A5020144166- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Cancer Immunotherapy and Biomarkers
- Neuroendocrine Tumor Research Advances
- Virus-based gene therapy research
- Cancer Genomics and Diagnostics
- Vasculitis and related conditions
- Cervical Cancer and HPV Research
- Cutaneous Melanoma Detection and Management
- T-cell and B-cell Immunology
- Melanoma and MAPK Pathways
- Neurotransmitter Receptor Influence on Behavior
- Lung Cancer Research Studies
- Receptor Mechanisms and Signaling
- Eosinophilic Disorders and Syndromes
- Atherosclerosis and Cardiovascular Diseases
- Cancer Research and Treatments
- Head and Neck Surgical Oncology
- Immune Cell Function and Interaction
- Single-cell and spatial transcriptomics
- Sarcoma Diagnosis and Treatment
- Glycosylation and Glycoproteins Research
- Cell Adhesion Molecules Research
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Immune cells in cancer
University Hospital of Basel
2016-2025
University of Basel
2009-2025
Semmelweis University
2023
University of Bern
2020
Taipei Institute of Pathology
2019
Hospital Base
2019
University of Zurich
2016
University of Vermont
2007
Psychiatry Baselland
2006
Heidelberg University
2005
Targeted drug delivery with antibody-drug conjugates such as the HER2-directed ado-trastuzumab emtansine (T-DM1) has emerged a powerful strategy for cancer therapy. We show that T-DM1 is particularly effective in eliciting antitumor immunity patients early breast (WSG-ADAPT trial) and HER2-expressing orthotopic tumor model. In latter, despite primary resistance to immunotherapy, combined treatment anti-CTLA-4/PD-1 (cytotoxic T lymphocyte-associated protein-4/programmed cell death protein-1)...
Macrophages block tumors' spread Tumors constantly communicate with their surrounding tissue and the immune system. One way tumors likely do this is by secreting extracellular vesicles (tEVs), which can carry bits of tumor to distant sites in body. Pucci et al. tracked tEVs tumor-bearing mice people studied how they affect cancer progression. They found that disseminate through lymph nearby nodes, where a specialized population macrophages largely any further travel. This barrier breaks...
Abstract Tumor associated inflammation predicts response to immune checkpoint blockade in human melanoma. Current theories on regulation of center anti-tumor T cell responses. Here we show that tumor B cells are vital melanoma inflammation. Human express pro- and anti-inflammatory factors differentiate into plasmablast-like when exposed autologous secretomes vitro. This phenotype can be reconciled melanomas where also cell-recruiting chemokines CCL3, CCL4, CCL5. Depletion patients by...
Immune checkpoint blockade (ICB) has substantially improved the prognosis of patients with cancer, but majority experiences limited benefit, supporting need for new therapeutic approaches. Up-regulation sialic acid–containing glycans, termed hypersialylation, is a common feature cancer-associated glycosylation, driving disease progression and immune escape through engagement Siglec receptors on tumor-infiltrating cells. Here, we show that tumor sialylation correlates distinct states reduced...
Myocardial fibrosis critically contributes to cardiac dysfunction in inflammatory dilated cardiomyopathy (iDCM). Activation of transforming growth factor-β (TGF-β) signalling is a key-step promoting tissue remodelling and iDCM. Downstream mechanisms controlling these processes, remain elusive.Experimental autoimmune myocarditis (EAM) was induced BALB/c mice with heart-specific antigen adjuvant. Using heart-inflammatory precursors, as well mouse human fibroblasts, we demonstrated rapid...
There is increasing evidence that tertiary lymphoid structures (TLS) control not only local adaptive B cell responses at melanoma tumor sites but also the cellular composition and function of other immune cells. In human melanoma, however, a comprehensive analysis TLS phenotypes, density spatial distribution different disease stages lacking. Here we used 7-color multiplex immunostaining whole tissue sections from 103 samples to characterize phenotypes along expression established...
Mitotic figure (MF) counting is important in the evaluation of many tumor types. Inadequate fixation, crush artefacts, presence apoptoses, or rarity MFs a given lesion can make determination mitotic index very time-consuming even impossible task, especially for novices. We evaluated potential two commercially available markers MPM-2 and Phospho-Histone H3 Ser28 (PHH3) improving counting. Formalin-fixed tissue 1 lymphoma, 19 epithelial, 25 mesenchymal, 10 melanocytic tumors was...
Abstract Multiple sclerosis (MS) is an inflammatory and possibly autoimmune mediated demyelinating disease of the CNS. Autoimmunity within CNS may be triggered by dysfunction peripheral immune tolerance mechanisms via changes in homeostatic composition T cells. We have assessed release naive lymphocytes from thymus patients with relapsing remitting MS (RRMS) to identify alterations equilibrium cell compartment. Thymic production was estimated measuring TCR excision circles (TRECs) as a...
Treatment of patients with advanced medullary thyroid carcinoma (MTC) is still a challenge. For more than 2 decades, it has been known that the cholecystokinin receptor promising target for treatment MTC radiolabeled minigastrin analogs. Unfortunately, kidney toxicity precluded their therapeutic application so far. In 6 consecutive patients, we evaluated 3-dimensional dosimetry whether improved analog <sup>177</sup>Lu-DOTA-(d-Glu)<sub>6</sub>-Ala-Tyr-Gly-Trp-Nle-Asp-PheNH<sub>2</sub>...
Adoptive cell therapy (ACT) with tumor-infiltrating lymphocytes (TIL) is effective in patients melanoma, although long-term responses seem restricted who have complete remissions. Many develop secondary resistance to TIL-ACT but the involved mechanisms are unclear. In this study, we describe a case of possibly due intratumoral heterogeneity and selection resistant tumor clone by transferred T cells. To best our knowledge, first clonal pre-existing nondominant clone; report demonstrates...
Abstract Pronounced forward flexion of the trunk, often termed camptocormia, is a typical symptom patients with Parkinson's disease. In 4 parkinsonian paraspinal muscles were studied by electromyography (EMG) and axial computerized tomography (CT) or magnetic resonance imaging (MRI) scans muscle biopsy. EMG lumbar thoracic paravertebral showed abundant fibrillations, positive sharp waves, bizarre high‐frequency discharges. Spinal CT MRI revealed variable degrees atrophy fatty replacement...
Abstract Mood, emotion and cognition are modulated by serotonergic neurotransmission, while the physiological function of synapses depends on serotonin reuptake, which is mediated transporter (5‐HTT). Allelic variation 5‐HTT expression in humans caused a functional gene‐promoter polymorphism with two predominant variant alleles, associated variations anxiety measures as previously reported. Here we report that administration dexamethasone, potent glucocorticosteroid hormone, results an...
This Internet-based quiz (http://kathrin.unibas.ch/polyp/) tested the diagnostic variability of 168 pathologists in diagnosis 20 colorectal polyps on 3 representative images, including hyperplastic (HPs), traditional serrated adenomas (TSAs), sessile (SSAs), and tubulovillous (TVAs). Interobserver for each lesions was significant most pronounced SSAs. Correct answers were independent participant's experience with TVAs, HPs, TSAs. Participants gastrointestinal subspecialty training those who...
Intratumoral heterogeneity and clonal evolution are pivotal in the progression metastasis of melanoma. However, when combined with variable tumor cellularity, intratumoral limits sensitivity accuracy uncovering a cancer's evolution. In this study, we fluorescence-activated cell sorting (FACS) whole-exome sequencing (WES) to investigate composition evolutionary patterns seven melanoma biopsies obtained from three patients, each having both primary site metastatic samples. We employed...
Urinary cytology is limited by high interobserver variability in the evaluation of cells with little atypia. We set up an online quiz on urinary and tested performance 246 international participants. The consisted still images 42 specimens equivocal morphologic features 10 control cases unequivocal cytologic diagnosis. nature 292 had been verified multitarget fluorescence situ hybridization addition to information obtained cystoscopy, clinical follow up, and/or histologic examination....
Urinary cytology is limited by high interobserver variability in the evaluation of cells with little atypia. We set up an online quiz on urinary and tested performance 246 international participants. The consisted still images 42 specimens equivocal morphologic features 10 control cases unequivocal cytologic diagnosis. nature 292 had been verified multitarget fluorescence situ hybridization addition to information obtained cystoscopy, clinical follow-up, and/or histologic examination....
Abstract While coronavirus disease 2019 (COVID‐19) primarily affects the respiratory tract, pathophysiological changes of cardiovascular system remain to be elucidated. We performed a retrospective cardiopathological analysis heart and vasculature from 23 autopsies COVID‐19 patients, comparing findings with control tissue. Myocardium patients was categorised into severe acute syndrome 2 (SARS‐CoV‐2) positive ( n = 14) or negative 9) based on presence viral RNA as determined by reverse...
Tumor cell fraction (TCF) estimation is a common clinical task with well-established large interobserver variability. It thus provides an ideal test bed to evaluate potential impacts of employing tumor computer-aided diagnostic (TCFCAD) tool support pathologists' evaluation. During National Slide Seminar event, pathologists (n = 69) were asked visually estimate TCF in 10 regions interest (ROIs) from hematoxylin and eosin colorectal cancer images intentionally curated for diverse tissue...
BackgroundExtracellular traps formed by neutrophils (NETs) and eosinophils (EETs) have been described in coronary thrombi, contributing to thrombus stability. A key mechanism during NET formation is histone modification the enzyme PAD4. Citrullinated histones, product of PAD4 activity, are often attributed neutrophils. Eosinophils also express high levels PAD4.ObjectivesWe aimed explore contribution EET formation.MethodsWe performed immunohistological analyses on including a large, intact,...
The presence and distribution of mitotic figures is an important discriminatory parameter in the assessment melanocytic lesions. We evaluated number 353 randomly collected nevi various subtypes by hematoxylin eosin (H&E) staining immunohistochemically with 2 markers Phospho-Histone H3 Ser28 (PHH3) MPM2. At least 1 figure was present 19.5%, 31.3%, 42.8% H&E-, PHH3-, MPM2-stained lesions, respectively. In common compound nevi, mean dermal mitoses amounted to 0.024/mm surface area H&E (PHH3:...