A5025879395- Heme Oxygenase-1 and Carbon Monoxide
- Angiogenesis and VEGF in Cancer
- Cancer, Hypoxia, and Metabolism
- Virus-based gene therapy research
- High Altitude and Hypoxia
- Eicosanoids and Hypertension Pharmacology
- Mesenchymal stem cell research
- RNA Interference and Gene Delivery
- Viral Infections and Immunology Research
- Thermal Regulation in Medicine
- Agriculture, Plant Science, Crop Management
- Viral Infectious Diseases and Gene Expression in Insects
- Cardiac Arrest and Resuscitation
- CAR-T cell therapy research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Tissue Engineering and Regenerative Medicine
- Cancer, Lipids, and Metabolism
- Engine and Fuel Emissions
- CRISPR and Genetic Engineering
- Botany and Plant Ecology Studies
- Cardiac Fibrosis and Remodeling
- Pesticide Residue Analysis and Safety
- Sarcoidosis and Beryllium Toxicity Research
- Genomics, phytochemicals, and oxidative stress
- Muscle Physiology and Disorders
Jagiellonian University
2015-2024
Rzeszów University
2008-2013
Biomedical Research Networking Center on Neurodegenerative Diseases
2010-2011
Centro de Investigación Biomédica en Red
2010
Consejo Superior de Investigaciones Científicas
2010
Universidad Autónoma de Madrid
2010
Hospital La Paz Institute for Health Research
2010
Current therapies for motor symptoms of Parkinson's disease (PD) are based on dopamine replacement. However, the progression remains unaffected, because continuous dopaminergic neuron loss. Since oxidative stress is actively involved in neuronal death PD, pharmacological targeting antioxidant machinery may have therapeutic value. Here, we analyzed relevance phase II response mediated by transcription factor NF-E2-related 2 (Nrf2) brain protection against parkinsonian toxin...
Background The transcription factor Nrf2 (NF-E2-related 2) and its target gene products, including heme oxygenase-1 (HO-1), elicit an antioxidant response that may have therapeutic value for Parkinson's disease (PD). However, HO-1 protein levels are increased in dopaminergic neurons of (PD) patients, suggesting participation free-iron deposition, oxidative stress neurotoxicity. Before targeting PD therapy it is imperative to determine if neurotoxic or neuroprotective the basal ganglia....
Nuclear factor E2-related 2 (Nrf2), a key cytoprotective transcription factor, regulates also proangiogenic mediators, interleukin-8 and heme oxygenase-1 (HO-1). However, hitherto its role in blood vessel formation was modestly examined. Particularly, although Nrf2 shown to affect hematopoietic stem cells, it not tested bone marrow-derived cells (PACs). Here we investigated angiogenic properties of PACs, endothelial inflammation-related revascularization.Treatment with cytokines increased...
Heme oxygenase-1 (HMOX1) is a cytoprotective enzyme degrading heme to biliverdin, iron ions, and carbon monoxide, whose expression induced in response oxidative stress. Its overexpression has been suggested as strategy improving survival of transplanted muscle precursors.Here we demonstrated that HMOX1 inhibits differentiation myoblasts modulates miRNA processing: downregulates Lin28 DGCR8, lowers the total pool cellular miRNAs, specifically blocks induction myomirs. Genetic or...
Through hypoxia-inducible factor 1 (HIF-1), hypoxia regulates the expression of numerous genes and is a potent inducer angiogenesis. However, interleukin-8 (IL-8), an important angiogenic mediator, has been reported to be downregulated by HIF-1, although mechanisms have not elucidated. HIF-1 was induced in human endothelial cells dimethyloxaloylglycine (DMOG). Interestingly, both DMOG attenuated IL-8 expression, similar effect obtained adenoviral overexpression stable form HIF-1alpha. Heme...
The pleiotropic effects of statins, inhibitors 3-hydroxy-3-methylglutaryl–coenzyme A (HMG-CoA) reductase, have been recently extended to the modulation angiogenesis. Here, get more insight into statins action, authors investigated effect atorvastatin on expression several angiogenic and inflammatory genes in human umbilical endothelial cells (HUVECs). Atorvastatin was proangiogenic at dose 10 nM, antiangiogenic concentrations 1 μ M. Moreover, these higher inhibited also proliferation HUVECs...
Heme oxygenase-1 (HO-1) is a cytoprotective enzyme that can be down-regulated in diabetes. Its importance for mature endothelium has been described, but its role proangiogenic progenitors not well known. We investigated the effect of HO-1 on angiogenic potential bone marrow-derived cells (BMDCs) and blood flow recovery ischemic muscle diabetic mice.Lack decreased number endothelial progenitor (Lin(-)CD45(-)cKit(-)Sca-1(+)VEGFR-2(+)) murine marrow, inhibited cultured BMDCs, affecting their...
Impaired wound healing in diabetes is related to decreased production of growth factors. Hence, gene therapy considered as promising treatment modality. So far, efforts concentrated on single with particular emphasis vascular endothelial factor-A (VEGF-A). However, multiple proteins are involved this process it rational test new approaches. Therefore, the aim study was investigate whether AAV vector-mediated simultaneous transfer VEGF-A and fibroblast factor 4 (FGF4) coding sequences will...
AimsHaem oxygenase-1 (HO-1) is a haem-degrading enzyme that generates carbon monoxide, bilirubin, and iron ions. Through these compounds, HO-1 mitigates cellular injury by exerting antioxidant, anti-apoptotic, anti-inflammatory effects. Here, we examined the influence of deficiency transient hypoxia/ischaemia-induced overexpression on post-injury hindlimb recovery.
Abstract Aims Angiotensin (Ang) II signalling has been suggested to promote cardiac fibrosis in inflammatory heart diseases; however, the underlying mechanisms remain obscure. Using Agtr1a-/- mice with genetic deletion of angiotensin receptor type 1 (ATR1) and experimental autoimmune myocarditis (EAM) model, we aimed elucidate role Ang II-ATR1 pathway development heart-specific autoimmunity post-inflammatory fibrosis. Methods results EAM was induced wild-type (WT) by subcutaneous injections...
Heme oxygenase-1 (Hmox1) is a stress-inducible protein crucial in heme catabolism. The end products of its enzymatic activity possess anti-oxidative, anti-apoptotic and anti-inflammatory properties. Cardioprotective effects Hmox1 were demonstrated experimental models myocardial infarction (MI). Nevertheless, importance timely resolution post-ischemic inflammation remains incompletely understood. aim this study was to determine the role monocyte/macrophage-mediated cardiac remodeling mouse...
Cell therapies are extensively tested to restore heart function after myocardial infarction (MI). Survival of any cell type intracardiac administration, however, may be limited due unfavorable conditions damaged tissue. Therefore, the aim this study was evaluate therapeutic effect adipose-derived stromal cells (ADSCs) and human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) overexpressing either proangiogenic SDF-1α or anti-inflammatory heme oxygenase-1 (HO-1) in a murine...
Abstract Aims Tumour necrosis factor α (TNF-α) represents a classical pro-inflammatory cytokine, and its increased levels positively correlate with the severity of many cardiovascular diseases. Surprisingly, some heart failure patients receiving high doses anti-TNF-α antibodies showed serious health worsening. This work aimed to examine role TNF-α signalling on development progression myocarditis heart-specific autoimmunity. Methods results Mice genetic deletion (Tnf+/− Tnf−/−) littermate...
Abstract Aging is the most important risk factor for development of cardiovascular diseases. Senescent cells release plethora factors commonly known as senescence-associated secretory phenotype, which can modulate normal function vascular wall. It currently not well understood if and how endothelial cell senescence affect adventitial niche. The aim this study was to characterize oxidative stress-induced identify their paracrine effects on primary type adventitia, fibroblasts. Human aortic...
Heart-specific CD4+ T cells have been implicated in development and progression of myocarditis mice humans. Here, using mouse models experimental autoimmune (EAM) we investigated the role heart non-specific disease. Heart were obtained from DO11.10 expressing transgenic cell receptor recognizing chicken ovalbumin. We found that infiltrating expressed exclusively effector (Teff) phenotype EAM model hearts patients with lymphocytic myocarditis. Adoptive transfer experiments showed while...