The development of vessel-like structures in vitro to mimic as well realize the possibility tissue-engineered small vascular networks presents a major challenge cell biologists and biotechnologists. We aimed establish three-dimensional (3-D) culture system with an endothelial network that does not require artificial substrates or ECM compounds. By using human skin fibroblasts cells (ECs) from umbilical vein (HUVECs) diverse spheroid coculture strategies, we verified fibroblast support...

Objectives Technical complications are a known hazard in veno-venous extracorporeal membrane oxygenation (vvECMO). Identifying these and predictive factors indicating developing system-exchange was the goal of study. Methods Retrospective study on prospectively collected data technical including 265 adult patients (Regensburg ECMO Registry, 2009-2013) with acute respiratory failure treated vvECMO. Alterations blood flow resistance, gas transfer capability, hemolysis, coagulation hemostasis...

Venovenous (VV) and venoarterial (VA) extracorporeal membrane oxygenation (ECMO) are effective support modalities to treat critically ill patients. ECMO-associated hemolysis remains a serious complication. The aim was disclose similarities differences in VA- VV hemolysis. This is retrospective single-center analysis (January 2012 September 2018) including 1,063 adult consecutive patients (VA, n = 606; VV, 457). Severe (free plasma hemoglobin, fHb > 500 mg/l) during therapy occurred 4% 2%...

Bioline-coated polymethylpentene (PMP) membrane oxygenators (MO) are used for extracorporeal oxygenation (ECMO) to improve gas exchange in patients with severe acute respiratory distress syndrome (ARDS). However, some patients, long-term durability is limited due fibrous and cellular accumulations on the surface which can increase resistance blood flow diffusion path. These deposits of PMP MO after removal were studied scanning electron fluorescence microscopy techniques. Three 31 supported...

The aim of the study was to explore prevalence and risk factors for technical-induced hemolysis in adults supported with veno-venous extracorporeal membrane oxygenation (vvECMO) analyze effect hemolytic episodes on outcome. This a retrospective, single-center that included 318 adult patients (Regensburg ECMO Registry, 2009–2014) acute respiratory failure treated different modern miniaturized systems. Free plasma hemoglobin (fHb) used as indicator hemolysis. Throughout cumulative support...

Objective: Extracorporeal membrane oxygenation is a rescue therapy for patients with severe lung failure. Major complications caused by extracorporeal are bleeding, thrombosis, and hemolysis. The aim of this study was to compare the impact different systems on blood hemostasis in adults during veno-venous therapy. Design: Single center prospective randomized study. Setting: University Hospital Regensburg, Germany. Patients: Adult acute respiratory distress syndrome requiring Interventions:...

Thrombosis inside the membrane oxygenator (MO) is a critical complication during venovenous extracorporeal oxygenation (ECMO). The aim of this study was to prove if thrombotic clots manifest within MO when D-dimer levels are elevated over long-term period. Heparin-coated polymethylpentene MOs (n = 13) were exchanged due high plasma levels. Clot volume calculated using multidetector computed tomography (MDCT). Coagulation parameters and function analyzed before after exchange. Before...

Abstract Membrane oxygenator ( MO ) failure is a known hazard during venovenous extracorporeal membrane oxygenation (v‐v ECMO therapy. Knowledge about technical and performance details of different systems M aquet, R astatt, G ermany; edos, S tolberg, orin, odena, I taly) licensed for adults with acute lung might improve their handling. This retrospective study comprises 186 adult patients egensburg egistry) treated v‐v . Flow dynamic data were used to analyze the blood pumps, cannula types,...

Abstract Neutrophil extracellular traps (NETs) were detected in blood samples and cellular deposits of oxygenator membranes during extracorporeal membrane oxygenation (ECMO) therapy may be responsible for thrombogenesis. The aim was to evaluate the effect base material gas fiber (GF, polymethylpentene) heat exchange (HE) different antithrombogenic coatings on isolated granulocytes from healthy volunteers under static culture conditions. Contact with ECMO oxygenators (with surface coatings)...

Contact of blood with artificial surfaces triggers platelet activation. The aim was to compare kinetics after venovenous extracorporeal membrane oxygenation (V-V ECMO) start and system exchange in different etiologies acute lung failure. Platelet counts coagulation parameters were analyzed from adult patients long exchange-free (≥8 days) ECMO runs (n = 330) caused by bacterial 142), viral 76), or coronavirus disease 2019 (COVID-19) 112) pneumonia. A subpopulation requiring a long, the second...

Pressure‐induced dissociation of ribosomes has been considered a major reason for the inhibition protein biosynthesis and, hence, bacterial growth at high hydrostatic pressure [Jaenicke, R. (1981) Annu. Rev. Biophys. Bioeng. 10 , 1–67]. We reexamined issue, using buffer system with polyamines that optimized to reproduce in‐vivo ‐like performance in vitro . By slightly modifying this buffer, we were able find conditions stabilize functional ribosomal complexes against dissociating effect up...

Oxygenator thrombosis is a serious complication in extracorporeal membrane oxygenation (ECMO) and may necessitate system exchange. Coagulation fibrinolysis parameters, flow dynamics, gas transfer performance are currently used to evaluate the degree of oxygenator thrombosis, but there no technical approach for direct visualization quantification thrombotic deposits within (MO). We multidetector computed tomography (MDCT) with three-dimensional postprocessing assess incidence quantify...

Various assays of different complexity are used in research on angiogenesis health and disease. The results these increasingly impact the field tissue engineering because preformed microvascular networks may connect conduct to vascular system host, thereby helping us support survival implanted cells constructs. An interesting model that supports formation EC (endothelial cells) tubular structures vitro is based co-culturing them with fibroblasts. Our initial multilayer approach was recently...

Over the past decade, veno-venous extracorporeal membrane oxygenation (vvECMO) has been increasingly utilized in respiratory failure patients. This study presents our institution´s experience focusing on life span of ECMO systems reflecting performance a particular system. A retrospective review database identified 461 adult patients undergoing vvECMO (2010-2017). Patients that required more than one system and survived first exchange >24 hours (n = 139) were included. Life until criteria...

OBJECTIVE: Serum levels of dehydroepiandrosterone (DHEA) and DHEA sulphate (DHEAS) are low in chronic inflammatory diseases, although the reasons unexplained. Furthermore, behaviour serum these hormones during an acute stressful disease state is not well known. SUBJECTS AND METHODS: In this study patients with (13 undergoing cardiothoracic surgery) inflammation (61 bowel diseases (IBD)) vs. 120 controls, we aimed to investigate adrenal hormone shifts looking at relation other hormones....

Abstract Although endothelial cell (EC) seeding improves the patency of vascular prostheses, detachment adherent ECs after restoration circulation remains one major obstacles. Polymer surfaces for endothelialization can be optimized. In this study, polyethylene terephthalate (PET), polypropylene (PP), polytetrafluoroethylene (PTFE), polyurethane (PUR), and silicone were coated with a titaniumcarboxonitride (Ti(C,N,O)) layer by plasma‐assisted chemical vapor deposition process to verify...

Despite heparin coating and systemic anticoagulation, thrombotic clot formation is a serious complication in extracorporeal membrane oxygenation (ECMO). We describe our first results of visualization deposits ECMO devices using advanced multidetector computed tomography (MDCT). A bioline-coated polymethylpentene oxygenator (MO) after 8 days treatment (device 1) factory-sealed MO serving as an internal quality control 2) were analyzed with three-dimensional (3D) volume rendering technique...

The effects of cisplatin on the lung parenchyma during hyperthermic intrathoracic chemotherapy perfusion have not been analysed in detail. objective this study was to evaluate both concentration and depth penetration human tissue after exposure under ex vivo conditions.This experimental approved by local ethics committee. Twelve patients underwent pulmonary wedge resections elective thoracic lobectomies were performed (resected lobe), (approximately 1-2 cm(3)) incubated (in vitro) with (0.05...

Thrombosis is the most common technical complication with extracorporeal membrane oxygenation (ECMO). Accumulations of leukocytes on gas exchange membranes within a oxygenator (MO) may initiate thrombosis and influence outcome. MOs (n = 41) were removed routinely from adult patients ECMO, preserved, analyzed for their cellular deposits using nuclear (4',6-diamidino-2-phenylindole) cell type-specific markers (CD45; von Willebrand factor, vWF). The extent colonization was correlated patient...

Abstract Clot formation within membrane oxygenators (MOs) remains a critical problem during extracorporeal oxygenation (ECMO). The composition of the clots—in particular, presence von Willebrand factor (vWF)—may be an indicator for prevalent nonphysiological flow conditions, foreign body reactions, or coagulation abnormalities in critically ill patients. Mats interwoven gas exchange fibers from randomly collected MOs (PLS, Maquet, Rastatt, Germany) 21 patients were stained with antibodies...

Prevention of the coagulation cascade and platelet activation is foremost demand for biomaterials in contact with blood. In this review we describe underlying mechanisms these processes offer current state antithrombotic strategies. We give an overview methods to prevent protein adhesion, as well techniques immobilize biochemically active molecules on biomaterial surfaces. Finally, recent strategies biofunctionalization by endothelial cell seeding their possible clinical applications are discussed.