A5052076867- Advanced Proteomics Techniques and Applications
- RNA Research and Splicing
- Biotin and Related Studies
- Mass Spectrometry Techniques and Applications
- RNA modifications and cancer
- Gene expression and cancer classification
- Genomics and Chromatin Dynamics
- RNA and protein synthesis mechanisms
- Genetic factors in colorectal cancer
- Cancer-related molecular mechanisms research
- Colorectal Cancer Screening and Detection
- Mitochondrial Function and Pathology
- Peptidase Inhibition and Analysis
- Advanced Biosensing Techniques and Applications
- Blood disorders and treatments
- Diet and metabolism studies
- Kruppel-like factors research
- Viral Infections and Immunology Research
- Autophagy in Disease and Therapy
- Ovarian cancer diagnosis and treatment
- Cellular transport and secretion
- Cholesterol and Lipid Metabolism
- Peroxisome Proliferator-Activated Receptors
- Gastric Cancer Management and Outcomes
- Bioinformatics and Genomic Networks
Warsaw University of Technology
2013-2024
The Maria Sklodowska-Curie National Research Institute of Oncology
2006-2011
Background Clinical progression of colorectal cancers (CRC) may occur in parallel with distinctive signaling alterations. We designed multidirectional analyses integrating microarray-based data biostatistics and bioinformatics to elucidate the metabolic alterations underlying CRC development adenoma-carcinoma sequence. Methodology/Principal Findings Studies were performed on normal mucosa, adenoma, carcinoma samples obtained during surgery or colonoscopy. Collections cryostat sections...
Histone post-translational modifications (PTMs) play an important role in the regulation of expression genes, including those involved cancer development and progression. However, our knowledge PTM patterns human tumours is limited.MS-based analyses were used to quantify global alterations histone PTMs colorectal (CRC) samples. Histones isolated from 12 CRCs their corresponding normal mucosa by acidic extraction separated SDS-PAGE analysed liquid chromatography-mass spectrometry.Among 96...
Irritable bowel syndrome (IBS) is a chronic functional disorder and its development may be linked, directly indirectly, to intestinal dysbiosis. Here we investigated the interactions between IBS symptoms gut microbiome, including relation rifaximin (1200 mg daily; 11.2 g per treatment). We recruited 72 patients, 31 with IBS-D (diarrhea), 11 IBS-C (constipation), 30 IBS-M (mixed constipation diarrhea) healthy controls (HCs). Of them, 68%, 64%, 53% patients IBS-D, IBS-C, IBS-M, respectively,...
The heterogeneous nuclear ribonucleoprotein K is an ancient RNA/DNA-binding protein that involved in multiple processes compose gene expression. pleiotropic action of reflects its ability to interact with different classes factors, interactions are regulated by extracellular signals. We used affinity purification and MS better define the repertoire partners. identified a large number new partners, some typically found subcellular compartments, such as plasma membrane, where has not...
Background: Clinical progression of colorectal cancers (CRC) may occur in parallel with distinctive signaling alterations.We designed multidirectional analyses integrating microarray-based data biostatistics and bioinformatics to elucidate the metabolic alterations underlying CRC development adenoma-carcinoma sequence.Methodology/Principal Findings: Studies were performed on normal mucosa, adenoma, carcinoma samples obtained during surgery or colonoscopy.Collections cryostat sections...
// Lukasz Michal Szafron 1, * , Anna Balcerak 2, Ewa Grzybowska 2 Barbara Pienkowska-Grela 1 Agnieszka Podgorska Renata Zub Magdalena Olbryt 3 Jolanta Pamula-Pilat Katarzyna M. Lisowska Tymon Rubel 4 Dansonka-Mieszkowska Bozena Konopka Kulesza Martyna Lukasik Kupryjanczyk Department of Pathology and Laboratory Diagnostics, Maria Sklodowska-Curie Memorial Cancer Center Institute Oncology, Warsaw, Poland Molecular Translational for Research Biology Cancer, Gliwice Branch, Radioelectronics...
CRNDE, recently described as the lncRNA-coding gene, is overexpressed at RNA level in human malignancies. Its role gametogenesis, cellular differentiation and pluripotency has been suggested well. Herein, we aimed to verify our hypothesis that CRNDE gene may encode a protein product, CRNDEP. By using bioinformatics methods, identified 84-amino acid ORF encoded by one of two transcripts, previously research team. This was cloned into expression vectors, subsequently utilized localization...
Abstract Mounting evidence indicates that MS analysis of the human blood peptidome allows to distinguish between cancer and non‐cancer samples, giving promise for a new MS‐based diagnostic tool. However, several aspects already published work have been criticized demand more methodical approach has formulated. Motivated by this we undertook systematic study plasma serum using an integrated ESI‐LC‐MS‐based platform, equipped with data tools relative absolute peptide quantitation. We used high...
Kinase domains are the type of protein domain most commonly found in genes associated with tumorigenesis. Because this, human kinome (the kinase component genome) represents a promising source cancer biomarkers and potential targets for novel anti-cancer therapies. Alterations colon during progression from normal (NC) through adenoma (AD) to adenocarcinoma (AC) were investigated using integrated transcriptomic proteomic datasets. Two hundred thirty 42 proteins showed differential expression...
Linker histones (H1s) are conserved and ubiquitous structural components of eukaryotic chromatin. Multiple non-allelic variants H1, which differ in their DNA/nucleosome binding properties, co-exist animal plant cells have been implicated the control genetic programs during development differentiation. Studies mammals Drosophila revealed diverse post-translational modifications H1s, most unknown function. So far, it is not known how this pattern compares with that H1s from other major...
Sortilin is a neuronal receptor for apolipoprotein E (apoE). Sortilin-dependent uptake of lipidated apoE promotes conversion polyunsaturated fatty acids (PUFA) into neuromodulators that induce anti-inflammatory gene expression in the brain. This neuroprotective pathway works with apoE3 variant but lost apoE4 variant, main risk factor Alzheimer's disease (AD). Here, we elucidated steps cellular handling lipids through sortilin, and why they are disrupted by apoE4. Combining unbiased proteome...
Definitive identification of promoters, their cis-regulatory motifs, and trans-acting proteins requires experimental analysis. To define the HNRNPK promoter its cognate DNA–protein interactions, we performed a comprehensive study combining approaches, including luciferase reporter gene assays, chromatin immunoprecipitations (ChIP), electrophoretic mobility shift assays (EMSA), mass spectrometry (MS). We discovered that out four potential promoters tested, one containing palindromic motif...
CRNDE is considered an oncogene expressed as long non-coding RNA. Our previous paper the only one reporting a micropeptide-coding gene. The amino acid sequence of this micropeptide (CRNDEP) has recently been confirmed by other researchers. This study aimed at providing mass spectrometry (MS)-based validation CRNDEP and investigation how differential expression CRNDE(P) influences metabolism chemoresistance ovarian cancer (OvCa) cells. We also assessed cellular localization changes CRNDEP,...
Gastrointestinal stromal tumours (GISTs) represent a heterogeneous group of mesenchymal origin characterized by gain-of-function mutations in KIT or PDGFRA the type III receptor tyrosine kinase family. Although either are thought to drive an early oncogenic event through similar pathways, two previous studies reported mutation-specific gene expression profiles. However, their further conclusions were rather discordant. To clarify molecular characteristics differentially expressed genes...
Although mitochondrial dysfunction is implicated in the pathogenesis of obesity, molecular mechanisms underlying obesity-related metabolic abnormalities are not well established. We performed quantitative proteomic and whole transcriptome analysis followed by functional annotations within liver skeletal muscles, using fasted non-fasted 16- 48-week-old high-fat diet (HFD)-fed normal diet-fed (control group) wild-type C56BL/6J mice, hyperphagic ob/ob db/db obese mice. Our study identified...
Protein immunoprecipitation (IP) coupled with MS provides means to interrogate protein complexes and their posttranslational modifications (PTMs). In a typical IP assay antibodies are conjugated A/G beads requiring large amounts of antibodies, tube transfers centrifugations.As an alternative, we present Matrix-IP, beads-free microplate-based platform surface-immobilized antibodies. Assay utilizes standard 96-well polypropylene PCR plates that laboratory-fabricated UV-C light then coated...
HAX1 protein is involved in the regulation of apoptosis, cell motility and calcium homeostasis. Its overexpression was reported several tumors, including breast cancer. This study demonstrates that has an impact on collective, but not single-cell migration, thus indicating importance cell-cell contacts for HAX1-mediated effect. Accordingly, it shown knockdown affects junctions, substrate adhesion, epithelial layer integrity. As demonstrated here, these effects can be attributed to modulation...
Abstract HCLS1-associated protein X-1 (HAX1) is a multifunctional involved in many cellular processes, including apoptosis, cell migration and calcium homeostasis, but its mode of action still remains obscure. Multiple HAX1 partners have been identified, they are distinct pathways, form different complexes do not constitute coherent group. By characterizing interactome using targeted approach, we attempt to explain multiple functions role the cell. Presented analyses indicate that interacts...
Although the degradome, which comprises proteolytic fragments of blood proteins, presents a potential source diagnostic biomarkers, studies on cancer peptide biomarkers have provided inconsistent conclusions. In present study, we reevaluated usefulness serum degradome analyses for searching biomarker candidates. Particular attention was paid to pre-analytical factors influencing variability determined levels, including clotting time and control group selection. Studies were conducted 44 86...