A5079095632- Glioma Diagnosis and Treatment
- Cancer Cells and Metastasis
- Neurogenesis and neuroplasticity mechanisms
- Cerebrovascular and Carotid Artery Diseases
- Amyotrophic Lateral Sclerosis Research
- Traumatic Brain Injury and Neurovascular Disturbances
- Neurogenetic and Muscular Disorders Research
- Acute Ischemic Stroke Management
- TGF-β signaling in diseases
- Cancer, Hypoxia, and Metabolism
- Advanced MRI Techniques and Applications
- 3D Printing in Biomedical Research
- Parkinson's Disease Mechanisms and Treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Cerebral Venous Sinus Thrombosis
- Pluripotent Stem Cells Research
- MicroRNA in disease regulation
- Cytokine Signaling Pathways and Interactions
- Brain Metastases and Treatment
- Genetic Neurodegenerative Diseases
- Nerve injury and regeneration
- Neurological diseases and metabolism
- Ultrasound and Hyperthermia Applications
- Neuroblastoma Research and Treatments
- Metabolism, Diabetes, and Cancer
University Hospital Regensburg
2015-2025
University of Regensburg
2007-2025
Paracelsus Medical University
2010-2021
Trinity College Dublin
2020
Klinik und Poliklinik für Neurologie
1989-2019
University Hospital Münster
2015
Boston Collaborative Drug Surveillance Program
2015
Boston University
2015
University Hospital of Basel
2015
University of Basel
2015
Abstract Although glioblastomas show the same histologic phenotype, biological hallmarks such as growth and differentiation properties vary considerably between individual cases. To investigate whether different subtypes of might originate from cells origin, we cultured tumor 22 under medium conditions favoring neural cancer stem (CSC). Secondary glioblastoma (n = 7)–derived did not any in used, suggesting absence cell–like cells. In contrast, 11/15 primary contained a significant CD133+...
Abstract Progress in the field of neurogenesis is currently limited by lack tools enabling fast and quantitative analysis adult brain. Doublecortin (DCX) has recently been used as a marker for neurogenesis. However, it was not clear whether DCX could be to assess modulations occurring rate mammalian central nervous system following lesioning or stimulatory factors. Using two paradigms increasing levels (physical activity epileptic seizures), we demonstrate that quantification DCX‐expressing...
Article abstract-To detect morphologic abnormalities in Parkinson9s disease (PD), we examined 30 patients with PD and age- sex-matched nonparkisonian controls by transcranial color-coded real-time sonography (TCCS). In 12 severely affected patients, the echogenicity of substantia nigra was distinctly increased. remaining 18 all controls, poorly visualized or nondetectable TCCS. The degree hyperechogenicity substania closely correlated severity duration (p < 0.001). increased notably...
Abstract The prognosis of patients suffering from glioblastoma (GBM) is dismal despite multimodal therapy. Although chemotherapy with temozolomide may contain tumor growth for some months, invariable recurrence suggests that cancer stem cells (CSC) maintaining these tumors persist. We have therefore investigated the effect on CD133+ and CD133− GBM CSC lines. differentiated constituting bulk all were resistant to cytotoxic effects substance, induced a dose- time-dependent decline cell...
Fibromyalgia (FM), among other chronic pain syndromes, such as tension type headache and atypical face pain, is classified a so-called dysfunctional syndrome. Patients with fibromyalgia suffer from widespread, “deep” muscle often report concomitant depressive episodes, fatigue cognitive deficits. Clear evidence for structural abnormalities within the muscles or soft tissue of patients lacking. There growing that clinical in has to be understood terms pathological activity central structures...
Cerebral amyloid angiopathy (CAA) is a degenerative disorder characterized by amyloid-β (Aβ) deposition in the blood-brain barrier (BBB). CAA contributes to injuries of neurovasculature including lobar hemorrhages, cortical microbleeds, ischemia, and superficial hemosiderosis. We postulate that pathology partially due Aβ compromising BBB.We 19 patients with acute stroke "probable CAA" for neurovascular based on MRI clinical findings. Also, we studied effect expression tight junction proteins...
Transforming growth factor-beta2 (TGF-β2) is known to suppress the immune response cancer cells and plays a pivotal role in tumor progression by regulating key mechanisms including proliferation, metastasis, angiogenesis. For targeted protein suppression TGF-β2-specific antisense oligodeoxynucleotide AP 12009 was developed. In vitro experiments have been performed prove specificity efficacy of TGF-β2 inhibitor employing patient-derived malignant glioma as well peripheral blood mononuclear...
IV thrombolysis represents the most effective acute stroke therapy. However, it is almost exclusively performed in centers and not available community areas. The Telemedical Pilot Project for Integrative Stroke Care (TEMPiS) was started February 2003. Twelve hospitals with no or very limited experience two were connected via a network providing online neurologic examination transfer of neuroradiologic scans. Following recently published preliminary results on phase safety telethrombolysis,...
Abstract As human life expectancy has improved rapidly in industrialized societies, age-related cognitive impairment presents an increasing challenge. Targeting histopathological processes that correlate with declines, such as neuroinflammation, low levels of neurogenesis, disrupted blood–brain barrier and altered neuronal activity, might lead to structural functional rejuvenation the aged brain. Here we show a 6-week treatment young (4 months) old (20 rats montelukast, marketed...
Transforming growth factor (TGF)-β1 has multiple functions in the adult central nervous system (CNS). It modulates inflammatory responses CNS and controls proliferation of microglia astrocytes. In diseased brain, TGF-β1 expression is upregulated and, depending on cellular context, its activity can be beneficial or detrimental regarding regeneration. We focus role neural stem cell biology neurogenesis. progenitor cultures after intracerebroventricular infusion, induced a long-lasting...
Myeloid-derived suppressor cells (MDSCs) comprise a heterogeneous population of myeloid that are significantly expanded in cancer patients and associated with tumor progression.Multicolor flow cytometry was used to study the frequency, phenotype, function MDSCs peripheral blood freshly resected tumors 52 participants primary glioblastoma (GBM).The frequency CD14(high)CD15(pos) monocytic CD14(low)CD15(pos) granulocytic higher GBM compared healthy donors. The majority consisted...
Serial magnetic resonance imaging (MRI) was performed to investigate the temporal and spatial relationship between biphasic nature of blood-brain barrier (BBB) opening and, in parallel, edema formation after ischemia-reperfusion (I/R) injury rats. T(2)-weighted combined with T(2)-relaxometry, mainly for assessment, at 1 h ischemia, reperfusion, 4, 24 48 reperfusion. T(1)-weighted before gadolinium contrast last three time points assess BBB integrity. The course a significant reduction...
Stroke Unit care improves stroke prognosis and is recommended for all patients with stroke. In rural areas, population-wide implementation of Units challenging. Therefore, the TeleMedical Project integrative Care (TEMPiS) was established in 2003 as a TeleStroke network to overcome this barrier Southeast Bavaria/Germany. Evaluation its between 2005 had revealed improved process quality clinical outcomes compared matched hospitals without Units. Data on sustainability these effects are...
Abstract Members of the transforming growth factor ( TGF )‐β family govern a wide range mechanisms in brain development and adult, particular neuronal/glial differentiation survival, but also cell cycle regulation neural stem maintenance. This clearly created some discrepancies field with studies favouring neuronal differentiation/survival progenitors others exit quiescence/maintenance. Here, we provide unifying hypothesis claiming that through its progenitor NPC ) proliferation, ‐β...
Idiopathic cervical dystonia (ICD), the most common adult-onset focal dystonia, is clinically characterized by involuntary uni- or bilateral contractions of head and neck musculature. One crucial criterion for diagnosis ICD normal results on MRI brain.To test hypothesis whether subtle brain structure changes occur in ICD.Using voxel-based morphometry, authors compared 10 patients with that healthy sex- age-matched controls using high-resolution MRI.Patients showed an increase gray matter...
BACKGROUND Resistance to chemotherapeutic agents and poor blood-brain barrier penetration are major limitations in the treatment of malignant glioma. To improve drug delivery across barrier, authors used doxorubicin as liposomal encapsulated formulation (Caelyx, Scheringh-Plough, Munich, Germany) therapy recurrent METHODS Fifteen patients with high-grade gliomas were included study. Of these, 13 could be evaluated, including 6 glioblastoma, 1 patient gliosarcoma anaplastic astrocytoma. The...
Background and Purpose— Stroke unit treatment is effective in reducing death dependency after stroke but not available many, particularly rural, areas. The implementation of a network with telemedicine support was associated improved outcome at 3 months. We report follow-up results 12 30 months acute stroke. Methods— Telemedical Project for Integrative Care (TEMPiS) consists the set-up specialized local wards, continuous medical education, telemedical consultation patients by 2 centers. In...
Abstract Progress in the field of neurogenesis is limited by lack animal models allowing direct detection and analysis living cells participating neurogenesis. We engineered a transgenic mouse model that expresses fluorescent reporter proteins enhanced green protein or Discoma sp. reef coral red under control doublecortin (DCX) promoter, gene specifically transiently active neuronal precursors young neurons. The expression correlated with endogenous DCX protein, developmental adult...
Abstract Objective Hereditary spastic paraplegias (HSPs) comprise a heterogeneous group of neurodegenerative disorders resulting in progressive spasticity the lower limbs. One form autosomal recessive hereditary paraplegia (ARHSP) with thin corpus callosum (TCC) was linked to chromosomal region 15q13‐21 (SPG11) and associated mutations spatacsin gene. We assessed long‐term course mutational spectrum ‐associated ARHSP TCC. Methods Neurological examination, cerebral magnetic resonance imaging...
Lactate formation in highly proliferative tumors such as malignant gliomas is associated with poor survival and contributes to the suppression of local immunity. Here, we report that diclofenac used at nontoxic concentrations significantly decreased lactate production murine glioma cells inhibited expression dehydrogenase-A vitro. reduction was accompanied by a dose-dependent inhibition cell growth cycle arrest G2/M checkpoint. In presence diclofenac, bone marrow-derived dendritic (DCs)...
Cellular proliferation, differentiation, integration, and survival within the adult neural stem cell niche are altered under pathological conditions, but molecular cues regulating biology of this mostly unknown. We examined hippocampal in a transgenic rat model Huntington disease. In model, progressive cognitive deficits develop at age 9months, suggesting possible dysfunction. found disease-associated decline progenitor proliferation accompanied by an expansion pool 5-bromo-2-deoxyuridine...
An important phenomenon observed in glioma metabolism is increased aerobic glycolysis tumor cells, which generally referred to as the Warburg effect. Transforming growth factor (TGF)-beta2, we previously showed be induced by lactic acid, a key pathophysiological glioblastoma, leading invasion and severe local immunosuppression after proteolytic cleavage from its latency associated peptide. In this study tested hypothesis, that lactate regulates TGF-beta2 expression cell migration via...