A5030626364- Neuroscience and Neuropharmacology Research
- Mitochondrial Function and Pathology
- Metabolism and Genetic Disorders
- Adipose Tissue and Metabolism
- Neuroinflammation and Neurodegeneration Mechanisms
- Biochemical effects in animals
- Ion channel regulation and function
- ATP Synthase and ATPases Research
- Muscle Physiology and Disorders
- Calpain Protease Function and Regulation
- Growth Hormone and Insulin-like Growth Factors
- Neurological diseases and metabolism
- Epilepsy research and treatment
- Amino Acid Enzymes and Metabolism
- Nuclear Receptors and Signaling
- Autophagy in Disease and Therapy
- Nitric Oxide and Endothelin Effects
- Neuropeptides and Animal Physiology
- Neurological Disease Mechanisms and Treatments
- Hereditary Neurological Disorders
- Lipid Membrane Structure and Behavior
- Neurological Disorders and Treatments
- Cell death mechanisms and regulation
- Melanoma and MAPK Pathways
- Genetic Neurodegenerative Diseases
Mossakowski Medical Research Institute, Polish Academy of Sciences
2014-2025
Polish Academy of Sciences
2013-2025
International Institute of Molecular and Cell Biology
2021
Clinical Trial Investigators
2009
Medical University of Warsaw
2001
Postgraduate School of Molecular Medicine
1999
Institute of Psychiatry and Neurology
1999
Laboratory of Molecular Genetics
1998
National Institute of Mental Health
1992
Instytut Biologii Doświadczalnej im. Marcelego Nenckiego
1989-1990
Neural stem cells are clonogenic, self-renewing with the potential to differentiate into brain-specific cell lines. Our study demonstrates that a neural-stem-cell-like subpopulation can be selected and expanded in vitro by use of human umbilical cord blood cells, which relatively easily available starting material. Through combination antigen-driven magnetic sorting subfractionation according surface adhesive properties, we have isolated clonogenic fraction devoid hematopoietic or...
One of the key factors that may influence therapeutic potential mesenchymal stem/stromal cells (MSCs) is their metabolism. The switch between mitochondrial respiration and glycolysis can be affected by many factors, including oxygen concentration spatial form culture. This study compared metabolic features adipose-derived (ASCs) dedifferentiated fat (DFATs) cultivated as monolayer or spheroid culture under 5% O2 (physiological normoxia) impact on MSCs abilities.We observed cultured spheroids...
The ischemic stroke is the third leading cause of death in developed countries. C-terminal peptide mechano-growth factor (MGF), an alternatively spliced variant insulin-like growth 1 (IGF-1), was found to function independently from rest molecule and showed a neuroprotective effect vivo vitro. In vivo, gerbil model transient brain ischemia, treatment with synthetic MGF provided very significant protection vulnerable neurons. same model, ischemia evoked increased expression endogenous...
Twelve depressant drugs were studied for their effects upon presynaptic inhibition in the spinal cord unanesthetized cats. Eight anesthetic agents (pentobarbital, phenobarbital, ethanol, chlorai hydrate, chloroform, diethyl ether, nitrous oxide and magnesium sulfate) all markedly enhanced doses that produced sedation or light anesthesia. This effect was also seen with anticonvulsant drug trimethadione anticonvulsant, nontoxic doses. Three other depressants had different inhibition....
Charcot-Marie-Tooth disease type 2A (CMT2A) is an untreatable childhood peripheral neuropathy caused by mutations of the mitochondrial fusion protein, mitofusin (MFN) 2. Here, pharmacological activation endogenous normal mitofusins overcame dominant inhibitory effects CMT2A mutants in reprogrammed human patient motor neurons, reversing hallmark stasis and fragmentation independent causal MFN2 mutation. In mice expressing T105M, intermittent with a small molecule, MiM111, normalized...
Mitofusin 2 (Mfn2), mitochondrial outer membrane protein which is involved in rearrangement of these organelles, was first described pathology hypertension and diabetes, more recently much attention paid to its functions Charcot-Marie-Tooth type 2A neuropathy (CMT2A). Here, cellular energy metabolism investigated mouse embryonic fibroblasts (MEF) differing the presence Mfn2 gene; control (MEFwt) with gene depleted MEFMfn2-/-. These two cell lines were compared terms various parameters...
Background: Exposure to ozone level and ultraviolet (UV) radiation is one of the major concerns in context public health. Numerous studies confirmed that abundant free radicals initiate undesired processes, e.g. carcinogenesis, cells degeneration, etc. Therefore, design redox-active molecules with novel structures, containing radical quenchers understanding their chemistry biology, might be prospective solutions. Methods: We designed a group peptide dendrimers carrying multiple copies...
Abstract Duchenne muscular dystrophy (DMD) is characterized by progressive muscle degeneration and neuropsychiatric abnormalities. Loss of full-length dystrophins both necessary sufficient to initiate DMD. These isoforms are expressed in the hippocampus, cerebral cortex (Dp427c), cerebellar Purkinje cells (Dp427p). However, our understanding consequences their absence, which crucial for developing targeted interventions, remains inadequate. We combined RNA sequencing with genome-scale...
Energy metabolism homeostasis emerges as a dominant element influencing mesenchymal stem/stromal cells' trajectory of development. The predominant glycolysis activity is primary driver cell proliferation and maintenance the high-energetic state. Here, we examined functions two crucial auxiliary pathways: phosphate-pentose pathway (PPP) fructose-2,6-biphosphate (FBP) to evaluate their impact on therapeutic potential Adipose-Derived Stem/Stromal cells (ASCs) during prolonged culture in various...
Biphalin is a dimeric opioid peptide that exhibits affinity for three types of receptors (MOP, DOP and KOP). undergoing intensive preclinical study. It was recognized activation δ-opioid receptor elicits neuroprotection against brain hypoxia ischemia. We compare the effect biphalin morphine inhibition by naltrexone on survival neurons in rat organotypic hippocampal cultures challenged with NMDA. Findings: (1) 0.025–0.1 μM reduces NMDA-induced neuronal damage; (2) abolished naltrexone; (3)...
Mitochondrial fusion is essential to mitochondrial fitness and cellular health. Neurons of patients with genetic neurodegenerative diseases often exhibit fragmentation, reflecting an imbalance in fission (mitochondrial dysdynamism). Charcot-Marie-Tooth (CMT) disease type 2A the prototypical disorder impaired caused by mutations protein mitofusin (MFN)2. Yet, cultured CMT2A patient fibroblast mitochondria are reported as morphologically normal. Metabolic stress might evoke pathological...
Abstract In attempts to develop effective therapeutic strategies limit post-ischemic injury, mitochondria emerge as a key element determining neuronal fate. Mitochondrial damage can be alleviated by various mechanisms including mitochondrial network remodelling, elimination and protein biogenesis. However, the regulating relationships between these phenomena are poorly understood. We hypothesized that mitofusin 2 (Mfn2), GTPase involved in fusion, trafficking endoplasmic reticulum (ER)...