A5063747704- Metabolomics and Mass Spectrometry Studies
- Analytical Chemistry and Chromatography
- Microfluidic and Capillary Electrophoresis Applications
- Mass Spectrometry Techniques and Applications
- Computational Drug Discovery Methods
- Analytical Methods in Pharmaceuticals
- Chromatography in Natural Products
- Advanced Chemical Sensor Technologies
- Innovative Microfluidic and Catalytic Techniques Innovation
- RNA modifications and cancer
- Microbial Metabolic Engineering and Bioproduction
- Bladder and Urothelial Cancer Treatments
- Spectroscopy and Chemometric Analyses
- Metabolism and Genetic Disorders
- Ionic liquids properties and applications
- Urinary Tract Infections Management
- Advanced Drug Delivery Systems
- Protein purification and stability
- Molecular Biology Techniques and Applications
- Advanced Proteomics Techniques and Applications
- Biosensors and Analytical Detection
- Free Radicals and Antioxidants
- Cancer, Hypoxia, and Metabolism
- Hormonal and reproductive studies
- Gut microbiota and health
Gdańsk Medical University
2015-2024
Tbilisi State University
2024
Nicolaus Copernicus University
1997-2022
University of Warsaw
2022
University of Łódź
2022
Gdańsk University of Technology
2022
Adam Mickiewicz University in Poznań
2022
Silesian University of Technology
2022
Institute of Inorganic Chemistry of the Slovak Academy of Sciences
2022
Warsaw University of Technology
2022
Two types of chemically bonded phases for high-performance liquid chromatography (HPLC) have been prepared: a conventional C18 and AP (N-acylaminopropylsilica), novel one that contains specific interaction sites localized in the hydrophobic chain. Surface properties stationary phases, before after chemical modification, characterized by several physicochemical techniques, such as porosimetry, ICP atomic emission spectroscopy, elemental analysis, solid state CP/MAS NMR, chromatography. For...
Non-targeted metabolomics constitutes a part of systems biology and aims to determine many metabolites in complex biological samples. Datasets obtained non-targeted studies are multivariate high-dimensional due the sensitivity mass spectrometry-based detection methods as well complexity matrices. Proper selection variables which contribute into group classification is crucial step, especially focused on searching for disease biomarker candidates. In present study, three different statistical...
Plasma untargeted metabolomics is a common method for evaluation of the mechanisms underlying human pathologies and identification novel biomarkers. The plasma proteins provide environment transport hydrophobic metabolites. current sample preparation protocol relies on immediate precipitation thus leads to co-precipitation significant fraction Here we present new simple procedure that overcomes problem improves metabolome coverage. Introducing an additional step preceding protein...
The discovery of extracellular vesicles (EVs), including exosomes, microvesicles, and apoptotic bodies, has opened a new frontier in the study signal transduction understanding cell-to-cell communication. EVs play key role regulating various biological processes such as tissue regeneration, blood coagulation, immunomodulation or carcinogenesis. Therefore, they gain much attention potentially therapeutic non-invasive diagnostic targets. In this aspect, comprehensive characterization molecular...
Etodolac (ETD), a nonsteroidal anti-inflammatory drug, exhibits antinflammatory, analgesic, and antipyretic activity. The main type of ETD administration is oral route, which associated with significant systemic side effects. Nanostructured lipid carriers (NLC), modern formulation, are non-toxic, biocompatible, can improve the solubility stability drugs. (NLC) containing etodolac were prepared by melt-emulsification ultrasonication technique. Full factorial design (FFD) was applied to...
pH gradient reversed-phase HPLC consists of a programmed increase during the chromatographic run eluting power mobile phase with regard to ionizable analytes. On analogy conventional organic modifier RP HPLC, in mode, strength increases due its increasing (with acid analytes) or decreasing basic pH, whereas content is kept constant. We have shown previously that separations are technically possible using standard equipment. Here we demonstrate method uniquely suitable determine pKa values A...
Diagnosis of pulmonary arterial hypertension (PAH) is difficult due to the lack specific clinical symptoms and biomarkers, especially at early stages. We compared plasma metabolic fingerprints PAH patients (n = 20) with matched healthy volunteers using, for first time, untargeted multiplatform metabolomics approach consisting high-performance liquid gas chromatography coupled mass spectrometry. Multivariate statistical analyses were performed select metabolites that contribute most groups'...