A5025877757- Telomeres, Telomerase, and Senescence
- Amino Acid Enzymes and Metabolism
- Polyamine Metabolism and Applications
- Cannabis and Cannabinoid Research
- Aldose Reductase and Taurine
- Autophagy in Disease and Therapy
- Metabolism and Genetic Disorders
- RNA Interference and Gene Delivery
- Neuroinflammation and Neurodegeneration Mechanisms
- Circadian rhythm and melatonin
- Pharmacogenetics and Drug Metabolism
- Renal and related cancers
- Sexual Differentiation and Disorders
- Cancer-related Molecular Pathways
- CRISPR and Genetic Engineering
- Cellular transport and secretion
- Atherosclerosis and Cardiovascular Diseases
- Advanced Glycation End Products research
- Redox biology and oxidative stress
- Parvovirus B19 Infection Studies
- Viral Infections and Immunology Research
- Pancreatic function and diabetes
- Lung Cancer Research Studies
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Folate and B Vitamins Research
Instytut Biologii Doświadczalnej im. Marcelego Nenckiego
2008-2025
Polish Academy of Sciences
2002-2025
University of Dundee
2021-2022
Ninewells Hospital
2021-2022
Aging | doi:10.18632/aging.203835. Adam Krzystyniak, Malgorzata Wesierska, Gregory Petrazzo, Agnieszka Gadecka, Magdalena Dudkowska, Anna Bielak-Zmijewska, Grazyna Mosieniak, Izabela Figiel, Jakub Wlodarczyk, Ewa Sikora
Mitotic slippage (MS), the incomplete mitosis that results in a doubled genome interphase, is typical response of TP53-mutant tumors resistant to genotoxic therapy. These polyploidized cells display premature senescence and sort damaged DNA into cytoplasm. In this study, we explored MS MDA-MB-231 cell line treated with doxorubicin (DOX). We found selective release cytoplasm telomere fragments enriched telomerase reverse transcriptase (hTERT), capping protein TRF2, double-strand breaks marked...
The induction of senescence/polyploidization and their role in cancer recurrence is still a poorly explored issue. We showed that MDA-MB-231 MCF-7 breast cells underwent reversible upon pulse treatment with doxorubicin (dox). Subsequently, senescent/polyploid produced progeny (escapers) possessed the same amount DNA as parental cells. In dox-induced state, accumulation autophagy protein markers, such LC3B II p62/SQSTM1, was observed. However, senescent were characterized by very low rate new...
Abstract One promising strategy to alleviate aging symptoms is the treatment with senolytics that compounds which selectively eliminate senescent cells. Some therapies aim reduce of cellular senescence without cell eradication (senomorphic activity). However, senotherapies raise many questions concerning selectivity, safety and efficiency senolitic drugs. A vital question how senolytic affect young proliferating In our study, we checked impact quercetin dasatinib (D + Q), one drug mixtures...
Abstract Besides cell death, autophagy and senescence are the main outcomes of anticancer treatment. We demonstrate that tacrine-melatonin heterodimer C10, a potent anti-Alzheimer’s disease drug, has an antiproliferative effect on MCF-7 breast cancer cells. The response to 24 h-treatment with C10 was enhancement accompanied by inhibition mTOR AKT pathways. Significantly increased markers, such as LC3B- ATG16L-positive vesicles, confirmed induction C10. However, analysis autophagic flux using...
Abstract Torquetenovirus (TTV) viremia has been associated with increased mortality risk in the elderly population. This work aims to investigate TTV as a potential biomarker of immunosenescence. We compared levels circulating 1813 participants MARK-AGE project, including human models delayed (offspring centenarians [GO]) and premature (Down syndrome [DS]) The load was positively age, cytomegalovirus (CMV) antibody levels, Cu/Zn ratio negatively platelets, total cholesterol, IgM. highest DS...
Cells may undergo senescence in response to DNA damage, which is associated with cell cycle arrest, altered gene expression and morphology. Protein palmitoylation one of the mechanisms by damage regulated. Therefore, we hypothesized that protein played a role regulation senescent phenotype. Here, showed treatment human vascular smooth muscle cells (VSMCs) 2-bromopalmitate (2-BP), an inhibitor acyltransferases, changes different aspects phenotype, including resumption proliferation, decrease...
Women are living longer than men and it seems that one of the reasons could be better immune system females. In Poland, contrary to many European countries, women retire earlier men, namely at 60 65, respectively. We asked question how gender labour status were interconnected with some immunological parameters included in so called risk profile (IRP), such as CD4+/CD8+ ratio, percentage CD8+CD28−, NK, level circulating cytokines. A total 92 100 past retirement age, 65–74 years old, still...
The detection of antinuclear autoantibody (ANA) is dependent on many factors and varies between the populations. aim study was first to assess prevalence ANA in Polish adult population depending age, sex cutoff threshold used for results obtained. Second, we estimated occurrence individual types ANA-staining patterns. We tested 1731 patient samples using commercially available IIFA two thresholds 1:100 1:160. found 260 participants (15.0%), but percentage positive strongly depended level....
Rational drug design and in vitro pharmacology profiling constitute the gold standard development pipelines. Problems arise, however, because this process is often difficult due to limited information regarding complete identification of a molecule's biological activities. The increasing affordability genome-wide next-generation technologies now provides an excellent opportunity understand compound's diverse effects on gene regulation. Here, we used unbiased approach lung colon cancer cell...
The cross-talk in vivo between two signalling pathways activated by testosterone via intracellular androgen receptor, and induced damage to renal tubules evoked anti-folate [N10-propargyl-5,8-dideazafolic acid (CB 3717)] or folate is reported. We show that CB 3717/folate induces the expression of hepatocyte growth factor (HGF)/c-Met system injured kidneys which a significant, but transient, elevation HGF mRNA level occurs. It followed severalfold increase c-Met transmembrane receptor message...
Cross-talk between two signal transduction pathways leads to a negative regulation of androgen-induced ornithine decarboxylase (ODC) gene expression in the mouse kidney. One pathway is triggered by testosterone via intracellular androgen receptor, AR, and other induced antifolate CB 3717 or folate hepatocyte growth factor its cell membrane receptor c-Met. Here we report studies AR c-Myc transcription factors involved ODC transactivation. Administration decreased AR. In contrast, did not...
Polyamines play an important role in cell growth and differentiation, while their overproduction has potentially oncogenic consequences. Polyamine homoeostasis, a critical determinant of fate, is precisely tuned at the level biosynthesis, degradation transport. The enzymes ODC (ornithine decarboxylase), AdoMetDC (S-adenosylmethionine decarboxylase) SSAT (spermidine/spermine N1-acetyltransferase) are for polyamine pool maintenance. Our experiments were designed to examine expression these...
The cross-talk in vivo between two signalling pathways activated by testosterone via intracellular androgen receptor, and induced damage to renal tubules evoked anti-folate [N10-propargyl-5,8-dideazafolic acid (CB 3717)] or folate is reported. We show that CB 3717/folate induces the expression of hepatocyte growth factor (HGF)/c-Met system injured kidneys which a significant, but transient, elevation HGF mRNA level occurs. It followed severalfold increase c-Met transmembrane receptor message...
The anti-DFS70 autoantibodies are one of the most commonly and widely described agent unknown clinical significance, frequently detected in healthy individuals. It is not known whether DFS70 protective or pathogenic. One factors suspected inducing formation antibodies increased oxidative stress. We evaluated coexistence with selected markers stress investigated these could be considered as indirect stress.The intensity was measured all samples via indices free-radical damage to lipids...
A differential expression pattern of spermidine/spermine N(1)-acetyltransferase (SSAT), the enzyme critical to proper homeostasis cellular polyamines, is reported in mouse kidney undergoing hyperplasia and hypertrophy. We have shown that SSAT activity mRNA are significantly induced by antifolate CB 3717 folate evoke a drug-injury-dependent hyperplasia. In contrast, down-regulated testosterone-induced hypertrophic kidney, while positively controlled this androgen. Catecholamine depletion...