A5018278246- Cardiac Fibrosis and Remodeling
- Viral Infections and Immunology Research
- Systemic Sclerosis and Related Diseases
- Pluripotent Stem Cells Research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Tissue Engineering and Regenerative Medicine
- Pancreatic function and diabetes
- T-cell and B-cell Immunology
- Cell Adhesion Molecules Research
- Atherosclerosis and Cardiovascular Diseases
- Medical Imaging and Pathology Studies
- Connective Tissue Growth Factor Research
- Congenital heart defects research
- Mast cells and histamine
- Peptidase Inhibition and Analysis
- Cardiomyopathy and Myosin Studies
- Signaling Pathways in Disease
- Immune Response and Inflammation
- Eicosanoids and Hypertension Pharmacology
- Monoclonal and Polyclonal Antibodies Research
- Autophagy in Disease and Therapy
- CAR-T cell therapy research
- Dermatologic Treatments and Research
- Cardiovascular Function and Risk Factors
- Cardiovascular Effects of Exercise
University Hospital of Zurich
2009-2024
University of Zurich
2011-2024
Jagiellonian University
2016-2024
Uniwersytecki Szpital Dziecięcy
2019-2023
University of Agriculture in Krakow
2017
GZO Spital Wetzikon
2011-2016
University of Basel
2015
Swiss Integrative Center for Human Health
2009-2013
Resonance Research (United States)
2013
Albert Einstein College of Medicine
2013
Mouse embryonic stem (ES) cells differentiate into of all three primary germ layers including endodermal that produce insulin in vitro . We show constitutive expression Pax4 (Pax4 + ), and to a lesser extent Pdx1 (Pdx1 affects the differentiation ES significantly promote development insulin-producing cells. In overexpressing R1 cells, isl-1, ngn3, insulin, islet amyloid polypeptide, glucose transporter 2 (Glut-2) mRNA levels increase significantly. The number nestin-expressing (nestin+) also...
Mouse embryonic stem (ES) cells were used as an experimental model to study the effects of electromagnetic fields (EMF). ES-derived nestin-positive neural progenitor exposed extremely low frequency EMF simulating power line magnetic at 50 Hz (ELF-EMF) and radiofrequency Global System for Mobile Communication (GSM) signals 1.71 GHz (RF-EMF). Following exposure, analyzed transcript levels cell cycle regulatory, apoptosis-related, neural-specific genes proteins; changes in proliferation;...
Myocardial fibrosis critically contributes to cardiac dysfunction in inflammatory dilated cardiomyopathy (iDCM). Activation of transforming growth factor-β (TGF-β) signalling is a key-step promoting tissue remodelling and iDCM. Downstream mechanisms controlling these processes, remain elusive.Experimental autoimmune myocarditis (EAM) was induced BALB/c mice with heart-specific antigen adjuvant. Using heart-inflammatory precursors, as well mouse human fibroblasts, we demonstrated rapid...
Regulatory T cells (Tregs) represent a major population in the control of immune homeostasis and autoimmunity. Here we show that Fos-like 2 (Fosl2), TCR-induced AP1 transcription factor, represses Treg development controls Mice overexpressing Fosl2 (Fosl2tg) indeed systemic inflammatory phenotype, with infiltrates multiple organs. This phenotype is absent Fosl2tg × Rag2−/− mice lacking B cells, induces cell-intrinsic reduction responsible for phenotype. can transfer inflammation, which...
Abstract Experimental autoimmune myocarditis (EAM) represents a Th17 T cell-mediated mouse model of postinflammatory heart disease. In BALB/c wild-type mice, EAM is self-limiting disease, peaking 21 days after α-myosin H chain peptide (MyHC-α)/CFA immunization and largely resolving thereafter. IFN-γR−/− however, exacerbated shows chronic progressive disease course. We found that this course paralleled persistently elevated IL-17 release from cells infiltrating the hearts mice 30...
Prominin-1/CD133 is a plasma membrane marker found in several types of somatic stem cells, including hematopoietic and neural cells. To study its role during development with differentiation, we analyzed temporal spatial expression (mRNA protein) preimplantation embryos, undifferentiated mouse embryonic (ES) differentiated ES cell progeny. In early prominin-1 was expressed trophoblast but not cells the inner mass; however, transcripts were detected Both ES-derived committed to...
The myeloid differentiation factor (MyD)88/interleukin (IL)-1 axis activates self-antigen-presenting cells and promotes autoreactive CD4(+) T-cell expansion in experimental autoimmune myocarditis, a mouse model of inflammatory heart disease.The aim this study was to determine the role MyD88 IL-1 progression acute myocarditis an end-stage failure.Using alpha-myosin heavy chain peptide (MyHC-alpha)-loaded, activated dendritic cells, we induced wild-type MyD88(-/-) mice with similar...
WNT signaling plays an important role in fibrotic processes the heart. Recently, exosomes have been proposed as novel extracellular transporters for proteins. In this study, we analyzed whether WNT3a and WNT5a carried by could activate downstream molecular pathways human cardiac fibroblasts. Exosomes were isolated from conditioned medium of control, WNT3a- WNT5a-producing L cells differential ultracentrifugations. Obtained showed size ranging between 20⁻150 nm expressed exosomal markers...
Nuclear receptors and their cofactors regulate key pathophysiological processes in atherosclerosis development. The transcriptional activity of these nuclear is controlled by the receptor corepressors (NCOR), scaffolding proteins that form basis large corepressor complexes. Studies with primary macrophages demonstrated deletion Ncor1 increases expression atherosclerotic molecules. However, role atherogenesis unknown.We generated myeloid cell-specific knockout mice crossbred them low-density...
TGF-β is a master regulator of fibrosis, driving the differentiation fibroblasts into apoptosis-resistant myofibroblasts and sustaining production extracellular matrix (ECM) components. Here, we identified nuclear long noncoding RNA (lncRNA) H19X as TGF-β-driven tissue fibrosis. was consistently upregulated in wide variety human fibrotic tissues diseases strongly induced by TGF-β, particularly fibroblast-related cells. Functional experiments following silencing revealed that an obligatory...
Cardiac fibrosis is a pathological process associated with the development of heart failure. TGF-β and WNT signaling have been implicated in pathogenesis cardiac fibrosis, however, little known about molecular cross-talk between these two pathways. The aim this study was to examine effect exogenous canonical WNT3a non-canonical WNT5a TGF-β-activated human fibroblasts. We found that induced β-catenin-dependent response, whereas prompted AP-1 activity. triggered profibrotic signatures...
Background Systemic sclerosis (SSc) is an autoimmune disease characterized by overproduction of extracellular matrix (ECM) and multiorgan fibrosis. Animal studies pointed to bone marrow-derived cells as a potential source pathological ECM-producing in immunofibrotic disorders. So far, involvement monocytes macrophages the fibrogenesis SSc remains poorly understood. Methods Results Immunohistochemistry analysis showed accumulation CD14 + collagen-rich areas, well increased amount alpha smooth...
The antioxidant and anti-inflammatory activities of acylated decarboxylated gomphrenins, as well Basella alba L. fruit extract, were investigated in relation to gomphrenin, known for its high biological potential. most abundant natural namely, 6′-O-E-caffeoyl-gomphrenin (malabarin) 6′-O-E-4-coumaroyl-gomphrenin (globosin), isolated from B. extract the studies. In addition, controlled thermal decarboxylation gomphrenin purified at 65–75 °C resulted formation prevalent products, including...
We present a new strategy for the differentiation of embryonic stem (ES) cells into insulin-producing via multi-step process without selection and induction nestin-positive cells. During ES cell differentiation, transcript levels genes characteristic early mature beta including Pdx1, Pax4, insulin islet amyloid pancreatic peptide are up regulated. Islet-like clusters characterized by expression C-peptide, partially cytokeratin 19 as well ion channel activity similar to that found in Cells...
Rationale : Myocardial fibrosis is a hallmark of inflammation-triggered end-stage heart disease, common cause failure in young patients. Objective We used CD4 + T-cell–mediated experimental autoimmune myocarditis model to determine the parameters regulating cardiac inflammatory disease. Methods and Results α-Myosin heavy chain peptide/complete Freund’s adjuvant immunization was induce BALB/c mice. Chimeric mice, reconstituted with enhanced green fluorescence protein (EGFP) bone marrow, were...
The goal of this study was to characterize the performance fluorine-19 ((19)F) cardiac magnetic resonance (CMR) for specific detection inflammatory cells in a mouse model myocarditis. Intravenously administered perfluorocarbons are taken up by infiltrating and can be detected (19)F-CMR. (19)F-labeled should, therefore, generate an exclusive signal at inflamed regions within myocardium.Experimental autoimmune myocarditis induced BALB/c mice. After intravenous injection 2×200 µL...
Supernatants of serum-free cultured mononuclear cells (MNC) contain a mix immunomodulating factors (secretome), which have been shown to attenuate detrimental inflammatory responses following myocardial ischaemia. Inflammatory dilated cardiomyopathy (iDCM) is common cause heart failure in young patients. Experimental autoimmune myocarditis (EAM) CD4+ T cell-dependent model, mirrors important pathogenic aspects iDCM. The aim this study was determine the influence MNC secretome on inflammation...