A5039652422- Antimicrobial agents and applications
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Muscle activation and electromyography studies
- Bacterial biofilms and quorum sensing
- Bacterial Genetics and Biotechnology
- Microbial Metabolic Engineering and Bioproduction
- Neuroscience and Neural Engineering
- Fermentation and Sensory Analysis
- Antimicrobial Peptides and Activities
- Biochemical and biochemical processes
- Microplastics and Plastic Pollution
- biodegradable polymer synthesis and properties
- Antibiotic Resistance in Bacteria
- Biopolymer Synthesis and Applications
- Plant biochemistry and biosynthesis
- Muscle Physiology and Disorders
- Pharmaceutical and Antibiotic Environmental Impacts
- Microbial Metabolism and Applications
- Protein Hydrolysis and Bioactive Peptides
- Bacteriophages and microbial interactions
- Biofuel production and bioconversion
- Synthesis of Tetrazole Derivatives
- Enzyme Catalysis and Immobilization
- Analytical Chemistry and Chromatography
Warsaw University of Technology
2015-2024
University of Warsaw
2021
Czech Academy of Sciences, Institute of Biotechnology
2017
Institute of Biochemistry and Biophysics, Polish Academy of Sciences
2004-2012
Polish Academy of Sciences
1999-2008
Institute of Biocybernetics and Biomedical Engineering
1999-2006
Cationic polymers have been extensively investigated as a potential replacement for traditional antibiotics. Here, we examined the effect of molecular weight (MW) on antimicrobial, cytotoxic, and hemolytic activity linear polytrimethylenimine (L-PTMI). The results indicate that biological polymer sharply increases MW increases. Thanks to different position antibacterial toxicity thresholds, tuning PTMI allows one achieve therapeutic window between antimicrobial concentrations. L-PTMI...
ABSTRACT The par genes of Pseudomonas aeruginosa have been studied to increase the understanding their mechanism action and role in bacterial cell. Key properties ParB protein identified are associated with different parts protein. ParB- interaction domain was mapped vivo vitro C-terminal 56 amino acids (aa); 7 aa at C terminus play an important role. dimerization P. is interchangeable KorB from plasmid RK2 (IncP1 group). part also involved ParB-ParA interactions. Purified binds specifically...
In this study, we isolated 28 yeast strains from samples of plant material and fermented food evaluated the possibility efficient production 2‐phenylethanol (2‐PE) in organic waste‐based media supplemented with l‐phenylalanine (l‐Phe). We used whey, a by‐product milk processing, as base for media, either glucose or three by‐products sugar beet processing fermentable carbon source. Ten newly were capable producing over 2 g l−1 2‐PE through l‐Phe biotransformation batch mode standard medium....
The parA and parB genes of Pseudomonas aeruginosa are located approximately 8 kb anticlockwise from oriC. ParA is a cytosolic protein present at level around 600 molecules per cell in exponential phase, but the drops about fivefold stationary phase. Overproduction full-length or N-terminal 85 amino acids severely inhibits growth P. putida. Both inactivation overexpression trans also lead to accumulation anucleate cells changes motility. Inactivation increases turnover rate (degradation)...
Motor unit (MU) potentials were recorded from brachial biceps of healthy subjects aged 5.5-79 years. The subdivided into young (5.5-19 year) and adult (37.5-79 groups, between which single MU discharge characteristics compared. Firing rates in the ranges 8.3-21.7 s(-1) (mean 12.87 s(-1)) 5.9-18.7 11.08 for respectively. Standard deviations (s.d.) interspike intervals (ISIs) range 4.84-11.57 ms 8.39 ms) group 4.26-12.23 7.76 group. Both differences statistically significant (P < 0.001)....
Deletions leading to complete or partial removal of ParB were introduced into the Pseudomonas aeruginosa chromosome. Fluorescence microscopy fixed cells showed that mutants lacking C-terminal domain HTH motif formed multiple, less intense foci scattered irregularly, in contrast one four per cell symmetrically distributed wild-type P. aeruginosa. All parB mutations affected both bacterial growth and swarming swimming motilities, increased production anucleate cells. Similar effects observed...
Extracellular vesicles (EVs) are nanoparticles containing various bioactive cargos-e.g., proteins, RNAs, and lipids-that released into the environment by all cell types. They involved in, amongst other functions, intercellular communication. This article presents studies on EVs produced probiotic yeast Saccharomyces boulardii CNCM I-745. The size distribution concentration of in liquid culture were estimated. Moreover, S. tested for their cytotoxicity against three model human intestinal...
Sixteen cold-adapted reddish-pigmented yeast strains were obtained from environmental samples. According to the PCR-based detection of classical markers combined with phylogenetic studies, yeasts belong mainly genera Rhodotorula, Sporobolomyces and Cystobasidium, all within subphylum Pucciniomycotina. All produced carotenoids a 0.25-10.33 mg/L range under non-optimized conditions. Noteworthily, among them, representatives Cystobasidium genus found; particular value are C. laryngis...
Incorporation of hydrophobic component into amphiphilic polycations structure is frequently accompanied by an increase antimicrobial activity. There is, however, a group relatively hydrophilic containing quaternary ammonium moieties along mainchain, ionenes, which also display strong and limited hemolytic properties. In this work, influence side length on mechanism action investigated in series novel ionenes. High activity was found determination minimum inhibitory concentration (MIC)...
Abstract BACKGROUND Plant biomass rich in lignocelluloses may be applicable for the production of highly valuable bioproducts. In this study, use hydrolyzed corn stover as a carbon source yeast producing 2‐phenylethanol (2‐PE) is investigated. 2‐PE an alcohol with rosy scent, commonly used food and cosmetic industries aroma preservative. The growing demand compound motivates development new methods production. RESULTS was first pretreated alkaline solution H 2 O secondly hydrolysed...
Abstract The antimicrobial properties of polycations are strongly affected by the structural features such as backbone flexibility and topology (isomerism) through polymer ability to attain proper conformation in interaction with cell membrane. In this paper, a synthesis biocidal evaluation ionenes characterized different presented. findings reveal influence variation on activity against microorganisms, general positive effect improved flexibility. Furthermore, one obtained displays...
2-Phenylethanol (2-PE) is an aromatic alcohol with a rosy scent which widely used in the food, fragrance, and cosmetic industries. Promising sources of natural 2-PE are microorganisms, especially yeasts, can produce by biosynthesis biotransformation. Thus, first challenging goal development biotechnological production searching for highly productive yeast strains. In present work, 5 laboratory Saccharomyces cerevisiae strains were tested 2-PE. Thereafter, 2 them hybridized mating procedure...
Abstract Polycations, mimicking activity of antibacterial peptides, belong to an important class molecules investigated as a support or alternative antibiotics. In this work, studies modified linear amphiphilic statistical polymethyloxazoline (PMOX) and polyethyleneimine copolymers (PMOX_PEI) series are presented. Variation PEI content in the structure results controllable changes polymeric aggregates zeta potential. The with highest positive charge shows best antimicrobial activity, well...
The Maf1 protein is a general negative repressor of RNA polymerase III, which conserved in eukaryotes from yeast to humans. Herein, we show the maf1Δ mutant increases pyruvate kinase activity, key enzyme glycolysis and an important player switching between fermentative oxidative metabolism. We observed enhanced ethanol production elevated lipid content strain grown on glucose. However, after shifting non-fermentable carbon source, opposite effect was observed, cells accumulated smaller...
Abstract Double discharges of motor units (MUs) occurring during sustained voluntary muscle contractions are observed occasionally in healthy muscles and more frequently disorders the neuromuscular system. In subjects, double generated motoneurons (MNs) considered to be a sign their increased excitability. Therefore, an analysis firing pattern may provide information on state MNs diseases, particularly amyotrophic lateral sclerosis (ALS), whose etiology remains disclosed. Firing patterns MUs...
The ParB protein of Pseudomonas aeruginosa is important for growth, cell division, nucleoid segregation and different types motility. To further understand its function we have demonstrated a vital role the hydrophobic residues in C terminus ParB(P.a.). By silico modelling C-terminal domain (amino acids 242-290) L282, V285 I289 (but not L286) are engaged leucine-zipper-like structure formation, whereas charged R290 Q266 implicated forming salt bridge involved stabilization. Five parB mutant...