A5033334712- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
- Computational Drug Discovery Methods
- Protein Structure and Dynamics
- Influenza Virus Research Studies
- RNA modifications and cancer
- Immune Cell Function and Interaction
- RNA Research and Splicing
- Retinoids in leukemia and cellular processes
- Mitochondrial Function and Pathology
- CAR-T cell therapy research
- RNA regulation and disease
- Protein Degradation and Inhibitors
- Virus-based gene therapy research
- Melanoma and MAPK Pathways
- Microbial Natural Products and Biosynthesis
- Erythrocyte Function and Pathophysiology
- Viral gastroenteritis research and epidemiology
- Phagocytosis and Immune Regulation
- Cell Adhesion Molecules Research
- Hypertrophic osteoarthropathy and related conditions
- NMR spectroscopy and applications
- Endoplasmic Reticulum Stress and Disease
- Viral Infections and Vectors
- Epigenetics and DNA Methylation
University of Warsaw
2012-2024
Warsaw University of Technology
2019-2023
National Institute of Public Health
2023
Medical University of Warsaw
2010-2019
Gdańsk Medical University
2019
Postgraduate School of Molecular Medicine
2019
National Cancer Institute
2019
Genomics (United Kingdom)
2018
Abstract Docking is one of the most commonly used techniques in drug design. It for both identifying correct poses a ligand binding site protein as well estimation strength protein–ligand interaction. Because millions compounds must be screened, before suitable target biological testing can identified, all calculations should done reasonable time frame. Thus, programs currently use exploit empirically based algorithms, avoiding systematic search conformational space. Similarly, scoring using...
Squalene epoxidase (SE) is a key flavin adenine dinucleotide (FAD)-dependent enzyme of ergosterol and cholesterol biosynthetic pathways an attractive potential target for drugs used to inhibit the growth pathogenic fungi or lower level. Although many studies on allylamine activity have been published during last 30 years, up until now no detailed mechanism squalene inhibition has presented. Our study brings such model at atomic resolution in case yeast Saccharomyces cerevisiae. Presented...
Molecular recognition plays a fundamental role in all biological processes, and that is why great efforts have been made to understand predict protein-ligand interactions. Finding molecule can potentially bind target protein particularly essential drug discovery still remains an expensive time-consuming task. In silico, tools are frequently used screen molecular libraries identify new lead compounds, if structure known, various docking programs be used. The aim of procedure correct poses...
C16orf57 encodes a human protein of unknown function, and mutations in the gene occur poikiloderma with neutropenia (PN), which is rare, autosomal recessive disease. Interestingly, were also observed among patients diagnosed Rothmund-Thomson syndrome (RTS) dyskeratosis congenita (DC), are caused by genes involved DNA repair telomere maintenance. A genetic screen Saccharomyces cerevisiae revealed that yeast ortholog , USB1 ( YLR132C ), essential for U6 small nuclear RNA (snRNA) biogenesis...
Significance Cellular function is tightly regulated by large molecular assemblies such as the proteasome, which involved in removal of damaged or misfolded proteins. Proteasome substrates are unfolded complexes valosin-containing protein-like ATPase Thermoplasma acidophilum (VAT) via a process that coupled to ATP hydrolysis. We used combined electron cryomicroscopy (cryo-EM) and NMR analysis show VAT undergoes large, previously unidentified, conformational changes essential for substrate...
For the last two years, COVID-19 pandemic has continued to bring consternation on most of world. According recent WHO estimates, there have been more than 5.6 million deaths worldwide. The virus continues evolve all over world, thus requiring both vigilance and necessity find develop a variety therapeutic treatments, including identification specific antiviral drugs. Multiple studies confirmed that SARS-CoV-2 utilizes its membrane-bound spike protein recognize human angiotensin-converting...
Hearing loss and ovarian dysfunction are key features of Perrault syndrome (PRLTS) but the clinical pathophysiological hearing impairment in PRLTS individuals have not been addressed. Mutations one five different genes HSD17B4, HARS2, LARS2, CLPP or TWNK (previous symbol C10orf2) cause autosomal recessive disorder they found only about half patients.We report on two siblings with a picture resembling severe, neurological type PRLTS. For an exhaustive characterisation phenotype neuroimaging...
Abstract Oxidative stress, caused by the imbalance between reactive species generation and dysfunctional capacity of antioxidant defenses, is one characteristic features cancer. Here, we quantified hydrogen peroxide in tumor microenvironment (TME) demonstrated that concentrations are elevated interstitial fluid isolated from murine breast cancers vivo, when compared with blood or normal subcutaneous fluid. Therefore, investigated effects increased concentration on immune cell functions. NK...
CD71
•Individuals with macro-AST demonstrate fluctuating, not persistent, elevation of AST levels in blood.•The causative gene has yet been identified.•A genetic variant associated was found theGOT1 gene.•Testing for this could aid diagnosis macro-AST. Background & AimsMacro-aspartate aminotransferase (macro-AST) manifests as a persistent levels, because association the protein immunoglobulins circulation. Macro-AST is rare, benign condition without previously confirmed basis.MethodsWhole exome...
Metastasis to distant organs is a major cause for solid cancer mortality, and the acquisition of migratory invasive phenotype key factor in initiation malignancy. In this study we investigated contribution Mixed-Lineage Kinase 4 (MLK4) aggressive breast cells. Our TCGA genomic data analysis revealed that amplification or mRNA upregulation MLK4 occurred 23% carcinoma cases. To find association between expression specific subtype cancer, performed transcriptomic multiple datasets, which showed...
Processing of exogenous glycerol esters is an initial step in energy derivation for many bacterial cells. Lipid-rich environments settled by a variety organisms exert strong evolutionary pressure establishing enzymatic pathways involved lipid metabolism. However, certain number enzymes this process remain unknown since they do not share detectable sequence similarity with any known protein domains. Using distant homology detection and fold recognition we predict that transmembrane proteins...
Malignant mesothelioma is a rare and aggressive form of cancer. Despite improvements in cancer treatment, there are still no curative treatment modalities for advanced stage the malignancy. The aim this study was to evaluate anti-tumor efficacy novel combinatorial therapy combining AdV5/3-D24-ICOSL-CD40L, an oncolytic vector, with anti-PD-1 monoclonal antibody. vector confirmed vitro three cell lines - H226, Mero-82, MSTO-211H, subsequently antineoplastic properties combination evaluated...
Abstract Intermolecular interactions of protein–protein complexes play a principal role in the process discovering new substances used diagnosis and treatment many diseases. Among such proteins, we have to mention antibodies; they interact with specific antigens two genera single-stranded RNA viruses belonging family Filoviridae—Ebolavirus Marburgvirus; both cause rare but fatal viral hemorrhagic fever Africa, pandemic potential. In this research, conduct studies aimed at design evaluation...
The Cyclin-Dependent Kinases (CDKs) are the core components coordinating eukaryotic cell division cycle. Generally crystal structure of CDKs provides information on possible molecular mechanisms ligand binding. However, reliable and robust estimation binding activity has been a challenging task in drug design. In this regard, various machine learning techniques, such as Support Vector Machine, Naive Bayesian classifier, Decision Tree, K-Nearest Neighbor have used. performance these...
The influenza virus type A (IVA) is an important pathogen which able to cause annual epidemics and even pandemics. This fact the consequence of antigenic shifts drifts capabilities IVA, caused by high mutation rate reassortment virus. hemagglutinin (HA) protein constitutes main IVA antigen has a crucial role in infection mechanism, being responsible for recognition host-specific sialic acid derivatives. Despite relative abundance HA sequence serological studies, comparative structure-based...
Several TBC1D24 variants are causally involved in the development of profound, prelingual hearing loss (HL) and different epilepsy syndromes inherited an autosomal recessive manner. Only two pathogenic have been linked with postlingual progressive dominant HL (ADHL). To determine role ADHL to characterize TBC1D24-related ADHL, clinical exome sequencing or targeted multigene (n = 237) panel were performed for probands 102) from multigenerational families. In four families, was found based on...
A cute myeloid leukemia is a malignant disease of immature cells. Despite significant therapeutic effects differentiation-inducing agents in some acute subtypes, the remains incurable large fraction patients. Here we show that SK053, thioredoxin inhibitor, induces differentiation and cell death Considering knock-down with short hairpin RNA failed to exert antiproliferative one lines, used biotin affinity probe-labeling approach identify potential molecular targets for SK053. Mass...