A5015099842- Telomeres, Telomerase, and Senescence
- Curcumin's Biomedical Applications
- RNA Interference and Gene Delivery
- Genomics, phytochemicals, and oxidative stress
- DNA Repair Mechanisms
- Reproductive Biology and Fertility
- Retinoids in leukemia and cellular processes
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Advanced biosensing and bioanalysis techniques
- Sirtuins and Resveratrol in Medicine
- Neuroinflammation and Neurodegeneration Mechanisms
- Cell death mechanisms and regulation
- Assisted Reproductive Technology and Twin Pregnancy
- Gastroesophageal reflux and treatments
- Circadian rhythm and melatonin
- Diet and metabolism studies
- Epigenetics and DNA Methylation
- Gut microbiota and health
- Ovarian function and disorders
- Cancer therapeutics and mechanisms
- Skin Protection and Aging
- Nitric Oxide and Endothelin Effects
- Genetics, Aging, and Longevity in Model Organisms
- Autophagy in Disease and Therapy
- Genomics and Chromatin Dynamics
Instytut Biologii Doświadczalnej im. Marcelego Nenckiego
2015-2025
Polish Academy of Sciences
2013-2025
University of Warsaw
1993-2008
Polish Mother’s Memorial Hospital Research Institute
2007-2008
Senescence of vascular smooth muscle cells (VSMCs) contributes to aging as well age-related diseases the cardiovascular system. Senescent VSMCs have been shown be present in atherosclerotic plaques. Both replicative (RS) and stress-induced premature senescence (SIPS) accompany diseases. We aimed establish signature RS SIPS VSMCs, induced by a common anticancer drug, doxorubicin, discover so far undisclosed features senescent that are potentially harmful organism. Most hallmarks were for both...
Trimethylamine-N-oxide (TMAO) has been suggested as a marker and mediator of cardiovascular diseases. However, data are contradictory, the mechanisms obscure. Strikingly, role TMAO precursor trimethylamine (TMA) not drawn attention in studies even though toxic effects TMA were proposed several decades ago. We assessed plasma levels healthy humans (HH) patients qualified for aortic valve replacement (CP). The cytotoxicity rat cardiomyocytes was evaluated using an MTT test. on albumin lactate...
Aging | doi:10.18632/aging.203835. Adam Krzystyniak, Malgorzata Wesierska, Gregory Petrazzo, Agnieszka Gadecka, Magdalena Dudkowska, Anna Bielak-Zmijewska, Grazyna Mosieniak, Izabela Figiel, Jakub Wlodarczyk, Ewa Sikora
Abstract One promising strategy to alleviate aging symptoms is the treatment with senolytics that compounds which selectively eliminate senescent cells. Some therapies aim reduce of cellular senescence without cell eradication (senomorphic activity). However, senotherapies raise many questions concerning selectivity, safety and efficiency senolitic drugs. A vital question how senolytic affect young proliferating In our study, we checked impact quercetin dasatinib (D + Q), one drug mixtures...
// Wioleta Grabowska 1 , Małgorzata Suszek Maciej Wnuk 2 Anna Lewinska 3 Emilia Wasiak Ewa Sikora and Bielak-Zmijewska Department of Biochemistry, Nencki Institute Experimental Biology, Polish Academy Sciences, Warsaw, Poland Genetics, University Rzeszow, Rzeszów, Biochemistry Cell Correspondence to: Bielak-Zmijewska, email: Keywords : curcumin, senescence, sirtuins, VSMC, EC, Gerotarget Received January 26, 2016 Accepted March 23, Published 28, Abstract It is believed that a...
Abstract Curcumin is a natural pigment that has been shown to induce cell death in many cancer cells; however, the mode depends on type and curcumin concentration. Here we show that, Jurkat cells, 50 μmol/L severely lowers survival induces initial stage of chromatin condensation. It also caspase-3, which sufficient cleave DNA fragmentation factor 45 [DFF45/inhibitor caspase-activated DNase (ICAD)], inhibitor DFF40/CAD endonuclease. However, release from its does not lead oligonucleosomal...
Senescence is a stress response characterized by an irreversible growth arrest and alterations in certain cell functions. It believed that both double-strand DNA breaks (DSB) increased ROS level are the main culprit of senescence. Excessive production also particularly important development number cardiovascular disorders. In this context involvement professional ROS-producing enzymes, NADPH oxidases (NOX), was postulated. contrary to common knowledge, we have shown not only but diminished...
Abstract It has been suggested that trimethylamine oxide (TMAO), a liver oxygenation product of gut bacteria-produced (TMA), is marker cardiovascular risk. However, mechanisms the increase and biological effects TMAO are obscure. Furthermore, potential role precursor, TMA, not investigated. We evaluated effect age, risk factor, on plasma levels TMA TMAO, bacteria composition, gut-to-blood penetration histological hemodynamic parameters in 3-month-old 18-month-old, male, Sprague–Dawley...
The expected average human lifespan is constantly increasing. negative effect of this phenomenon more frequent incidences age-related diseases. Experimental data have shown that cellular senescence the cause organismal aging. Cellular an irreversible cell cycle arrest while maintaining metabolic functions and can occur through exhaustion proliferative potential (replicative - RS) or stress conditions (stress-induced premature SIPS). Both types a number morphological changes, in particular,...