A5091028522- Cardiomyopathy and Myosin Studies
- Muscle Physiology and Disorders
- Cellular Mechanics and Interactions
- Genetic Neurodegenerative Diseases
- RNA Research and Splicing
- Cellular transport and secretion
- Neurogenetic and Muscular Disorders Research
- Microtubule and mitosis dynamics
- Nuclear Structure and Function
- Heat shock proteins research
- Cancer Research and Treatments
- Ion channel regulation and function
- Erythrocyte Function and Pathophysiology
- Cardiovascular Effects of Exercise
- Mitochondrial Function and Pathology
- Signaling Pathways in Disease
- Protein Kinase Regulation and GTPase Signaling
- RNA modifications and cancer
- Renal and related cancers
- Sperm and Testicular Function
- Cell Adhesion Molecules Research
- Reproductive Biology and Fertility
- Cancer, Hypoxia, and Metabolism
- Congenital heart defects research
- Fungal and yeast genetics research
Polish Academy of Sciences
2014-2024
Instytut Biologii Doświadczalnej im. Marcelego Nenckiego
2015-2024
Rzeszów University
2021
National Heart Lung and Blood Institute
1994-1999
National Institutes of Health
1994-1999
Office of the Director
1996
National Institute of Diabetes and Digestive and Kidney Diseases
1996
Endocannabinoid signaling has been implicated in modulating insulin release from β cells of the endocrine pancreas. Cells express CB1 cannabinoid receptors (CB1Rs), and enzymatic machinery regulating anandamide 2-arachidonoylglycerol bioavailability. However, molecular cascade coupling agonist-induced receptor activation to remains unknown. By combining pharmacology genetic tools INS-1E vivo, we show that CB1R by endocannabinoids (anandamide 2-arachidonoylglycerol) or synthetic agonists...
A deficit of exogenous arginine affects growth and viability numerous cancer cells. Although deprivation-based strategy is currently undergoing clinical trials, molecular mechanisms tumor cells' response to deprivation are not yet elucidated. We have examined effects starvation on cell motility, adhesion invasiveness as well actin cytoskeleton organization human glioblastoma observed for the first time that arginine, but lysine, affected morphology, significantly inhibited their motility...
Myofibrillar myopathy (MFM) is a group of inherited muscular disorders characterized by myofibrils dissolution and abnormal accumulation degradation products. So far causative mutations have been identified in nine genes encoding Z-disk proteins, including αB-crystallin (CRYAB), small heat shock protein (also called HSPB5). Here, we report case study 63-year-old Polish female with progressive lower limb weakness muscle biopsy suggesting myofibrillar myopathy, extra-muscular multisystemic...
Limb girdle muscular dystrophies (LGMD) are a group of heterogeneous hereditary myopathies with similar clinical symptoms. Disease onset and progression highly variable, an elusive genetic background, around 50% cases lacking molecular diagnosis. Whole exome sequencing (WES) was performed in 73 patients clinically diagnosed LGMD. A filtering strategy aimed at identification variants related to the disease included integrative analysis WES data human phenotype ontology (HPO) terms, genes...
Crataegus contains numerous health-promoting compounds that are also proposed to have anti-cancer properties. Herein, we aimed at a contemporaneous evaluation of the effects polyphenol-rich extracts berries, leaves, and flowers six species on viability invasive potential highly aggressive human glioblastoma U87MG cell line. The treatment with evoked cytotoxic effects, strongest in berry extracts. All not only promoted apoptosis-related cleavage poly (ADP-ribose) polymerase 1 (PARP1) but...
Ruk/CIN85 is a mammalian adaptor molecule with three SH3 domains. Using its domains can cluster multiple proteins and protein complexes, and, consequently, facilitates organisation of elaborate interaction networks diverse regulatory roles. Previous research linked the regulation vesicle-mediated transport cancer cell invasiveness. Despite recent findings, precise molecular functions in these processes remain largely elusive further hampered by lack complete lists partner proteins. In...
BAG3 belongs to BAG family of molecular chaperone regulators interacting with HSP70 and anti-apoptotic protein Bcl-2. It is ubiquitously expressed strong expression in skeletal cardiac muscle, involved a panoply cellular processes. Mutations aberrations its cause fulminant myopathies, presenting progressive limb axial muscle weakness, respiratory insufficiency neuropathy. Herein, we report sporadic case 15-years old girl symptoms myopathy, demyelinating polyneuropathy asymptomatic long QT...
Myosin VI (MVI), the only known myosin that walks towards minus end of actin filaments, is involved in several processes such as endocytosis, cell migration, and cytokinesis. It may act a transporting motor or protein engaged cytoskeleton remodelling via its binding partners, interacting with C-terminal globular tail domain. By means pull-down technique mass spectrometry, we identified Dock7 (dedicator cytokinesis 7) potential novel MVI-binding partner neurosecretory PC12 cells. Dock7,...
Myosin VI (MVI) is a unique unconventional motor moving backwards on actin filaments. In non-muscle cells, it involved in cell migration, endocytosis and intracellular trafficking, cytoskeleton dynamics, possibly gene transcription. An important role for MVI striated muscle functioning was suggested report showing that point mutation (H236R) within the associated with cardiomyopathy (Mohiddin et al., J Med Genet 41:309–314, 2004). Here, we have addressed function by examining its expression...
We have previously shown that unconventional myosin VI (MVI), a unique actin-based motor protein, shuttles between the cytoplasm and nucleus in neurosecretory PC12 cells stimulation-dependent manner interacts with numerous proteins involved nuclear processes. Among identified potential MVI partners was nucleolin, major nucleolar protein implicated rRNA processing ribosome assembly. Several other such as fibrillarin, UBF (upstream binding factor), B23 (also termed nucleophosmin) been to...
Myosin VI (MVI) is the only known myosin walking towards minus end of actin filaments and believed to play distinct role(s) than other myosins. We addressed a role this unique motor in secretory PC12 cells, derived from rat adrenal medulla pheochromocytoma using cell lines with reduced MVI synthesis (produced by means siRNA). Decrease expression caused severe changes size morphology, profound defects cytoskeleton organization Golgi structure. Also, significant inhibition migration as well...
During spermiogenesis in mammals, actin filaments and a variety of actin-binding proteins are involved the formation function highly specialized testis-specific structures. Actin-based motor proteins, such as myosin Va VIIa, play key role this complex process spermatid transformation into mature sperm. We have previously demonstrated that VI (MYO6) is also expressed mouse testes. It present actin-rich structures important for development, including one earliest events spermiogenesis-acrosome...
The important role of unconventional myosin VI (MVI) in skeletal and cardiac muscle has been recently postulated (Karolczak et al. Histochem Cell Biol 139:873–885, 2013). Here, we addressed for the first time a this unique motor myogenic cells as well during their differentiation into myotubes. During myoblast differentiation, isoform expression pattern MVI its subcellular localization underwent changes. In undifferentiated myoblasts, MVI-stained puncti were seen throughout cytoplasm close...