molecule(s) into extracellular fluid. Here, we present evidence neuron-derived transthyretin (TTR) stimulates glycolytic in astrocytes which is reflected an increased synthesis ATP. The action TTR restricted to regulatory glycolysis: phosphofructokinase P (PFKP) and pyruvate kinase M1/M2 isoforms (PKM1/2). regulation PFK PKM presumably specific for brain tissue independent role...
Total protein approach (TPA) is a proteomic method that allows calculation of concentrations individual proteins and groups functionally related in any mixture without spike-in standards. Using the two-step digestion-filter-aided sample preparation LC-MS/MS analysis, we generated comprehensive quantitative datasets mouse intestinal mucosa, liver, red muscle fibers, brain, human plasma, erythrocytes, tumor cells lines. We show TPA-based data reflect well-defined specific physiological...
Aging is believed to be the result of alterations protein expression and accumulation changes in biomolecules. Although there are numerous reports demonstrating brain during aging, only few them describe global at level. Here, we present deepest quantitative proteomic analysis three regions, hippocampus, cortex cerebellum, mice aged 1 or 12 months, using total approach technique. In all both young middle-aged animals, quantitatively measured over 5,200 proteins. We found that although...
It has been recently found that muscle fructose 1,6-bisphosphatase (FBPase) is actively transported into cells' nuclei. Results of an analysis in silico FBPase structure gave rise to a hypothesis sequence (203)KKKGK(207) responsible for nuclear targeting the enzyme. To test this, HL-1 cardiomyocytes were transfected with FITC-labeled constructs, bearing mutations within putative localization signal (NLS). revealed integrity motif critical and even single amino-acid change this results...
Long-term potentiation (LTP) is a molecular basis of memory formation. Here, we demonstrate that LTP critically depends on fructose 1,6-bisphosphatase 2 (Fbp2)—a glyconeogenic enzyme and moonlighting protein protecting mitochondria against stress. We show induction regulates Fbp2 association with neuronal Camk2 the Fbp2–Camk2 interaction correlates autophosphorylation. Silencing expression or simultaneous inhibition tetramerization synthetic effector mimicking action physiological inhibitors...