A5087824882- Telomeres, Telomerase, and Senescence
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Curcumin's Biomedical Applications
- RNA Interference and Gene Delivery
- DNA Repair Mechanisms
- Signaling Pathways in Disease
- Cancer-related Molecular Pathways
- Nitric Oxide and Endothelin Effects
- Extracellular vesicles in disease
- Retinoids in leukemia and cellular processes
- MicroRNA in disease regulation
- Microtubule and mitosis dynamics
- Neuroinflammation and Neurodegeneration Mechanisms
- Genomics, phytochemicals, and oxidative stress
- Protein Tyrosine Phosphatases
- Anesthesia and Neurotoxicity Research
- Circadian rhythm and melatonin
- Advanced biosensing and bioanalysis techniques
- Cell death mechanisms and regulation
- Chronic Lymphocytic Leukemia Research
- Gut microbiota and health
- Diet and metabolism studies
- Cancer therapeutics and mechanisms
- Cytomegalovirus and herpesvirus research
- Immunotherapy and Immune Responses
Polish Academy of Sciences
2015-2024
Instytut Biologii Doświadczalnej im. Marcelego Nenckiego
2015-2024
University College Cork
2010
University of Bern
1996
Cancer cells can undergo stress-induced premature senescence, which is considered to be a desirable outcome of anticancer treatment. However, the escape from senescence and cancer cell repopulation give rise some doubts concerning effectiveness senescence-induced therapy. Similarly, it postulated that polyploidization connected with disease relapse. We postulate associated culprit atypical divisions leading regrowth. Accordingly, we aimed dissociate between these two phenomena. induced in...
Senescence of vascular smooth muscle cells (VSMCs) contributes to aging as well age-related diseases the cardiovascular system. Senescent VSMCs have been shown be present in atherosclerotic plaques. Both replicative (RS) and stress-induced premature senescence (SIPS) accompany diseases. We aimed establish signature RS SIPS VSMCs, induced by a common anticancer drug, doxorubicin, discover so far undisclosed features senescent that are potentially harmful organism. Most hallmarks were for both...
Trimethylamine-N-oxide (TMAO) has been suggested as a marker and mediator of cardiovascular diseases. However, data are contradictory, the mechanisms obscure. Strikingly, role TMAO precursor trimethylamine (TMA) not drawn attention in studies even though toxic effects TMA were proposed several decades ago. We assessed plasma levels healthy humans (HH) patients qualified for aortic valve replacement (CP). The cytotoxicity rat cardiomyocytes was evaluated using an MTT test. on albumin lactate...
Aging | doi:10.18632/aging.203835. Adam Krzystyniak, Malgorzata Wesierska, Gregory Petrazzo, Agnieszka Gadecka, Magdalena Dudkowska, Anna Bielak-Zmijewska, Grazyna Mosieniak, Izabela Figiel, Jakub Wlodarczyk, Ewa Sikora
Atherosclerosis, a common age-related disease, is characterized by intense immunological activity. Atherosclerotic plaque composed of endothelial cells, vascular smooth muscle cells (VSMCs), lipids and immune infiltrating from the blood. During progression VSMCs undergo senescence within secrete SASP (senescence-associated secretory phenotype) factors that can actively modulate microenvironment. We demonstrated senescent increased number extracellular vesicles (senEVs). Based on unbiased...
Abstract The Bcr-Abl oncoprotein plays a major role in the development and progression of chronic myeloid leukemia is determinant chemotherapy resistance occurring during blast crisis phase disease. aim this article was to investigate possibility combating apoptosis caused by inducing an alternative cell death process. As model leukemia, we employed Bcr-Abl-transfected mouse progenitor 32D cells with low high expression levels corresponding drug-sensitive drug-resistant cells, respectively....
Tetraspanins, including CD9, CD63, and CD81, are transmembrane biomarkers that play a crucial role in regulating cancer cell proliferation, invasion, metastasis, as well plasma membrane dynamics protein trafficking. In this study, we developed simple, fast, sensitive immunosensors to determine the concentration of extracellular vesicles (EVs) isolated from human lung cells using tetraspanins biomarkers. We employed surface plasmon resonance (SPR) quartz crystal microbalance with dissipation...
Extracellular vesicles (EVs) released from primary cell lines, originating resected tissues during biopsies in patients with non-small lung cancer (NSCLC) revealing adenocarcinoma and squamous carcinoma subtypes, were examined for membrane proteomic fingerprints using a proximity barcoding assay. All the collected EVs expressed canonical tetraspanins (CD9, CD63, CD81) highly coexpressed molecules such as lysosome-associated protein-1 (LAMP1-CD107a), sialomucin core protein 24 (CD164), Raph...
Abstract Curcumin is a natural pigment that has been shown to induce cell death in many cancer cells; however, the mode depends on type and curcumin concentration. Here we show that, Jurkat cells, 50 μmol/L severely lowers survival induces initial stage of chromatin condensation. It also caspase-3, which sufficient cleave DNA fragmentation factor 45 [DFF45/inhibitor caspase-activated DNase (ICAD)], inhibitor DFF40/CAD endonuclease. However, release from its does not lead oligonucleosomal...
Senescence is a stress response characterized by an irreversible growth arrest and alterations in certain cell functions. It believed that both double-strand DNA breaks (DSB) increased ROS level are the main culprit of senescence. Excessive production also particularly important development number cardiovascular disorders. In this context involvement professional ROS-producing enzymes, NADPH oxidases (NOX), was postulated. contrary to common knowledge, we have shown not only but diminished...
Abstract It has been suggested that trimethylamine oxide (TMAO), a liver oxygenation product of gut bacteria-produced (TMA), is marker cardiovascular risk. However, mechanisms the increase and biological effects TMAO are obscure. Furthermore, potential role precursor, TMA, not investigated. We evaluated effect age, risk factor, on plasma levels TMA TMAO, bacteria composition, gut-to-blood penetration histological hemodynamic parameters in 3-month-old 18-month-old, male, Sprague–Dawley...