A5033410736- Mesenchymal stem cell research
- Periodontal Regeneration and Treatments
- Osteoarthritis Treatment and Mechanisms
- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- dental development and anomalies
- Bone Tissue Engineering Materials
- Craniofacial Disorders and Treatments
- Endodontics and Root Canal Treatments
- Bone fractures and treatments
- 3D Printing in Biomedical Research
- Cleft Lip and Palate Research
- Dental Implant Techniques and Outcomes
- Dental Trauma and Treatments
- Biomedical Ethics and Regulation
- Knee injuries and reconstruction techniques
- Tendon Structure and Treatment
- Bone and Dental Protein Studies
- Bone Metabolism and Diseases
- Proteoglycans and glycosaminoglycans research
- Orthodontics and Dentofacial Orthopedics
- Cellular Mechanics and Interactions
- Orthopaedic implants and arthroplasty
- Pluripotent Stem Cells Research
- Graphene and Nanomaterials Applications
Columbia University
2013-2023
Jiangnan University
2023
Columbia University Irving Medical Center
2011-2020
Mayo Clinic in Arizona
2018
Stomatology Hospital
2015-2018
Hospital of Stomatology, Sun Yat-sen University
2016-2018
Sun Yat-sen University
2015-2018
Cleveland Clinic
2018
Florida Craniofacial Institute
2015
New York University
2010
Tooth infections or injuries involving dental pulp are treated routinely by root canal therapy. Endodontically teeth devitalized, susceptible to re-infections, fractures, and subsequent tooth loss. Here, we report regeneration of dental-pulp-like tissue cell homing without transplantation. Upon in vivo implantation endodontically real-size, native human mouse dorsum for the tested 3 weeks, delivery basic fibroblast growth factor and/or vascular endothelial (bFGF VEGF) yielded re-cellularized...
Sheep knee meniscus was regenerated by spatiotemporal delivery of two recombinant human proteins in 3D-printed bioresorbable scaffold.
Aim: To determine whether fluid flow-induced shear stress affects the differentiation of bone marrow-derived human mesenchymal stem cells (hMSCs) into osteogenic cells. Materials & methods: hMSCs cultured with or without medium were exposed to and analyzed for alkaline phosphatase activity expression genes. Results: Immediately following stress, in was significantly increased. At days 4 8 culture mRNA morphogenetic protein-2 osteopontin higher subjected than those static conditions. However,...
Fibroblasts are ubiquitous cells that demonstrate remarkable diversity. However, their origin and pathways of differentiation remain poorly defined. Here, we show connective tissue growth factor (CTGF; also known as CCN2) is sufficient to induce human bone marrow mesenchymal stem/stromal (MSCs) differentiate into fibroblasts. CTGF-stimulated MSCs lost surface epitopes, expressed broad fibroblastic hallmarks, increasingly synthesized collagen type I tenacin-C. After commitment, the ability...
A National Institutes of Health sponsored workshop "Bone Tissue Engineering and Regeneration: From Discovery to the Clinic" gathered thought leaders from medicine, science, industry determine state art in field define barriers translating new technologies novel therapies treat bone defects. engineering holds enormous promise improve human health through prevention disease restoration healthy tissue functions. Bone engineering, similar that for other tissues organs, requires integration...
Tooth-supporting periodontium forms a complex with multiple tissues, including cementum, periodontal ligament (PDL), and alveolar bone. In this study, we developed multiphase region-specific microscaffolds spatiotemporal delivery of bioactive cues for integrated regeneration. Polycarprolactione-hydroxylapatite (90:10 wt%) scaffolds were fabricated using three-dimensional printing seamlessly in three phases: 100-μm microchannels Phase A designed cementum/dentin interface, 600-μm B the PDL,...
Current stem cell-based strategies for tissue regeneration involve ex vivo manipulation of these cells to confer features the desired progenitor population. Recently, concept that endogenous stem/progenitor could be used regenerating tissues has emerged as a promising approach potentially overcomes obstacles related cell transplantation. Here we applied this strategy injured tendons in rat model. First, identified rare fraction tendon was positive known marker CD146 and exhibited clonogenic...
Tooth regeneration by cell delivery encounters translational hurdles. We hypothesized that anatomically correct teeth can regenerate in scaffolds without transplantation. Novel, shaped human molar and rat incisor were fabricated 3D bioprinting from a hybrid of poly-epsilon-caprolactone hydroxyapatite with 200-microm-diameter interconnecting microchannels. In each 22 rats, an scaffold was implanted orthotopically following mandibular extraction, whereas ectopically into the dorsum....
Mesenchymal stem cells (MSCs) are progenitors for tissues such as bone and cartilage. In this report, the actin cytoskeleton nanomechanobiology of human mesenchymal (hMSCs) were studied using fluorescence microscopy atomic force (AFM). Human MSCs differentiated into chondrocytes osteoblasts per previous approaches. Cytochalasin D (CytD) was used to temporarily disrupt in hMSCs, hMSC-chondrocytes (hMSC-Cys) hMSC-osteoblasts (hMSC-Obs). Fluorescence revealed a dose-dependent response CytD....
The temporomandibular joint is susceptible to diseases and trauma that may ultimately lead structural degeneration. Current approaches for replacing degenerated mandibular condyles suffer from deficiencies such as donor site morbidity, immunorejection, implant wear tear, pathogen transmission. hypothesis of this study was a human-shaped condyle can be tissue-engineered rat mesenchymal stem cells (MSCs) encapsulated in biocompatible polymer. Rat bone marrow MSCs were isolated induced...
Soft tissue augmentation is a widespread practice in plastic and reconstructive surgery. The objective of the present study was to engineer adipose constructs with predefined shape dimensions, potentially utilizable soft reconstruction, by encapsulating adult stem cell-derived adipogenic cells biocompatible hydrogel system. Bone marrow-derived human mesenchymal (hMSCs) were preconditioned 1 week exposure adipogenic- inducing supplement followed photoencapsulation poly(ethylene glycol)...
Background: An entire articular condyle engineered from stem cells may provide an alternative therapeutic approach to total joint replacement. This study describes our continuing effort optimize the chondrogenic and osteogenic differentiation mesenchymal toward engineering condyles in vivo. Methods: Primary rat bone-marrow were induced differentiate into lineages vitro suspended polyethylene glycol-based hydrogel. The hydrogel cell suspensions, each at a density of 20 × 106 cells/mL,...
Dental pulp has intrinsic capacity for self-repair. However, it is not clear whether dental cells can be recruited endogenously regenerating tissues, including mineralizing into dentin. This work based on a hypothesis that stem/progenitor induced to migrate by chemotactic cytokines and act as endogenous cell sources regeneration mineralization. stem (DSCs) were isolated from adult human tooth seeded the surfaces of 3D collagen gel cylinders incubated in chemically defined media with...