A5034432360- Slime Mold and Myxomycetes Research
- Genomics and Chromatin Dynamics
- RNA Research and Splicing
- Genetic Mapping and Diversity in Plants and Animals
- Plant Stress Responses and Tolerance
- Diatoms and Algae Research
- Plant Molecular Biology Research
- Fungal and yeast genetics research
- Photosynthetic Processes and Mechanisms
- Biocrusts and Microbial Ecology
- Crop Yield and Soil Fertility
- Nuclear Structure and Function
- Plant responses to elevated CO2
- Protist diversity and phylogeny
- Legume Nitrogen Fixing Symbiosis
- Mitochondrial Function and Pathology
- Mycorrhizal Fungi and Plant Interactions
- Aquatic Ecosystems and Phytoplankton Dynamics
- Aquatic Ecosystems and Biodiversity
- Light effects on plants
- Echinoderm biology and ecology
- DNA and Biological Computing
- Erythrocyte Function and Pathophysiology
- Caveolin-1 and cellular processes
- Sphingolipid Metabolism and Signaling
University of Warsaw
2008-2020
Military Aviation Works No. 1 (Poland)
2019
University of Michigan–Ann Arbor
1990-1992
Maize, despite being thermophyllic due to its tropical origin, demonstrates high intraspecific diversity in cold-tolerance. To search for molecular mechanisms of this diversity, transcriptomic response cold was studied two inbred lines contrasting Microarray analysis followed by extensive statistical elaboration data, literature data mining, and gene ontology-based classification. The used had been bred earlier specifically determination QTLs cold-performance photosynthesis. This allowed...
Recent progress in selective breeding of maize (Zea mays L.) towards adaptation to temperate climate has allowed the production inbred lines withstanding cold springs with temperatures below 8 °C or even close 0 °C, indicating that despite its tropical origins is not inherently cold-sensitive. Here we studied acclimatory response three contrasting cold-sensitivity selected basing on multi-year routine field data. The observations were confirmed growth chamber. Under controlled conditions...
Maize is a cold-sensitive species, but selective breeding programs have recently succeeded in producing plants strikingly well adapted to the cold springs of temperate climate, showing potential for improved tolerance. The aim present study was determine whether adaptation some inbred lines spring chills due their increased true tolerance or it only represents an avoidance mechanism, which sole mode during early stages agricultural dispersal maize towards higher latitudes. By characterizing...
In maize seedlings, severe cold results in dysregulation of circadian pattern gene expression causing profound modulation transcription genes related to photosynthesis and other key biological processes. Plants live highly cyclic life their response environmental stresses must allow for underlying rhythms. To study the interplay a stress rhythmic cue we investigated transcriptomic seedlings low temperature context diurnal expression. Severe had pronounced effect on rhythm substantial...
Maize is a cold-sensitive plant whose physiological reactions to sub-optimal temperatures are well understood, but their molecular foundations only beginning be deciphered. In an attempt identify key genes involved in these reactions, we surveyed several independent transcriptomic studies addressing the response of juvenile maize moderate or severe cold. Among tens thousands found change expression upon cold treatment less than 500 were reported more one study, indicating astonishing...
The developmentally regulated sea urchin early histone gene repeat (SUEHGR) from Strongylocentrotus purpuratus was isolated as chromatin by nucleoprotein hybridization.This technique is a novel method to isolate specific sequences chromatin.Because the purification scheme based only on sequence and independent of other physical properties such protein composition transcriptional activity, we were able same in different functional states.Gene size fragments solubilized restriction...
Background Numerous biochemical and physiological parameters of living organisms follow a circadian rhythm. Although such rhythmic behavior is particularly pronounced in plants, which are strictly dependent on the daily photoperiod, data molecular aspects diurnal cycle plants scarce mostly concerns model species Arabidopsis thaliana. Here we studied leaf transcriptome seedlings maize, an important C4 crop only distantly related to A. thaliana, throughout 10 h darkness 14 light look for...
Abstract Populations of three sympatric species (Daphnia cucullata, Daphnia galeata, and hyalina longispina complex)) inhabiting lakes in northeastern Poland (Roś, Mikołajskie, Wigry) were checked for interspecific hybrids. Hybrid coexisting with parental individuals detected each the investigated lakes. Several classes hybrids (F1, F2, backcrosses), including D. cucullata×galeata×hyalina individuals, have been detected. Hybrids constituted a substantial (13%–38%) part total abundance...
Background Human SA/STAG proteins, homologues of the yeast Irr1/Scc3 cohesin, are least studied constituents sister chromatid cohesion complex crucial for proper chromosome segregation. The two SA paralogues, SA1 and SA2, show some specificity towards region they stabilize, but not SA1, has been shown to participate in transcriptional regulation as well. molecular basis this functional divergence is unknown. Methodology/Principal Findings In silico analysis indicates numerous putative...
Journal Article Chromatin structure of the developmentally regulated early histone genes sea urchin Strongylocentrotus purpuratus Get access Jan Fronk, Fronk Biophysics Research Division and Department Biological Sciences, University MichiganAnn Arbor, MI 48109-2099, USA Search for other works by this author on: Oxford Academic PubMed Google Scholar Graeme A. Tank, Tank John P. Langmore * *To whom correspondence should be addressed Nucleic Acids Research, Volume 18, Issue 17, 11 September...
Abstract Murine DNA methyltransferases Dnmt1 and Dnmt3a were expressed in the yeast Saccharomyces cerevisiae . Adjustment to preferences of nucleotide sequences upstream downstream translation initiation sites both cDNAs was needed obtain significant levels methyltransferases. Both proteins correctly localized nucleus their presence had no measurable influence on functioning cells. cells enzymatically active vitro , vivo genomic transgenic S. ca. 0.06% 0.4%, respectively, cytosines became...
Cytosine methylation of developmentally regulated genes the sea urchin Strongylocentrotus purpuratus was studied by using restriction-endonuclease digestion and Southern blotting. The single-copy bindin gene, family five cytoplasmic actin 400-fold-repeated set early histone were mostly unmethylated, but some sites exhibited partial that varied throughout development. This shows in echinoderms DNA is not confined to non-transcribed portion genome, as previously believed [Bird, Tagart &...
Glutathione homeostasis is crucial for cell functioning. We describe a novel Imi1 protein of Saccharomyces cerevisiae affecting mitochondrial integrity and involved in controlling glutathione level. cytoplasmic and, except its N-terminal Flo11 domain, has distinct solenoid structure. A lack leads to lesions comprising aberrant morphology cristae multifarious mtDNA rearrangements impaired respiration. The malfunctioning coupled significantly decrease the level intracellular reduced without...
The sister chromatid cohesion complex of Saccharomyces cerevisiae is composed proteins termed cohesins. forms a ring structure that entraps DNAs, probably following replication. mechanism universal and the participating in this process are evolutionarily highly conserved. We investigated Irr1p/Scc3p cohesin subunit, an under-studied protein. show presence mutated copy IRR1 gene, encoding F658G substitution Irr1p, changes sensitivity heterozygous irr1-1/IRR1 diploid to cell wall-affecting...
Starvation-induced differentiation of the slime mould Physarum polycephalum is accompanied by continuous methylation DNA. No stable changes in overall level DNA are evident, but a gene known to be transcribed specifically during subject increased methylation. Inhibitors preclude P. polycephalum, although they only marginally inhibitory normal growth. Taken together these results indicate that involved this lower eukaryote.