A5101782163- Osteoarthritis Treatment and Mechanisms
- Knee injuries and reconstruction techniques
- Total Knee Arthroplasty Outcomes
- Periodontal Regeneration and Treatments
- Mesenchymal stem cell research
- Bone Tissue Engineering Materials
- Nanoplatforms for cancer theranostics
- Immune Cell Function and Interaction
- Inflammatory mediators and NSAID effects
- Dendrimers and Hyperbranched Polymers
- RNA Interference and Gene Delivery
- Nanoparticle-Based Drug Delivery
- Immune cells in cancer
- Collagen: Extraction and Characterization
- T-cell and B-cell Immunology
- Tissue Engineering and Regenerative Medicine
- CAR-T cell therapy research
- Bone and Joint Diseases
- Shoulder Injury and Treatment
- Electrospun Nanofibers in Biomedical Applications
- Silk-based biomaterials and applications
- Proteoglycans and glycosaminoglycans research
- Advanced Nanomaterials in Catalysis
- Lower Extremity Biomechanics and Pathologies
- Angiogenesis and VEGF in Cancer
University of Florida
2015-2024
UF Health Cancer Center
2024
University of Florida Health
2024
Florida College
2021
University of Arkansas at Little Rock
2018
Cleveland Clinic Lerner College of Medicine
2010-2016
Johns Hopkins University
2003-2013
Cleveland Clinic
2011
Surgical options for cartilage resurfacing may be significantly improved by advances and application of biomaterials that direct tissue repair. A poly(ethylene glycol) diacrylate (PEGDA) hydrogel was designed to support matrix production, with easy surgical application. model in vitro system demonstrated deposition cartilage-specific extracellular the biomaterial stimulation adjacent development mesenchymal stem cells. For translation joint environment, a chondroitin sulfate adhesive applied...
Cartilage tissue engineering strategies generally result in homogeneous structures with little resemblance to the native zonal organization of articular cartilage. The objective this study was use bilayered photopolymerized hydrogels organize zone-specific chondrocytes a stratified framework and effects three-dimensional coculture system on properties engineered tissue. Superficial deep zone from bovine cartilage were photoencapsulated separate as well adjacent layers hydrogel. Histology,...
A major hurdle limiting the ability to treat and cure osteoarthritis, a common debilitating disease, is rapid joint clearance limited cartilage targeting of intra-articular therapies. Nanoscale drug carriers have potential improve therapeutic retention in after direct injection; however, there still lacks fundamental understanding how physicochemical properties nanoparticles (NPs) influence localization degenerating conditions such as disease state synovial fluid impact NP biodistribution....
We investigated the biological response of chondrocytes isolated from different zones articular cartilage and their cellular behaviors in poly (ethylene glycol)‐based (PEG) hydrogels containing exogenous type I collagen, hyaluronic acid (HA), or chondroitin sulfate (CS). The morphology was strongly dependent on extracellular matrix component hydrogels. Additionally, microenvironment affected production specific gene expression zones. CS‐based showed strongest terms accumulation for both...
Abstract Oxidative stress, caused by the imbalance between reactive species generation and dysfunctional capacity of antioxidant defenses, is one characteristic features cancer. Here, we quantified hydrogen peroxide in tumor microenvironment (TME) demonstrated that concentrations are elevated interstitial fluid isolated from murine breast cancers vivo, when compared with blood or normal subcutaneous fluid. Therefore, investigated effects increased concentration on immune cell functions. NK...
Objective To determine performance and repair kinetics of the ChonDux hydrogel scaffold for treating focal articular cartilage defects in knee over 24 months. Design This assessor-blinded trial evaluates implantation combination with microfracture 18 patients across 6 sites. Male female to 65 years age full-thickness femoral condyle 2 4 cm area were enrolled. Eligible received treatment followed by rehabilitation. Defect volume fill was evaluated after 3, (primary outcome), 12, 18, months...
Natural killer (NK) cells, which are an exciting alternative cell source for cancer immunotherapies, must sense and respond to their physical environment traffic eliminate cells. Herein, we review the mechanisms by NK cells receive mechanical signals explore recent key findings regarding impact of characteristics solid tumors on functions. Data suggest that different stresses present in facilitate functions, especially infiltration degranulation. Moreover, engineering advances can be used...
Despite numerous efforts in cartilage regeneration, few products see the light of clinical translation as commercialization process is opaque, financially demanding, and requires collaboration with people varied skill sets. The aim this review to introduce, an academic audience, different paradigms involved regeneration technology, elucidate hurdles associated use cells materials developing new technologies, discuss potential strategies, inform reader about current trends observed both...
ABSTRACT Small animal models are essential for studying anterior cruciate ligament (ACL) injury, one of the leading risk factors post‐traumatic osteoarthritis (PTOA). Non‐surgical ACL rupture have recently surged as a new tool to study PTOA, they circumvent confounding effects surgical disruption joint. These primarily been explored in mice and rabbits, but relatively understudied rats. The purpose this work was establish non‐invasive, mechanical overload model rat disease pathogenesis...
The production of engineered cartilage from mesenchymal stem cells is a rapidly developing field. Potential applications include the treatment degenerative joint disease as well traumatic and surgical bone injury. Prior to clinical application, however, further characterization morphology, ultrastructure, biocompatibility, performance tissue warranted. To achieve this, human (hMSCs) were grown in vitro pellet culture for 3 weeks chondrogenic medium conditions. resultant was compared native...
A critical hurdle associated with natural killer (NK) cell immunotherapies is inadequate infiltration and function in the solid tumor microenvironment. Well-controlled 3D culture systems could advance our understanding of role various biophysical biochemical cues that impact NK migration tumors. The objectives this study were to establish a biomaterial which (i) supports (ii) recapitulates features vivo microenvironment, system. Using peptide-functionalized poly(ethylene glycol)-based...