A5003778325- Receptor Mechanisms and Signaling
- Photoreceptor and optogenetics research
- Neuroscience and Neuropharmacology Research
- Alzheimer's disease research and treatments
- Genetic Neurodegenerative Diseases
- Lipid Membrane Structure and Behavior
- Antibiotic Resistance in Bacteria
- Enzyme Structure and Function
- Protein Structure and Dynamics
- Cancer therapeutics and mechanisms
- Computational Drug Discovery Methods
- Hereditary Neurological Disorders
- Ionic liquids properties and applications
- Protease and Inhibitor Mechanisms
- Inflammatory mediators and NSAID effects
- Mycorrhizal Fungi and Plant Interactions
- Microtubule and mitosis dynamics
- Mitochondrial Function and Pathology
- Medicinal plant effects and applications
- Structural and Chemical Analysis of Organic and Inorganic Compounds
- Lung Cancer Research Studies
- Bacteriophages and microbial interactions
- Fungal Biology and Applications
- Neutropenia and Cancer Infections
- Force Microscopy Techniques and Applications
University of Warsaw
2004-2023
Polish Academy of Sciences
2014-2015
Mossakowski Medical Research Institute, Polish Academy of Sciences
2014
International Institute of Molecular and Cell Biology
2004-2012
MMP-9 plays a number of important physiological functions but is also responsible for many pathological processes, including cancer invasion, metastasis, and angiogenesis. It is, therefore, crucial to understand its enzymatic activity, activation inhibition mechanisms. This enzyme may be partially involved in the "cytokine storm" that characteristic COVID-19 disease (SARS-CoV-2), as well molecular mechanisms lung fibrosis. Due variety processing pathways involving biological systems...
To investigate the mechanistic basis of anti-tumor effect compound ITB-301.Chemical modifications genistein have been introduced to improve its solubility and efficacy. The effects were tested in ovarian cancer cells using proliferation assays, cell cycle analysis, immunofluorescence, microscopy.In this work, we show that a unique glycoside genistein, ITB-301, inhibits SKOv3 cells. We found 50% growth inhibitory concentration ITB-301 was 0.5 μM. Similar results obtained breast cancer, acute...
Charcot-Marie-Tooth disease type 2A (CMT2A) is an autosomal dominant axonal peripheral neuropathy caused by mutations in the mitofusin 2 gene (MFN2). Mitofusin a GTPase protein present outer mitochondrial membrane and responsible for regulation of network architecture via fusion mitochondria. As that process known to be strongly dependent on activity 2, it postulated MFN2 mutation within domain may lead impaired activity, turn dysfunction. The work described here has therefore sought verify...
Vasoactive intestinal peptide receptor 1 (VPAC1) is a member of secretin-like subfamily G protein-coupled receptors. Its endogenous neuropeptide (VIP), secreted by neurons and immune cells, modulates various physiological functions such as exocrine endocrine secretions, response, smooth muscles relaxation, vasodilation, fetal development. As drug target, VPAC1 has been selected for therapy inflammatory diseases but discovery still hampered lack its crystal structure. In this study we...
Abstract Charcot-Marie-Tooth disease type 2A (CMT2A) is an autosomal dominant neuropathy caused by mutations in the mitofusin 2 gene ( MFN2 ). More than 100 have been reported so far, with majority located within GTPase domain encoding region. These domain-specific present wide range of symptoms differences associated distinct amino acid substitutions same position. Due to lack conclusive phenotype-genotype correlation predictive value genetic results remains still limited. We explored...
Human DNA topoisomerase I (topo I) catalyzes relaxation and phosphorylates SRSF1. Whereas the structure of topo complexed with has been resolved, in complex SRSF1 structural determinants activities this are not known. The main obstacle to resolving is a contribution unfolded domains formation complex. To overcome difficulty, we employed three-step strategy: identifying interaction regions, modeling complex, validating model biochemical methods. binding sites both localized structured regions...
Recently, molecular covalent docking has been extensively developed to design new classes of inhibitors that form chemical bonds with their biological targets. This strategy for the such inhibitors, in particular boron-based holds great promise vast family β-lactamases produced, inter alia , by Gram-negative antibiotic-resistant bacteria. However, description processes requires a quantum-mechanical approach, and so far, only few studies this type have presented. study accurately describes...
Gamma-secretase is an integral membrane protease, which a complex of four proteins. Improper functioning gamma-secretase was found to be critical in the pathogenesis Alzheimer's disease. Despite numerous efforts, structure protease as well its proteolytic mechanism remains poorly understood. In this work we constructed model interactions between two proteins forming gamma-secretase: APH-1 and presenilin. This interface based on highly conserved GxxxGxxxG motif protein. It can form tight...
Over 30 compounds, including para-, meta-, and ortho-phenylenediboronic acids, ortho-substituted phenylboronic benzenetriboronic di- triboronated thiophenes, pyridine derivatives were investigated as potential β-lactamase inhibitors. The highest activity against KPC-type carbapenemases was found for acid 3a, which at the concentration of 8/4 mg/L reduced carbapenems’ MICs up to 16/8-fold, respectively. Checkerboard assays revealed strong synergy between carbapenems 3a with fractional...
Predicting FRET pathways in proteins using computer simulation techniques is very important for reliable interpretation of experimental data. A novel and relatively simple methodology has been developed applied to purine nucleoside phosphorylase (PNP) complexed with a fluorescent ligand - formycin (FA). occurs between an excited Tyr residue (D*) FA (A). This study aims interpret data that, among others, suggests the absence PNPF159A mutant complex FA, based on theoretical methodology. MD...
Scaffold-based analogs of cinnamic acid benzyl amide (CABA) exhibit pleiotropic effects in cancer cells, and their exact molecular mechanism action is under investigation. The present study part our systemic analysis interactions CABA with targets. These compounds were shown to inhibit Janus kinase 2 (JAK2)/signal transducer activator transcription 3 (STAT3) JAK2/signal 5 (STAT5) signaling thus are attractive scaffolds for anticancer drug design. To identify the potential mechanisms this...
The TM4–TM5 model of higher-order structure rhodopsin (a structural and functional template for G protein-coupled receptors, GPCRs) in the membrane was used to investigate a complex between oligomeric (Rh) its protein (transducin, Gt). Comparison with currently available models organization GPCRs made, is discussed experimental data which revealed flexible intradimeric Rh interface. We also characterized membranous anchor domain Gt Rh–Gt, critically important binding transducin Rh.