A5060343319- Redox biology and oxidative stress
- Histone Deacetylase Inhibitors Research
- MicroRNA in disease regulation
- Protein Degradation and Inhibitors
- Retinoids in leukemia and cellular processes
- BRCA gene mutations in cancer
- Plant tissue culture and regeneration
- Immune cells in cancer
- Genomic variations and chromosomal abnormalities
- Multiple Myeloma Research and Treatments
- Acute Myeloid Leukemia Research
- Helicobacter pylori-related gastroenterology studies
- RNA Research and Splicing
- Genetic Associations and Epidemiology
- Circular RNAs in diseases
- Esophageal Cancer Research and Treatment
- RNA modifications and cancer
- Glutathione Transferases and Polymorphisms
- Mitochondrial Function and Pathology
- Genetic factors in colorectal cancer
- Genomics and Chromatin Dynamics
- Chronic Lymphocytic Leukemia Research
- DNA Repair Mechanisms
- TGF-β signaling in diseases
- Hematological disorders and diagnostics
University of Warsaw
2016-2019
Medical University of Warsaw
2013-2018
Cancer Genetics (United States)
2018
Postgraduate School of Molecular Medicine
2010-2014
The Maria Sklodowska-Curie National Research Institute of Oncology
2007-2014
Warsaw University of Life Sciences
2004-2005
Tumor-driven immune suppression is a major barrier to successful immunotherapy in ovarian carcinomas (OvCa). Among various mechanisms responsible for suppression, arginase-1 (ARG1)-carrying small extracellular vesicles (EVs) emerge as important contributors tumor growth and escape from the host system. Here, we report that EVs found ascites plasma of OvCa patients contain ARG1. suppress proliferation CD4+ CD8+ T-cells vitro vivo mouse models. In mice, ARG1-containing are transported draining...
BackgroundAberrant DNA methylation in gene promoters is associated with aging and cancer, but the circumstances determining change are unknown. We investigated impact of lifestyle modulators colorectal cancer (CRC) risk on stability promoter colonic mucosa.
Peroxiredoxin-1 (PRDX1) is a multifunctional protein, acting as hydrogen peroxide (H2O2) scavenger, molecular chaperone and immune modulator. Although differential PRDX1 expression has been described in many tumors, the potential role of breast cancer remains highly ambiguous. Using comprehensive antibody-based proteomics approach, we interrogated protein putative biomarker estrogen receptor (ER)-positive cancer. An anti-PRDX1 antibody was validated cell lines using immunoblotting,...
The single nucleotide polymorphism (SNP) rs2542151 within the gene locus region encoding protein tyrosine phosphatase non-receptor type 2 (PTPN2) has been associated with Crohn's disease (CD), ulcerative colitis (UC), type-I diabetes, and rheumatoid arthritis. We have previously shown that PTPN2 regulates mitogen-activated kinase (MAPK) signaling cytokine secretion in human THP-1 monocytes intestinal epithelial cells (IEC). Here, we studied whether intronic SNP rs1893217 immune responses to...
Our previous work has shown peroxiredoxin-1 (PRDX1), one of major antioxidant enzymes, to be a biomarker in human breast cancer. Hereby, we further investigate the role PRDX1, compared its close homolog PRDX2, mammary malignant cells. CRISPR/Cas9- or RNAi-based methods were used for genetic targeting PRDX1/2. Cell growth was assessed by crystal violet, EdU incorporation colony formation assays. In vivo xenotransplantation model. Adenanthin inhibit thioredoxin-dependent defense system. The...
Pathogenesis of inflammatory bowel diseases (IBD), ulcerative colitis (UC) and Crohn's disease (CD), involves interaction between environmental factors inappropriate immune responses in the intestine genetically predisposed individuals. Bile acids their nuclear receptor, FXR, regulate barrier function intestinal tract.We studied association five variants (rs3863377, rs7138843, rs56163822, rs35724, rs10860603) NR1H4 gene encoding FXR with IBD. 1138 individuals (591 non-IBD, 203 UC, 344 CD)...
// Anna Trzeciecka 1 , Szymon Klossowski 2 Malgorzata Bajor Radoslaw Zagozdzon 1, 3 Pawel Gaj Angelika Muchowicz Agata Malinowska 4 Czerwoniec 5 Joanna Barankiewicz 6 Antoni Domagala Justyna Chlebowska 7 Monika Prochorec-Sobieszek 8, 9 Magdalena Winiarska Ryszard Ostaszewski Iwonna Gwizdalska 10 Jakub Golab Dominika Nowis 7, 11 Firczuk Department of Immunology, Medical University Warsaw, Poland Institute Organic Chemistry, Polish Academy Sciences, Bioinformatics, Biochemistry and Biophysics,...
Bezafibrate (BZ) regulates mitochondrial biogenesis by activation of PPAR's receptors and enhancing the level PGC-1α coactivator. In this report, we investigated effect BZ on expression genes (1) that are linked to different pathways involved in biogenesis, e.g., regulated or coactivator, (2) neuronal astroglial fate, during neural differentiation hiPSC. The tested cell populations included hiPSC-derived stem cells (NSC), early progenitors (eNP), (NP). RNA-seq analysis showed PPARA, PPARD...
Definitive identification of promoters, their cis-regulatory motifs, and trans-acting proteins requires experimental analysis. To define the HNRNPK promoter its cognate DNA–protein interactions, we performed a comprehensive study combining approaches, including luciferase reporter gene assays, chromatin immunoprecipitations (ChIP), electrophoretic mobility shift assays (EMSA), mass spectrometry (MS). We discovered that out four potential promoters tested, one containing palindromic motif...
Background Prostate cancer (PCa) and colorectal (CRC) are the most commonly diagnosed cancers cancer-related causes of death in Poland. To date, numerous single nucleotide polymorphisms (SNPs) associated with susceptibility to both types have been identified, but their effect on disease risk may differ among populations. Methods identify new SNPs PCa CRC Polish population, a genome-wide association study (GWAS) was performed using DNA sample pools Affymetrix Genome-Wide Human SNP 6.0 arrays....
Abstract Background Numerous papers have addressed the association of mutations and polymorphisms susceptibility genes with autoimmune inflammatory disorders. We investigated whether that confer to Crohn's disease could be classified also as predisposing factors for development primary sclerosing cholangitis biliary cirrhosis in Polish patients. Methods The study included 60 patients CD, 77 PSC, which 61 exhibited IBD (40 UC, 8 13 indeterminate colitis), 144 PBC. All were screened against...
Defined changes in the cell wall directed by many proteins accompany every morphogenetic process plants. Xyloglucan endotransglucosylase/hydrolase (XTH; EC 2.4.1.207) have potential to modify hemicellulose matrix within wall. Cs-XTH1 and Cs-XTH3 genes, which encode XTH proteins, were found among numerous genes that are differentially expressed after induction of cucumber somatic embryogenesis. The expression these increased during gene was localized on second chromosome near centromere...
Gene promoters contain several classes of functional sequence elements (cis elements) recognized by protein agents, e.g. transcription factors and essential components the machinery. Here we describe a common DNA regulatory element (tandem TCTCGCGAGA motif) human TATA-less promoters. A combination bioinformatic experimental methodology suggests that can be critical for expression genes involved in enhanced synthesis G1/S transition cell cycle. The motif was identified substantial fraction...
Late onset Alzheimer's disease (LOAD) accounts for about 95% of all cases. While the APOE ε4 variant seems to have unparalleled influence on increased LOAD risk, it does not explain heritability LOAD. In this study,
circRNAs constitute a novel class of RNA, generally considered as non-coding RNAs; nonetheless, their coding potential has been under scrutiny. In this work, we systematically explored the predicted proteins more than 160,000 detected by exome capture RNA-sequencing and collected in MiOncoCirc pan-cancer compendium, including normal cancer samples from different types tissues. For functional evaluation, compared primary structure domain composition with those derived same linear mRNAs. Among...
Objectives: Peroxiredoxins (PRDXs) constitute a family of antioxidant enzymes which are also involved in the process carcinogenesis. They composed six identified isoforms (PRDX-1-6) and supposed to play different roles tumor progression, depending on type cancer member PRDX family. The aim study was assess prog- nostic value PRDXs ovarian cancer. Material methods: dataset patients with from Cancer Genome Atlas analyzed. Expression PRDX-1 6 mRNA evaluated 260 samples. prognostic assessed...
MicroRNAs, non-coding regulators of gene expression, are known culprits thyroid cancer. Using next-generation sequencing, we identified a novel microRNA gene, encoded within an important regulator - thyroglobulin, and analyzed its functionality in the gland. In vitro silico analyses proved that miR-TG is processed from precursor, co-expressed with thyroglobulin. Both genes specific for tissue downregulated papillary carcinoma by 44% (p = 0.04) 48% 0.001), respectively. Putative target were...
Colorectal cancer (CRC) is an epidemiological problem of a great importance in Poland; each year approximately 14,600 new cases the disease are diagnosed. Mortality associated with CRC reaches 10,400 per (according to National Cancer Registry). The 5-year survival rate 25 %, which one lowest rates Europe. etiology sporadic colorectal multifactorial and has been attributed interplay between both environmental genetic risk factors. In addition, there general consensus that factors may modulate...
Cereblon (CRBN) is crucial for antiproliferative and immunomodulatory properties of drugs. The objective this study was to verify whether germline single nucleotide polymorphisms (SNPs) in the CRBN gene may influence response lenalidomide multiple myeloma (MM). Fourteen tagging SNPs covering genetic variability region were genotyped 167 Polish patients with refractory/relapsed MM treated lenalidomide-based regimens. We found that carriers minor alleles two studied rs1714327G > C (OR = 0.26;...
A cute myeloid leukemia is a malignant disease of immature cells. Despite significant therapeutic effects differentiation-inducing agents in some acute subtypes, the remains incurable large fraction patients. Here we show that SK053, thioredoxin inhibitor, induces differentiation and cell death Considering knock-down with short hairpin RNA failed to exert antiproliferative one lines, used biotin affinity probe-labeling approach identify potential molecular targets for SK053. Mass...