A5038928679- Mitochondrial Function and Pathology
- Cancer, Hypoxia, and Metabolism
- Metabolism, Diabetes, and Cancer
- Wnt/β-catenin signaling in development and cancer
- Cancer-related Molecular Pathways
- PI3K/AKT/mTOR signaling in cancer
- Endoplasmic Reticulum Stress and Disease
- Hedgehog Signaling Pathway Studies
- Neuroscience and Neuropharmacology Research
- Berberine and alkaloids research
- Genetic Neurodegenerative Diseases
- Ubiquitin and proteasome pathways
- Muscle Physiology and Disorders
- Genetics and Neurodevelopmental Disorders
- Pancreatic and Hepatic Oncology Research
- Autophagy in Disease and Therapy
- Cancer Cells and Metastasis
- Glycogen Storage Diseases and Myoclonus
- Muscle metabolism and nutrition
- Advanced Proteomics Techniques and Applications
- Epigenetics and DNA Methylation
- Metabolomics and Mass Spectrometry Studies
- RNA modifications and cancer
- Biotin and Related Studies
- Bioactive natural compounds
University of Wrocław
2016-2025
Wrocław Zoo
2001-2008
Abstract Lactate derived from astrocytic glycogen has been shown to support memory formation in hippocampi of young animals, inhibiting it old animals. Here we show, using quantitative mass spectrometry‐based proteomics, immunofluorescence, and qPCR that aging is associated with an increase metabolism enzymes concentration shift their localization astrocytes neurons. These changes are accompanied reorganization hippocampal energy which manifested by elevated capacity neurons oxidize glucose...
Abstract Muscle fructose-1,6-bisphosphate aldolase (ALDOA) is among the most abundant glycolytic enzymes in all cancer cells. Here, we show that enzyme plays a previously unknown and critical role cell survival. Simultaneous inhibition of ALDOA activity interaction with F-actin cytoskeleton using slow-binding inhibitor UM0112176 leads to rapid cofilin-dependent loss stress fibers which associated elevated ROS production, ATP synthesis, increase calcium levels, caspase activation arrested...
Astrocytes releasing glucose‐ and/or glycogen‐derived lactate and glutamine play a crucial role in shaping neuronal function plasticity. Little is known, however, how metabolic functions of astrocytes, e.g., their ability to degrade glucosyl units, are affected by the presence neurons. To address this issue we carried out experiments which demonstrated that co‐culturing rat hippocampal astrocytes with neurons significantly elevates level mRNA protein for enzymes glycolysis...
Slow and fast skeletal muscles are composed of, respectively, mainly oxidative glycolytic muscle fibers, which the basic cellular motor units of motility apparatus. They largely differ in excitability, contraction mechanism, metabolism. Because their pivotal role body motion homeostasis, have been extensively studied using biochemical molecular biology approaches. Here we describe a simple analytical computational approach to estimate titers enzymes metabolic pathways proteins contractile...
Glycolysis is the core metabolic pathway supplying energy to cells. Whereas vast majority of studies focus on specific aspects process, global analyses characterizing simultaneously all enzymes involved in process are scarce. Here, we demonstrate that quantitative label- and standard-free proteomics allows accurate determination titers enables simultaneous measurements maximal enzymatic activities (Amax) glycolytic gluconeogenic fructose 1,6-bisphosphatase mouse brain, liver muscle. Despite...
Recent studies have revealed a resemblance of HIF-regulated heart and brain glycolytic profiles prompting the hypothesis that classical cell-to-cell lactate shuttle observed between astrocytes neurons operates also in -between cardiac fibroblasts cardiomyocytes.Here, we demonstrate coculturing cardiomyocytes with leads to orchestrated changes expression and/or localization pattern glucose metabolism enzymes transport proteins both cell types.These are regulated by paracrine signaling using...
Glycogen synthase kinase-3 (GSK-3) is a regulator of signaling pathways. KRas frequently mutated in pancreatic cancers. The growth certain cancers KRas-dependent and can be suppressed by GSK-3 inhibitors, documenting link between GSK-3. To further elucidate the roles GSK-3β drug-resistance, we transfected MIA-PaCa-2 cells with wild-type (WT) kinase-dead (KD) forms GSK-3β. Transfection WT-GSK-3β increased their resistance to various chemotherapeutic drugs small molecule inhibitors. KD-GSK-3β...