A5083988482- Cancer, Hypoxia, and Metabolism
- Cancer Cells and Metastasis
- Cancer Research and Treatments
- 3D Printing in Biomedical Research
- Radiopharmaceutical Chemistry and Applications
- Glioma Diagnosis and Treatment
- Angiogenesis and VEGF in Cancer
- Boron Compounds in Chemistry
- Cell Adhesion Molecules Research
- Lung Cancer Treatments and Mutations
- Virus-based gene therapy research
- Cellular Mechanics and Interactions
- Ovarian cancer diagnosis and treatment
- Inflammatory mediators and NSAID effects
- Immune cells in cancer
- Immunotherapy and Immune Responses
- Radiation Therapy and Dosimetry
- Cancer Genomics and Diagnostics
- Immune Cell Function and Interaction
- Chemical Reactions and Isotopes
- Mathematical Biology Tumor Growth
- T-cell and B-cell Immunology
- Amino Acid Enzymes and Metabolism
- Prostate Cancer Treatment and Research
- Melanoma and MAPK Pathways
TU Dresden
2016-2025
Helmholtz-Zentrum Dresden-Rossendorf
2016-2025
University Hospital Carl Gustav Carus
2016-2025
OncoRay
2015-2025
National Center for Tumor Diseases
2017-2025
German Cancer Research Center
2019-2025
Heidelberg University
2019-2025
University Hospital Heidelberg
2019-2025
CRUK/MRC Oxford Institute for Radiation Oncology
2016-2018
University of Oxford
2016-2017
Invasion and dissemination of well-differentiated carcinomas are often associated with loss epithelial differentiation gain mesenchyme-like capabilities the tumor cells at invasive front. However, when comparing central areas primary colorectal corresponding metastases, we again found same differentiated growth patterns. These characteristic phenotypic changes were distinct expression patterns β-catenin, main oncogenic protein in carcinomas, E-cadherin. Nuclear β-catenin was dedifferentiated...
High-fat (HF)-diet rodent models have contributed significantly to the analysis of pathophysiology insulin resistance syndrome, but their phenotype varies distinctly between different studies. Here, we systematically compared metabolic and molecular effects HF with varying fatty acid compositions. Male Wistar rats were fed diets (42% energy; fat sources: HF-L – lard; HF-O olive oil; HF-C coconut fat; HF-F fish oil). Weight, food intake, whole-body tolerance plasma parameters glucose lipid...
Abstract High concentrations of lactic acid (LA) are found under various pathophysiological conditions and accompanied by an acidification the environment. To study impact LA on TNF secretion, human LPS-stimulated monocytes were cultured with or without corresponding pH control. secretion was significantly suppressed low (≤10 mM), whereas only strong had a similar effect. This result confirmed in coculture model multicellular tumor spheroids. Blocking synthesis tumor-derived lactate oxamic...
Abstract The prognosis of patients suffering from glioblastoma (GBM) is dismal despite multimodal therapy. Although chemotherapy with temozolomide may contain tumor growth for some months, invariable recurrence suggests that cancer stem cells (CSC) maintaining these tumors persist. We have therefore investigated the effect on CD133+ and CD133− GBM CSC lines. differentiated constituting bulk all were resistant to cytotoxic effects substance, induced a dose- time-dependent decline cell...
Radiotherapy is a curative treatment option in prostate cancer. Nevertheless, patients with high-risk cancer are prone to relapse. Identification of the predictive biomarkers and molecular mechanisms radioresistance bears promise improve therapies. In this study, we show that aldehyde dehydrogenase (ALDH) activity indicative radioresistant progenitor cells an enhanced DNA repair capacity activation epithelial-mesenchymal transition (EMT). Gene expression profiling cells, their derivatives,...
Abstract Tumour hypoxia renders cancer cells resistant to therapy, resulting in markedly worse clinical outcomes. To find candidate compounds that reduce tumours, we conduct a high-throughput screen for oxygen consumption rate (OCR) reduction and identify number of drugs with this property. For study focus on the anti-malarial, atovaquone. Atovaquone rapidly decreases OCR by more than 80% wide range cell lines at pharmacological concentrations. In addition, atovaquone eradicates FaDu, HCT116...
Radiotherapy is one of the curative treatment options for localized prostate cancer (PCa).The potential radiotherapy mediated by irradiation-induced oxidative stress and DNA damage in tumor cells.However, PCa radiocurability can be impeded resistance mechanisms normal tissue toxicity.Metabolic reprogramming major hallmarks progression therapy resistance.Specific metabolic features might serve as therapeutic targets radiosensitization biomarkers Ivyspring International Publisheridentifying...
Abstract CD68, the human homologue of macrosialin, is commonly regarded as a selective marker for monocytes and macrophages. Its expression thought to be regulated by macrophage‐specific promoter. However, several immunohistochemical studies have indicated that CD68 antibodies also react with other haematopoietic non‐haematopoietic cell types. We investigated in various primary cells carcinoma lines using immunohistochemistry, flow cytometry, Western blot analysis qRT‐PCR. Weak but...
Cell-based assays are more complex than cell-free test systems but still reflect a highly artificial cellular environment.Incorporation of organotypic 3-dimensional (3-D) culture into mainstream drug development processes is increasingly discussed severely limited by methodological requirements.The objective this study was to explore panel standard provide an easy-handling, standardized protocol for rapid routine analysis cell survival in multicellular tumor spheroid-based antitumor...
The development of vessel-like structures in vitro to mimic as well realize the possibility tissue-engineered small vascular networks presents a major challenge cell biologists and biotechnologists. We aimed establish three-dimensional (3-D) culture system with an endothelial network that does not require artificial substrates or ECM compounds. By using human skin fibroblasts cells (ECs) from umbilical vein (HUVECs) diverse spheroid coculture strategies, we verified fibroblast support...
Despite recent advances in understanding of the molecular pathogenesis and improvement treatment techniques, locally advanced head neck squamous cell carcinoma (HNSCC) remains associated with an unfavorable prognosis. Compelling evidence suggests that cancer stem cells (CSC) may cause tumor recurrence if they are not eradicated by current therapies as radiotherapy or radio-chemotherapy. Recent vitro studies have demonstrated CSCs be protected from treatment-induced death multiple intrinsic...