A5064506756- Peroxisome Proliferator-Activated Receptors
- Biosimilars and Bioanalytical Methods
- Circadian rhythm and melatonin
- Mesenchymal stem cell research
- Metabolism, Diabetes, and Cancer
- Glycogen Storage Diseases and Myoclonus
- Autophagy in Disease and Therapy
- Pancreatic function and diabetes
- Biomedical Research and Pathophysiology
- Adenosine and Purinergic Signaling
- Ion Transport and Channel Regulation
- Monoclonal and Polyclonal Antibodies Research
- Inflammatory mediators and NSAID effects
- Heme Oxygenase-1 and Carbon Monoxide
- Cancer, Lipids, and Metabolism
- Muscle Physiology and Disorders
- Nutrition, Genetics, and Disease
- Cholinesterase and Neurodegenerative Diseases
- Cell Image Analysis Techniques
- Biochemical effects in animals
- Angiogenesis and VEGF in Cancer
- Nerve injury and regeneration
- Chronic Kidney Disease and Diabetes
- Cancer, Hypoxia, and Metabolism
- Gastroesophageal reflux and treatments
SciencePharma (Poland)
2021-2024
Medtronic (Ireland)
2019
Adamed (Poland)
2007-2015
University of Warsaw
1998-2013
Institute of Biochemistry and Biophysics, Polish Academy of Sciences
2003-2011
Heme oxygenase-1 (HMOX1) is a cytoprotective enzyme degrading heme to biliverdin, iron ions, and carbon monoxide, whose expression induced in response oxidative stress. Its overexpression has been suggested as strategy improving survival of transplanted muscle precursors.Here we demonstrated that HMOX1 inhibits differentiation myoblasts modulates miRNA processing: downregulates Lin28 DGCR8, lowers the total pool cellular miRNAs, specifically blocks induction myomirs. Genetic or...
Impaired wound healing in diabetes is related to decreased production of growth factors. Hence, gene therapy considered as promising treatment modality. So far, efforts concentrated on single with particular emphasis vascular endothelial factor-A (VEGF-A). However, multiple proteins are involved this process it rational test new approaches. Therefore, the aim study was investigate whether AAV vector-mediated simultaneous transfer VEGF-A and fibroblast factor 4 (FGF4) coding sequences will...
Anserine (beta-alanyl-N(Pi)-methyl-L-histidine), a naturally occurring derivative of carnosine (beta-alanyl-L-histidine), is an abundant constituent skeletal muscles and brain many vertebrates. Although it has long been proposed to serve as proton buffer, radicals scavenger transglycating agent, its physiological function remains obscure. The formation anserine catalyzed by N-methyltransferase which exhibits unknown molecular identity. In the present investigation, we have purified from...
Abstract Introduction Diabetes is associated with reduced expression of heme oxygenase-1 (HO-1), a heme-degrading enzyme cytoprotective and proangiogenic properties. In myoblasts muscle satellite cells HO-1 improves survival, proliferation production growth factors. Induction in injured tissues facilitates neovascularization, the process impaired diabetes. We aimed to examine whether conditioned media from overexpressing myoblast cell line can improve blood-flow recovery ischemic muscles...
The effect of melatonin on glucose metabolism in the presence and absence insulin has been investigated primary cultures renal tubules grown a defined medium. In medium containing 5 microg/mL 2 mm alanine + glycerol 0.5 octanoate, 100 nm stimulated both lactate synthesis, while devoid action was negligible. Melatonin-induced increase synthesis accompanied by an enhancement consumption. view measurements [U-14C]L-alanine [U-14C]L-glycerol incorporation into glucose, it is likely that...
Peroxisome proliferator-activated receptor-γ (PPARγ) agonists, which have been used as insulin sensitizers in diabetic patients, may improve functions of endothelial cells (ECs). We investigated the effect PPARγ on angiogenic activities murine ECs and bone marrow-derived proangiogenic (PACs). PACs were isolated from marrow 10-12 weeks old, wild type, db/db heterozygous animals. Cells cultured fibronectin gelatin coated dishes EGM-2MV medium. For vitro stimulations, rosiglitazone (10 μmol/L)...
The antioxidative effects of melatonin (Mel), 5-hydroxytryptophan (5-HTP) and taurine (TAU) on hyperglycemia-induced oxidative stress was investigated in primary cultures kidney-cortex tubule cells grown metabolically hormonally defined medium. In the presence 30 mm glucose (hyperglycemic conditions), cell viability decreased by about 35% comparison with that estimated glucose-depleted medium probably as a result induction apoptosis, concluded from: (i) chromatin condensation DNA...
Therapeutic effect of rosiglitazone has been reported to result from an improvement insulin sensitivity and inhibition glucose synthesis. As the latter process occurs in both liver kidney cortex aim this study was elucidate action on formation tissues. Primary cultured cells grown defined medium were use throughout. To identify mechanism responsible for drug-induced changes, intracellular gluconeogenic intermediates enzyme activities determined. In contrast hepatocytes, administration a 10...
Effects of equimolar concentrations proinsulin C-peptide and insulin on glucose synthesis were studied in primary cultures rabbit kidney-cortex tubules grown the presence alanine, glycerol, octanoate. The rhodamine-labeled entered renal tubular cells localized nuclei, both absence insulin; preincubations with unlabeled compound inhibited internalization. did not affect formation when added alone but potentiated inhibitory action by about 20% due to a decrease flux through glucose-6-phosphate...
Abstract Murine DNA methyltransferases Dnmt1 and Dnmt3a were expressed in the yeast Saccharomyces cerevisiae . Adjustment to preferences of nucleotide sequences upstream downstream translation initiation sites both cDNAs was needed obtain significant levels methyltransferases. Both proteins correctly localized nucleus their presence had no measurable influence on functioning cells. cells enzymatically active vitro , vivo genomic transgenic S. ca. 0.06% 0.4%, respectively, cytosines became...
The effect of ethanol on glucose synthesis in kidney-cortex tubules control and diabetic rabbits has been investigated.Both freshly isolated grown primary cultures, were incubated with alanine or aspartate plus lactate glycerol octanoate the absence presence 100 mmol/l ethanol.In renal octanoate, culture medium containing alcohol, resulted about 30% decrease formation. A diminished production was accompanied by: (i) a utilization, (ii) an increase consumptions (iii) decline GSH:GSSG ratio....
Natalizumab is a recombinant, humanized form of monoclonal antibody that binds to CD49d. The presented study was conducted explore the suitability Göttingen pigs as pharmacokinetic/pharmacodynamic model in preclinical phase biosimilar natalizumab development. minipigs were treated with 1.286 or 3.0 mg (Tysabri®) per kg body weight by single 1-hour intravenous infusion. Six days before (baseline) and 30 after administration dose natalizumab, blood samples taken for analysis. No signs local...
Peroxisome proliferator-activated receptor-γ (PPARγ) agonists, which have been used as insulin sensitizers in diabetic patients, may improve functions of endothelial cells (ECs). We investigated the effect PPARγ on angiogenic activities murine ECs and bone marrow-derived proangiogenic (PACs). PACs were isolated from marrow 10-12 weeks old, wild type, db/db heterozygous animals. Cells cultured fibronectin gelatin coated dishes EGM-2MV medium. For vitro stimulations, rosiglitazone (10 μmol/L)...
Background Due to multiple similarities in the structure and physiology of human pig skin, model is extremely useful for biological drug testing after subcutaneous administration. Knowledge differences between injection sites could have a significant impact on absorption phase pharmacokinetic profiles drugs. Objectives This study aimed analyze administration site pharmacokinetics selected biochemical hematological parameters single ustekinumab pigs. Drug concentrations blood plasma were...