A5020161524- Ionic liquids properties and applications
- Computational Drug Discovery Methods
- Synthesis and biological activity
- Silk-based biomaterials and applications
- HER2/EGFR in Cancer Research
- Extracellular vesicles in disease
- Fibroblast Growth Factor Research
- Advanced biosensing and bioanalysis techniques
- Tribology and Wear Analysis
- Proteoglycans and glycosaminoglycans research
- Integrated Circuits and Semiconductor Failure Analysis
- Bee Products Chemical Analysis
- Signaling Pathways in Disease
- Microscopic Colitis
- Skin Protection and Aging
- Cellular Mechanics and Interactions
- Synthesis of Tetrazole Derivatives
- Innovative Microfluidic and Catalytic Techniques Innovation
- Phytochemistry and Bioactivity Studies
- Clostridium difficile and Clostridium perfringens research
- Viral Infectious Diseases and Gene Expression in Insects
- Galectins and Cancer Biology
- Membrane Separation and Gas Transport
- Adhesion, Friction, and Surface Interactions
- Enzyme Production and Characterization
Warsaw University of Technology
2011-2024
Institute of Biochemistry and Biophysics, Polish Academy of Sciences
2005-2014
Mid Essex Hospital Services NHS Trust
2009
Broomfield Hospital
2009
Polish Academy of Sciences
2007
Abstract A simple and efficient procedure for the synthesis of new optically active imidazolium triazolium ionic liquids in a three step reaction sequence is described. In first step, ring opening 1,2‐butylene oxide by imidazole or 1,2,4‐triazole resulted formation N ‐2‐hydroxybutylimidazole ‐2‐hydroxybutyl‐1,2,4‐triazole, respectively. second racemic mixtures secondary alcohols were resolved with good enantioselectivity lipase‐catalyzed transesterification enol esters (vinyl acetate...
Extracellular vesicles (EVs) are nanoparticles containing various bioactive cargos-e.g., proteins, RNAs, and lipids-that released into the environment by all cell types. They involved in, amongst other functions, intercellular communication. This article presents studies on EVs produced probiotic yeast Saccharomyces boulardii CNCM I-745. The size distribution concentration of in liquid culture were estimated. Moreover, S. tested for their cytotoxicity against three model human intestinal...
Atomic force microscopy (AFM) and fluorescence was applied to determine the influence of anti-aging peptides on morphology mechanical properties keratinocytes. Immortalized human keratinocytes (HaCaT) were treated with two bioactive peptides: Acetyl Tetrapeptide-2 Hexapeptide-50 (Lipotec). The AFM measurement keratinocyte stiffness carried after 48 h exposure at an indentation depth 200 nm. analysis showed increase cell for cells (P1) in concentration range. (P2) 0.05 µg/ml also increased...
Immunosensors based on gold electrodes (electrochemical) or discs (optical) modified with 1,6-hexanedithiol, nanorods and Anti-His (C-term) monoclonal antibody F(ab') fragment are described. The antigen detected by the sensing platform is a recombinant histidine-tagged silk proteinase inhibitor (rSPI2-His(6)). Electrochemical impedance spectroscopy (EIS) surface plasmon resonance (SPR) techniques were used as methods for detection of antigen. This approach allows to detect protein in...
Racemic 1-(β-hydroxypropyl)azoles were prepared by solvent-free direct regioselective ring opening of 1,2-propylene oxide with imidazole or 1,2,4-triazole. Lipase-catalyzed transesterification alcohols vinyl acetate resulted in kinetic enantiomers resolution. Separated (S)-enantiomers (+)-1-(1H-imidazol-1-yl)propan-2-ol and (+)-1-(1H-1,2,4-triazol-1-yl)propan-2-ol quaternized alkyl bromides iodides, yielding novel optically active ionic liquids. salts tested against a wide range microorganisms.
Protein kinase CK2 has been considered as an attractive drug target for anti-cancer therapy. The synthesis of
This work was focused on biodegradation with Escherichia coli bacteria studies of PSF-PUR blend semipermeable hollow fiber membranes that possibly can undergo a partial degradation process. Hollow were obtained from polysulfone (PSF) and polyurethane (PUR) containing ester bonds in the polymer chain various weight ratios using two solvents: N,N-Dimethylmethanamide (DMF) or N-Methylpyrrolidone (NMP). The underwent process tested for changes ultrafiltration coefficient (UFC), retention cut-off...
An effective therapy for advanced melanoma, a skin cancer with the highest mortality, has not yet been developed. The endocannabinoid system is considered to be an attractive target treatment. use of endocannabinoids, such as anandamide (AEA), much greater than palliative agent. Thus, we checked its influence on various signaling pathways in melanoma cells. Our investigation was performed four commercial cell lines derived from different progression stages (radial WM35 and vertical WM115...
W pracy przedstawiono przegląd danych literaturowych dotyczących budowy i wlaściwości kwasu hialuronowego (HA). Wskazano miejsca wystepowania tego związku w organizmie czlowieka oraz pelnione przez niego funkcje. Omowiono sposoby otrzymywania z materialu zwierzecego przy wykorzystaniu procesow biotechnologicznych. cechy dodatnie ujemne preparatow otrzymanych dwoma ww. sposobami. cześci artykulu dotyczącej wytwarzania bakterie zwrocono szczegolną uwage na modyfikacje genomu bakterii...
Abstract Violacein is a natural indole-derived purple pigment of microbial origin that has attracted attention for its remarkable biological properties. Due to poor solubility in aqueous media, most studies this use extracts the compound obtained with common solvents. also transported bacterial extracellular vesicles (EVs) and transferred via type carrier remains stable an environment. This paper first present in-depth study Janthinobacterium lividum EVs as violacein carriers. J. were...
Exogenous proteinase inhibitors are valuable and economically interesting protective biotechnological tools. We examined whether small when fused to a selected target protein can protect the from proteolytic degradation without simultaneously affecting function activity of domain. Two were studied: Kazal-type silk inhibitor (SPI2) Galleria mellonella, Cucurbita maxima trypsin I (CMTI I). Both serine proteinases, proteins with compact structure stabilized by network disulfide bridges,...
Scaffold-based analogs of cinnamic acid benzyl amide (CABA) exhibit pleiotropic effects in cancer cells, and their exact molecular mechanism action is under investigation. The present study part our systemic analysis interactions CABA with targets. These compounds were shown to inhibit Janus kinase 2 (JAK2)/signal transducer activator transcription 3 (STAT3) JAK2/signal 5 (STAT5) signaling thus are attractive scaffolds for anticancer drug design. To identify the potential mechanisms this...