A5088712369- Telomeres, Telomerase, and Senescence
- Curcumin's Biomedical Applications
- Genomics, phytochemicals, and oxidative stress
- Sirtuins and Resveratrol in Medicine
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Autophagy in Disease and Therapy
- Immune cells in cancer
- Advanced biosensing and bioanalysis techniques
- Virus-based gene therapy research
- Acute Myeloid Leukemia Research
- Hematopoietic Stem Cell Transplantation
- Plant Genetic and Mutation Studies
- Skin Protection and Aging
- T-cell and Retrovirus Studies
- DNA and Nucleic Acid Chemistry
- Nutrition, Genetics, and Disease
- Nanoplatforms for cancer theranostics
- Kruppel-like factors research
- Chronic Myeloid Leukemia Treatments
- Retinal Development and Disorders
- Cancer Immunotherapy and Biomarkers
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- Extracellular vesicles in disease
Instytut Biologii Doświadczalnej im. Marcelego Nenckiego
2013-2023
Polish Academy of Sciences
2013-2023
Senescence of vascular smooth muscle cells (VSMCs) contributes to aging as well age-related diseases the cardiovascular system. Senescent VSMCs have been shown be present in atherosclerotic plaques. Both replicative (RS) and stress-induced premature senescence (SIPS) accompany diseases. We aimed establish signature RS SIPS VSMCs, induced by a common anticancer drug, doxorubicin, discover so far undisclosed features senescent that are potentially harmful organism. Most hallmarks were for both...
// Wioleta Grabowska 1 , Małgorzata Suszek Maciej Wnuk 2 Anna Lewinska 3 Emilia Wasiak Ewa Sikora and Bielak-Zmijewska Department of Biochemistry, Nencki Institute Experimental Biology, Polish Academy Sciences, Warsaw, Poland Genetics, University Rzeszow, Rzeszów, Biochemistry Cell Correspondence to: Bielak-Zmijewska, email: Keywords : curcumin, senescence, sirtuins, VSMC, EC, Gerotarget Received January 26, 2016 Accepted March 23, Published 28, Abstract It is believed that a...
Chronic and acute myeloid leukemia evade immune system surveillance induce immunosuppression by expanding proleukemic Foxp3+ regulatory T cells (Tregs). High levels of immunosuppressive Tregs predict inferior response to chemotherapy, relapse, shorter survival. However, mechanisms that promote in leukemias remain largely unexplored. Here, we identify leukemic extracellular vesicles (EVs) as drivers effector Tregs. Using mouse model leukemia-like disease, found Rab27a-dependent secretion EVs...
Abstract Circulating miRNAs have potential as minimally invasive biomarkers for diagnosing various diseases, including ageing-related disorders such Alzheimer’s disease (AD). However, the lack of standardization in common analysis method, RT-qPCR, and specifically normalization step, has resulted inconsistent data across studies, hindering miRNA clinical implementation well basic research. To address this issue, study proposes an optimized protocol key steps profiling, which incorporates...
Brain and other nervous system cancers are the 10th leading cause of death worldwide. Genome instability, cell cycle deregulation, epigenetic mechanisms, cytoarchitecture disassembly, redox homeostasis as well apoptosis involved in carcinogenesis. A diet rich fruits vegetables is inversely related with risk developing cancer. Several studies report that cruciferous exhibited antiproliferative effects due to multi-pharmacological functions their secondary metabolites such isothiocyanate...
Curcumin, a phytochemical present in the spice named turmeric, and one of promising anti-aging factors, is itself able to induce cellular senescence. We have recently shown that cells building vasculature senesced as result curcumin treatment. Curcumin-induced senescence was DNA damage-independent; however, activation ATM observed. Moreover, neither increased ROS production, nor even were indispensable for progression. In this paper we tried elucidate mechanism curcumin-induced analyzed...
Cell senescence - an irreversible proliferation arrest is one of the possible cellular responses to stress. There a vast variety stimuli, extrinsic and intrinsic, known induce senescence, several molecular pathways involved in process; yet much still remains be explained. Senescent cells can communicate with neighboring through secreted factors such as cytokines chemokines. Several years ago it was shown that also more direct manner by exchange proteins via bridges (CBs). Recent studies show...
The search is ongoing for new anticancer therapeutics that would overcome resistance to chemotherapy. This includes chronic myeloid leukemia, particularly suitable the studies of novel compounds due its homogenous and well-known genetic background. Here we show efficacy dicarboximide denoted BK124.1 (C31H37ClN2O4) in a mouse CML xenograft model vitro two types chemoresistant cells: MDR1 blasts CD34+ patients' stem cells (N = 8) using immunoblotting flow cytometry. Intraperitoneal...
Abstract Background Circulating miRNAs in blood plasma present significant potential for use as minimally‐invasive biomarkers diagnosis of aging‐related diseases, such cancer or Alzheimer’s disease (AD). The most sensitive and inexpensive analysis method circulating miRNA is RT‐qPCR. However, lack RT‐qPCR standardisation one the major causes data inconsistency across different studies it hinders progress towards widespread implementation clinical use. Normalisation to stably expressed a key...
Abstract Background A number of late‐onset Alzheimer’s disease (AD) risk genes have been discovered, among which the most studied but not fully explored is apolipoprotein E gene ( APOE ). PICALM (coding Phosphatidylinositol Binding Clathrin Assembly Protein) another well‐recognized risk‐gene, shown to interact with in conferring AD risk. MicroRNAs (miRNAs) are post‐transcriptional regulators mRNAs, some known be involved pathology. The purpose this study was investigate middle‐aged, healthy...