A5037484817- Chronic Myeloid Leukemia Treatments
- Epigenetics and DNA Methylation
- Chronic Lymphocytic Leukemia Research
- Prostate Cancer Treatment and Research
- Acute Lymphoblastic Leukemia research
- Cancer, Hypoxia, and Metabolism
- Cancer Research and Treatments
- Ubiquitin and proteasome pathways
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- DNA Repair Mechanisms
- Nanoparticle-Based Drug Delivery
- Acute Myeloid Leukemia Research
- Chemical Reactions and Isotopes
- Molecular Biology Techniques and Applications
- Cancer Cells and Metastasis
- Protein Kinase Regulation and GTPase Signaling
- Cellular Mechanics and Interactions
- Lymphoma Diagnosis and Treatment
- HER2/EGFR in Cancer Research
- Alkaline Phosphatase Research Studies
- Renal and related cancers
- Blood groups and transfusion
- Tissue Engineering and Regenerative Medicine
- Immunodeficiency and Autoimmune Disorders
- Microtubule and mitosis dynamics
Institute of Molecular Biology and Genetics
2015-2025
National Academy of Sciences of Ukraine
2013-2024
Instituto de Biomedicina y Genética Molecular de Valladolid
2020-2022
OncoRay
2016
Technische Universität Dresden
2016
Translational Research in Oncology
2016
University Hospital Carl Gustav Carus
2016
University of Alberta
2016
College of the Holy Cross
2016
Cancer Institute (WIA)
2016
Radiotherapy is a curative treatment option in prostate cancer. Nevertheless, patients with high-risk cancer are prone to relapse. Identification of the predictive biomarkers and molecular mechanisms radioresistance bears promise improve therapies. In this study, we show that aldehyde dehydrogenase (ALDH) activity indicative radioresistant progenitor cells an enhanced DNA repair capacity activation epithelial-mesenchymal transition (EMT). Gene expression profiling cells, their derivatives,...
Tumor progenitor cells represent a population of drug-resistant that can survive conventional chemotherapy and lead to tumor relapse. However, little is known the role progenitors in prostate cancer metastasis. The studies reported herein show CXCR4/CXCL12 axis, key regulator dissemination, plays maintenance stem-like cells. CXCL4/CXCR12 pathway activated CD44(+)/CD133(+) affects differentiation potential, cell adhesion, clonal growth tumorigenicity. Furthermore, xenograft mice showed...
Radiotherapy is a mainstay of curative prostate cancer treatment, but risks recurrence after treatment remain significant in locally advanced disease. Given that tumor relapse can be attributed to population stem cells (CSC) survives radiotherapy, analysis this cell might illuminate tactics personalize treatment. However, direction remains challenging given the plastic nature cancers following We show here irradiating stimulates durable upregulation markers epigenetically reprogram these...
Drug targeting to specific organs and tissues is one of the crucial endeavors modern pharmacotherapy. Controlled at site action reduced time exposure nontargeted increase efficacy treatment reduce toxicity side effects, improving compliance convenience. Nanocarriers based on branched copolymers dextran-graft-polyacrylamide were synthesized characterized tested phagocytic cells. It was shown that these nanoparticles are actively captured by cells they not cytotoxic. The polymer loaded with...
Ubiquitin-specific protease 1 (USP1) is an important deubiquitinating enzyme (DUB) involved in the maintenance of genome integrity, cell cycle, and homeostasis. USP1 overexpression a characteristic feature various cancers, correlating with poor prognosis. The review summarizes recent knowledge understanding role deubiquitinase stabilization oncoproteins tumor suppressors, as critical event cancer development progression. putative mechanisms involvement some prevalent human cancers are...
Summary. Aim: To analyze interaction of ubiquitin specific peptidase 1 (USP1) with Bcr-Abl and to assess the relation between USP1 functional activity expression in K562 chronic myeloid leukemia cells. Materials Methods: The cells was analyzed by co-immunoprecipitation, Western blot analysis, confocal microscopy. Results: A direct oncoprotein protein established co-immunoprecipitation. Immunofluorescence analysis microscopy revealed that Bcr-Abl/USP1 complex is formed cell nucleus....
Chromosomal translocation t(9;22)(q34;q11) leads to generation of different types the BCR-ABL fusion protein and cause leukemia.The generated proteins differ by presence or absence certain domains BCR -C2, PH, DH.Mass-spectrometric analysis identified 23 possible interaction partners PH domain BCR.Among them is cortactin (CTTN), which a multidomain responsible for actin branching during endocytosis.The activation occurs after phosphorylation SRC kinase.However, it unknown whether ABL kinase...
The hallmark of chronic myeloid leukemia (CML) and a subset acute lymphoblastic (ALL) is the presence Philadelphia chromosome as result t(9;22) translocation. This gene rearrangement results in production novel oncoprotein, BCR/ABL, constitutively active tyrosine kinase. There compelling evidence that malignant transformation by BCR/ABL critically dependent on its Abl kinase activity. Also bcr part hybrid takes realization phenotype. We supposed additional mutations accumulate this region...
Summary. Placental-like alkaline phosphatase (PLAP) is expressed by many tumors and can be detected in sera of patients with various cancers. Its aberrant expression has been considered to potentially useful as tumor marker. However, the biological background role this (AP) cancer still unclear. The forms AP cells chronic myeloid leukemia (CML) not yet studied. Aim: To analyze patterns originated from CML blast crisis modify their vitamin E. Materials Methods: RNA extracted leukemic was...
The aim of the work was to create a protocol for detecting V617F mutation gene jak2 in samples patients with chronic myeloproliferative neoplasm which is necessary unify procedures analysis blood according WHO criteria this group diseases.Methods.Mutation revealed using reverse transcriptase PCR and direct sequencing products.Results.Six polycythemia vera were analyzed detected all six cases.This not any RNA healthy donors.A case simultaneous detection mutations fused bcr/abl CML patient...
Background: Chronic myeloid leukemia (CML) is a clonal hematopoietic stem cell disorder associated with the activity of BCR-ABL fusion oncogene. Tyrosine kinase inhibitors are current treatment CML, but secondary mutations finally contribute to therapy resistance and blast crisis disease. The search for novel compounds effective control CML now in spotlight. progression correlated down-modulation C/EBP alpha. Therefore, alpha may be considered as putative target differentiation therapies...
Disruption of the genetic component cells are mandatory element malignant transformation.For majority blood neoplasias disorders have been discovered, and they can be used for diagnosis appropriate therapy.The data obtained by authors about role domains Bcr-Abl protein (the main etiological factor in pathogenesis leukemia with Ph-chromosome) presented this review as well approved diagnostic methods myeloproliferative acute leukemias.
Aim. Malignant neoplasms pose a significant challenge in medicine, requiring deep understanding of the molecular mechanisms underlying their pathogenesis. This study aimed to investigate expression profile and subcellular localization ubiquitin-specific protease 1 (USP1) various types cancer. Methods. Using TCGA database, an analysis USP1 was conducted across different cancer types. Immunofluorescence with confocal microscopy utilized determine chronic myeloid leukemia (CML) prostate cells....