A5065635358- Telomeres, Telomerase, and Senescence
- Biochemical and Molecular Research
- Quinazolinone synthesis and applications
- Lung Cancer Treatments and Mutations
- Health, Environment, Cognitive Aging
- Animal Virus Infections Studies
- Colorectal Cancer Treatments and Studies
- Cancer Mechanisms and Therapy
- Spaceflight effects on biology
- RNA Interference and Gene Delivery
- Advanced Glycation End Products research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Redox biology and oxidative stress
- Lung Cancer Research Studies
- Neurological Disease Mechanisms and Treatments
- Birth, Development, and Health
- Retinoids in leukemia and cellular processes
- Genetics, Aging, and Longevity in Model Organisms
- Synthesis and Characterization of Heterocyclic Compounds
- Nitric Oxide and Endothelin Effects
- Advanced biosensing and bioanalysis techniques
- Barrier Structure and Function Studies
- Viral Infections and Immunology Research
- Nutrition, Genetics, and Disease
- Alkaline Phosphatase Research Studies
Instytut Biologii Doświadczalnej im. Marcelego Nenckiego
2011-2025
Polish Academy of Sciences
2016-2025
Abstract One promising strategy to alleviate aging symptoms is the treatment with senolytics that compounds which selectively eliminate senescent cells. Some therapies aim reduce of cellular senescence without cell eradication (senomorphic activity). However, senotherapies raise many questions concerning selectivity, safety and efficiency senolitic drugs. A vital question how senolytic affect young proliferating In our study, we checked impact quercetin dasatinib (D + Q), one drug mixtures...
Senescence is a stress response characterized by an irreversible growth arrest and alterations in certain cell functions. It believed that both double-strand DNA breaks (DSB) increased ROS level are the main culprit of senescence. Excessive production also particularly important development number cardiovascular disorders. In this context involvement professional ROS-producing enzymes, NADPH oxidases (NOX), was postulated. contrary to common knowledge, we have shown not only but diminished...
Abstract Torquetenovirus (TTV) viremia has been associated with increased mortality risk in the elderly population. This work aims to investigate TTV as a potential biomarker of immunosenescence. We compared levels circulating 1813 participants MARK-AGE project, including human models delayed (offspring centenarians [GO]) and premature (Down syndrome [DS]) The load was positively age, cytomegalovirus (CMV) antibody levels, Cu/Zn ratio negatively platelets, total cholesterol, IgM. highest DS...
Cells may undergo senescence in response to DNA damage, which is associated with cell cycle arrest, altered gene expression and morphology. Protein palmitoylation one of the mechanisms by damage regulated. Therefore, we hypothesized that protein played a role regulation senescent phenotype. Here, showed treatment human vascular smooth muscle cells (VSMCs) 2-bromopalmitate (2-BP), an inhibitor acyltransferases, changes different aspects phenotype, including resumption proliferation, decrease...
Women are living longer than men and it seems that one of the reasons could be better immune system females. In Poland, contrary to many European countries, women retire earlier men, namely at 60 65, respectively. We asked question how gender labour status were interconnected with some immunological parameters included in so called risk profile (IRP), such as CD4+/CD8+ ratio, percentage CD8+CD28−, NK, level circulating cytokines. A total 92 100 past retirement age, 65–74 years old, still...
Rational drug design and in vitro pharmacology profiling constitute the gold standard development pipelines. Problems arise, however, because this process is often difficult due to limited information regarding complete identification of a molecule's biological activities. The increasing affordability genome-wide next-generation technologies now provides an excellent opportunity understand compound's diverse effects on gene regulation. Here, we used unbiased approach lung colon cancer cell...
<title>Abstract</title> Cellular senescence, a permanent state of cell cycle arrest, can result either from external stress and is then called stress-induced premature senescence (SIPS), or the exhaustion division potential giving rise to replicative (RS). Despite numerous biomarkers distinguishing SIPS RS remains challenging. We propose claudin-1 (CLDN1) as cell-specific marker in vascular smooth muscle cells (VSMCs). In our study, VSMCs subjected exhibited significantly higher levels CLDN1...
The synergistic effect of trimetrexate (TMTX) and sulphonamide derivatives quinazoline on the cultured 5178Y murine leukemia cells was examined. On exposure to slightly inhibitory concentrations TMTX (0.1 nM) in combination with 2-desamino-2-methyl-10-propargyl-5,8-dideaza-pteroyl-sulphoglyc ine (DMPDDSF) (0.02 microM) a antifolates cell growth observed. These two drugs same caused also inhibition de novo synthesis thymidylate intact as measured by tritium release from...
The effect of thymidylate synthase inhibitors, fluorodeoxyuridine (FdUrd) and its two sulphonamide derivatives was examined in the culture murine leukemia cells -- 5178Y (parental subline) resistant subline 5178Y/F. A synergistic antimetabolites on cell survival observed exposure either line to a slightly inhibitory concentration FdUrd (1 nM) combination with 2-desamino-2-methyl-10-propargyl-5,8-dideaza-pteroylsulphogluta mate or...