A5076886309- S100 Proteins and Annexins
- Alkaline Phosphatase Research Studies
- Ion channel regulation and function
- Lipid Membrane Structure and Behavior
- Computational Drug Discovery Methods
- Drug Transport and Resistance Mechanisms
- ATP Synthase and ATPases Research
- Protease and Inhibitor Mechanisms
- Mitochondrial Function and Pathology
- Ion Transport and Channel Regulation
- Metabolism and Genetic Disorders
- Bone Metabolism and Diseases
- Trace Elements in Health
- Erythrocyte Function and Pathophysiology
- Lysosomal Storage Disorders Research
- Blood Coagulation and Thrombosis Mechanisms
- Vitamin D Research Studies
- Extracellular vesicles in disease
- Bone and Joint Diseases
- Vitamin K Research Studies
- Adipose Tissue and Metabolism
- Traumatic Brain Injury and Neurovascular Disturbances
- Nicotinic Acetylcholine Receptors Study
- Protein Structure and Dynamics
- Cellular transport and secretion
Instytut Biologii Doświadczalnej im. Marcelego Nenckiego
2015-2024
Polish Academy of Sciences
2012-2023
Université Claude Bernard Lyon 1
2003-2008
International Institute of Molecular and Cell Biology
2007
Centre National de la Recherche Scientifique
2002-2003
École d'Ingénieurs en Chimie et Sciences du Numérique
2003
University of Arkansas for Medical Sciences
1994-2000
Mercy Medical Center
1998-2000
The University of Texas at Arlington
2000
Mercy Hospital
1998-2000
Glutathione S-transferase P1-1 isoforms, differing in a single amino acid residue (Ile104 or Val104), have been previously identified human placenta [Ahmad, H., Wilson, D. E., Fritz, R. R., Singh, S. V., Medh, D., Nagle, G. T., Awasthi, Y. C. & Kurosky, A. (1990) Arch. Biochem. Biophys. 278, 398-408]. In the present report, enzymic properties of these two proteins are compared. [I104]glutathione has expressed from its cDNA Escherichia coli and purified to homogeneity by affinity...
Senescence of vascular smooth muscle cells (VSMCs) contributes to aging as well age-related diseases the cardiovascular system. Senescent VSMCs have been shown be present in atherosclerotic plaques. Both replicative (RS) and stress-induced premature senescence (SIPS) accompany diseases. We aimed establish signature RS SIPS VSMCs, induced by a common anticancer drug, doxorubicin, discover so far undisclosed features senescent that are potentially harmful organism. Most hallmarks were for both...
Active transport of conjugated and unconjugated electrophiles out cells is essential for cellular homeostasis. We have previously identified in human tissues a transporter, DNP-SG [S-(2,4-dinitrophenyl)glutathione] ATPase, capable carrying this function [Awasthi et al. (1998) Biochemistry 37, 5231−5238, 5239−5248]. now report the cloning ATPase. The sequence cDNA clone was identical to that RLIP76, known Ral-binding protein. RLIP76 expressed E. coli purified by affinity chromatography....
In bone, mineralization is tightly regulated by osteoblasts and hypertrophic chondrocytes which release matrix vesicles (MVs) control extracellular ionic conditions composition. MVs are the initial sites of hydroxyapatite (HA) mineral formation. Despite growing knowledge about their morphology function, biogenesis not well understood. The purpose this work was to determine source in osteoblast lineage, Saos-2 cells, check whether originated from microvilli. Microvilli were isolated apical...
Abstract Matrix vesicles (MVs) are extracellular organelles that initiate mineral formation, accumulating inorganic phosphate (P i ) and calcium leading to the formation of hydroxyapatite (HA) crystals, main component bones. MVs produced during bone as well endochondral calcification cartilage. released into matrix from osseous cells such osteoblasts hypertrophic chondrocytes. In this report, using 1‐D SDS‐PAGE, in‐gel tryptic digestion an LC‐MS‐MS/MS protein identification protocol, we...
The present review aims to systematically and critically analyze the current knowledge on phospholipases their role in physiological pathological mineralization undertaken by competent cells. Cellular lipid metabolism plays an important biological mineralization. mechanisms of are likely take place tissues other than bones teeth under specific conditions. For instance, vascular calcification arteries patients with renal failure, diabetes mellitus or atherosclerosis recapitulates bone...
The Ca2+-transporting ATPase (EC 3.6.1.38) of sarcoplasmic reticulum alternates between several conformational states during ATP-dependent Ca2+ transport.The El conformation is stabilized by 0.1 mM Ca2+and the E, vanadate in a Ca2+-free medium.Fourier transform infrared spectroscopy reveals significant differences two that indicate protein secondary structure.The and corresponding spectra can be interconverted reversibly changing concentration medium.The spectral changes appearance new...
Plant annexins, Ca(2+)- and membrane-binding proteins, are probably implicated in the cellular response to stress resulting from acidification of cytosol. To understand how annexins can contribute ion homeostasis, we investigated pH-induced changes structure function recombinant annexin AnnAt1 Arabidopsis thaliana. The decrease pH 7.0 5.8 reduced time formation channels by artificial lipid membranes 3.5 h 15-20 min increased their unitary conductance 32 63 pS. These were accompanied an...
Annexin A6 (AnxA6) is the largest member of annexin family proteins present in matrix vesicles (MVs). MVs are a special class extracellular that serve as nucleation site during cartilage, bone, and mantle dentin mineralization. In this study, we assessed localization AnxA6 MV membrane bilayer using native biomimetics. Biochemical analyses revealed can be divided into three distinct groups. The first group corresponds to Ca
Inorganic pyrophosphate is a potent inhibitor of bone mineralization by preventing the seeding calcium-phosphate complexes. Plasma cell membrane glycoprotein-1 and tissue nonspecific alkaline phosphatase were reported to be antagonistic regulators toward inorganic formation (by plasma glycoprotein-1) degradation phosphatase) under physiological conditions. In addition, they possess broad overlapping enzymatic functions. Therefore, we examined roles within matrix vesicles isolated from femurs...
Microcrystalline arrays of Ca2+-transporting ATPase (EC 3.6.1.38) develop in detergent-solubilized sarcoplasmic reticulum upon exposure to 10-20 mM CaCl2 at pH 6.0 for several weeks 2 degrees C, a crystallization medium that preserves the activity months. Of 48 detergents tested, optimal was obtained with Brij 36T, 56, and 96 detergent:protein weight ratio 4:1 octaethylene glycol dodecyl ether 2:1. Similar Ca2+-induced crystalline were purified or delipidated Ca2+-ATPase but lower ratios....
Annexins belong to a family of multi-functional membrane- and Ca(2+)-binding proteins. The characteristic feature these proteins is that they can bind membrane phospholipids in reversible, Ca(2+)-dependent manner. While animal annexins have been known for long time are fairly well characterized, their plant counterparts were discovered only 1989, tomato, not thoroughly studied yet. In the present review, we discuss available information about with special emphasis on biochemical functional...
Crystalline arrays of Ca2+-ATPase molecules develop in detergent-solubilized sarcoplasmic reticulum during incubation for several weeks at 2 degrees C under nitrogen a medium 0.1 M KCl, 10 mM K-3-(N-morpholino)propanesulfonate, pH 6.0, 3 MgCl2, 20 CaCl2, 20% glycerol, NaN3, 5 dithiothreitol, 25 IU/ml Trasylol, micrograms/ml 1,6-di-tert-butyl-p-cresol, mg/ml protein, and 2-4 mg detergent/mg protein. Electron microscopy sectioned, negatively stained, freeze-fractured, frozen-hydrated crystals...